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Venom fractionation was performed using three chromatographic steps (molecular exclusion on Sephadex G\u201075, ion\u2010exchange on DEAE\u2010Sephadex A\u201050, and reversed\u2010phase high\u2010performance liquid chromatography on C8 column). CcD\u2010II presented an apparent molecular mass of 39.9 kDa and displayed a dermonecrotic activity with a minimal necrotic dose of 0.2 mg\/kg body weight. CcD\u2010II showed proteolytic ability on casein chains and on \u03b1 and \u03b2 fibrinogen chains that was inhibited by ethylenediamine tetraacetic acid and 1,10\u2010phenanthroline while remained unaffected by phenylmethylsulphonyl fluoride and heparin. CcD\u2010II displayed gelatinase activity and degraded extracellular matrix compounds (type\u2010IV collagen and laminin). These results correlated with histopathological analysis showing a complete disorganization of collagenous skin fibers. These data suggested that CcD\u2010II belongs to P\u2010II class of snake venom metalloproteinase. The characterization of venom compounds involved in tissue damage may contribute in the development of new therapeutic strategies in envenomation.<\/jats:p>","DOI":"10.1002\/jbt.21835","type":"journal-article","created":{"date-parts":[[2016,9,2]],"date-time":"2016-09-02T13:04:01Z","timestamp":1472821441000},"source":"Crossref","is-referenced-by-count":9,"title":["Biochemical and biological characterization of a dermonecrotic metalloproteinase isolated from <i>Cerastes cerastes<\/i> snake venom"],"prefix":"10.1002","volume":"31","author":[{"given":"Amina","family":"Ami","sequence":"first","affiliation":[{"name":"USTHB, Faculty of Biological Sciences Laboratory of Cellular and Molecular Biology  Bab Ezzouar Algiers Algeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Habiba","family":"Oussedik\u2010Oumehdi","sequence":"additional","affiliation":[{"name":"USTHB, Faculty of Biological Sciences Laboratory of Cellular and Molecular Biology  Bab Ezzouar Algiers Algeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fatima","family":"Laraba\u2010Djebari","sequence":"additional","affiliation":[{"name":"USTHB, Faculty of Biological Sciences Laboratory of Cellular and Molecular Biology  Bab Ezzouar Algiers Algeria"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2016,9,2]]},"reference":[{"key":"e_1_2_6_2_1","volume-title":"Central and South America: Epidemiology, clinical features and clinical management","author":"Warrell D. 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