{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T02:51:51Z","timestamp":1792464711668,"version":"4.5.0"},"reference-count":51,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2016,12,29]],"date-time":"2016-12-29T00:00:00Z","timestamp":1482969600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Biochem &amp; Molecular Tox"],"published-print":{"date-parts":[[2017,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Serum paraoxonase (PON1) is a key enzyme related to high\u2010density lipoprotein (HDL)\u2010cholesterol particle. It can prevent the oxidation of low\u2010density lipoprotein (LDL) and HDL. The present article focuses on the <jats:italic>in vitro<\/jats:italic> inhibition role of some antiepileptic drugs (AEDs) such as valproic acid, gabapentin, primidone, phenytoin, and levetiracetam on human paraoxonase (hPON1). Therefore, PON1 was purified from human serum with a specific activity of 3976.36\u00a0EU\/mg and 13.96% yield by using simple chromatographic methods. The AEDs were tested at various concentrations, which showed reduced <jats:italic>in vitro<\/jats:italic> hPON1 activity. IC<jats:sub>50<\/jats:sub> values for gabapentin, valproic acid, primidone, phenytoin, and levetiracetam were found to be 0.35, 0.67, 0.87, 6.3, and 53.3\u00a0mM, respectively. <jats:italic>K<\/jats:italic><jats:sub>i<\/jats:sub> constants were 0.261\u00a0\u00b1\u00a00.027, 0.338\u00a0\u00b1\u00a00.313, 0.410\u00a0\u00b1\u00a00.184, 10.3\u00a0\u00b1\u00a00.001, and 43.01\u00a0\u00b1\u00a00.003\u00a0mM, respectively. Gabapentin exhibited effective inhibitory activity as compared with the other drugs. The inhibition mechanisms of all compounds were noncompetitive.<\/jats:p>","DOI":"10.1002\/jbt.21889","type":"journal-article","created":{"date-parts":[[2016,12,29]],"date-time":"2016-12-29T12:32:43Z","timestamp":1483014763000},"source":"Crossref","is-referenced-by-count":70,"title":["Antiepileptic drugs: Impacts on human serum paraoxonase\u20101"],"prefix":"10.1002","volume":"31","author":[{"given":"\u015e\u00fckr\u00fc","family":"Beydemir","sequence":"first","affiliation":[{"name":"Department of Biochemistry, Faculty of Pharmacy Anadolu University  Eski\u015fehir 26470 Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yeliz","family":"Demir","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Faculty of Sciences Atat\u00fcrk University  Erzurum 25240 Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2016,12,29]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1212\/WNL.0b013e31822cfc90"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/epi.12605"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/epi.12579"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1212\/01.wnl.0000345667.45642.61"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0404.2005.00507.x"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-444-52891-9.00073-7"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1177\/088307380401900104"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.mp.4001087"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1586\/ern.10.3"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.2165\/11316250-000000000-00000"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/path.2643"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpurol.2011.10.022"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1440-1819.2004.01244_1.x"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11936-013-0236-7"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.2165\/11596280-000000000-00000"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/cbdd.12746"},{"key":"e_1_2_5_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/cbdd.12561"},{"key":"e_1_2_5_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbi.2010.01.039"},{"key":"e_1_2_5_20_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11883-009-0029-3"},{"issue":"11","key":"e_1_2_5_21_1","first-page":"1020","volume":"17","author":"Razavi A. E.","year":"2012","journal-title":"J Res Med Sci"},{"key":"e_1_2_5_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(91)80962-3"},{"key":"e_1_2_5_23_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M107440200"},{"issue":"12","key":"e_1_2_5_24_1","first-page":"1986","volume":"37","author":"James R. W.","year":"2004","journal-title":"Biol Med"},{"key":"e_1_2_5_25_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.ATV.21.4.473"},{"key":"e_1_2_5_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.eplepsyres.2007.03.010"},{"key":"e_1_2_5_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1734-1140(09)70082-9"},{"key":"e_1_2_5_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2006.03.029"},{"key":"e_1_2_5_29_1","doi-asserted-by":"publisher","DOI":"10.3906\/kim-1501-51"},{"key":"e_1_2_5_30_1","doi-asserted-by":"publisher","DOI":"10.1080\/14756360802608351"},{"key":"e_1_2_5_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pestbp.2011.09.007"},{"key":"e_1_2_5_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejphar.2010.07.030"},{"key":"e_1_2_5_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(76)90527-3"},{"key":"e_1_2_5_34_1","doi-asserted-by":"publisher","DOI":"10.1038\/227680a0"},{"key":"e_1_2_5_35_1","doi-asserted-by":"publisher","DOI":"10.1021\/ja01318a036"},{"key":"e_1_2_5_36_1","doi-asserted-by":"publisher","DOI":"10.1039\/C3AY41527A"},{"key":"e_1_2_5_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/0021-9150(93)90183-U"},{"key":"e_1_2_5_38_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.275.6.3957"},{"key":"e_1_2_5_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cccn.2004.09.019"},{"key":"e_1_2_5_40_1","doi-asserted-by":"publisher","DOI":"10.1093\/clinchem\/32.4.671"},{"key":"e_1_2_5_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.arcmed.2012.08.012"},{"issue":"2","key":"e_1_2_5_42_1","first-page":"229","volume":"22","author":"Krzystek\u2010Korpacka M.","year":"2013","journal-title":"Adv Clin Exp Med"},{"key":"e_1_2_5_43_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00204-011-0774-2"},{"key":"e_1_2_5_44_1","first-page":"408","volume":"13","author":"G\u00fcl\u00e7in \u0130.","year":"2013","journal-title":"Mini Rev Med Chem"},{"key":"e_1_2_5_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neulet.2013.10.040"},{"key":"e_1_2_5_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.eplepsyres.2007.03.010"},{"key":"e_1_2_5_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1734-1140(09)70082-9"},{"key":"e_1_2_5_48_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1528-1167.2009.02024.x"},{"key":"e_1_2_5_49_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.eplepsyres.2013.08.016"},{"key":"e_1_2_5_50_1","doi-asserted-by":"publisher","DOI":"10.1097\/WNF.0b013e3181d1e133"},{"key":"e_1_2_5_51_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12013-015-0683-8"},{"key":"e_1_2_5_52_1","doi-asserted-by":"publisher","DOI":"10.3109\/13813455.2015.1026912"}],"container-title":["Journal of Biochemical and Molecular Toxicology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjbt.21889","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjbt.21889","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jbt.21889","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,4]],"date-time":"2023-10-04T02:45:35Z","timestamp":1696387535000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jbt.21889"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,12,29]]},"references-count":51,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2017,6]]}},"alternative-id":["10.1002\/jbt.21889"],"URL":"https:\/\/doi.org\/10.1002\/jbt.21889","archive":["Portico"],"relation":{},"ISSN":["1095-6670","1099-0461"],"issn-type":[{"value":"1095-6670","type":"print"},{"value":"1099-0461","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,12,29]]},"article-number":"e21889"}}