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In this study, a new hemorrhagin, Cc HSM\u2010III (66 kDa), was purified from <jats:italic>Cerastes cerastes<\/jats:italic> venom by gel filtration, ion exchange, and reversed\u2010phase high\u2010performance liquid chromatographies. The analysis of Cc HSM\u2010III by liquid chromatography with a tandem mass spectrometry revealed 32 peptides sharing a homology with P\u2010III metalloproteinases from <jats:italic>Echis ocellatus<\/jats:italic> snake venom. Cc HSM\u2010III displays hemorrhagic activity with a minimal hemorrhagic dose of 5 \u03bcg, which is abolished by ethylene diamine tetracetic acid but not by phenylmethylsulfonyl fluoride. The mechanism underlying Cc HSM\u2010III hemorrhagic activity is probably due to its extensive proteolytic activity against type IV collagen. Cc HSM\u2010III induces local tissue damage and an inflammatory response by upregulating both matrix metalloproteinase 2 and 9 in skin of mice. Thus, Cc HSM\u2010III may play a key role in the pathogenesis of <jats:italic>C. cerastes<\/jats:italic> envenomation.<\/jats:p>","DOI":"10.1002\/jbt.21899","type":"journal-article","created":{"date-parts":[[2017,2,28]],"date-time":"2017-02-28T13:46:12Z","timestamp":1488289572000},"source":"Crossref","is-referenced-by-count":10,"title":["Hemorrhagic metalloproteinase, Cc HSM\u2010III, isolated from <i>Cerastes cerastes<\/i> venom: Purification and biochemical characterization"],"prefix":"10.1002","volume":"31","author":[{"given":"Wafa","family":"Tachoua","sequence":"first","affiliation":[{"name":"USTHB, Faculty of Biological Sciences Laboratory of Cellular and Molecular Biology, BP 32  El\u2010Alia Bab Ezzouar 16111 Algiers Algeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hinda","family":"Boukhalfa\u2010Abib","sequence":"additional","affiliation":[{"name":"USTHB, Faculty of Biological Sciences Laboratory of Cellular and Molecular Biology, BP 32  El\u2010Alia Bab Ezzouar 16111 Algiers Algeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fatima","family":"Laraba\u2010Djebari","sequence":"additional","affiliation":[{"name":"USTHB, Faculty of Biological Sciences Laboratory of Cellular and Molecular Biology, BP 32  El\u2010Alia Bab Ezzouar 16111 Algiers Algeria"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,2,28]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.200402024"},{"key":"e_1_2_7_3_1","first-page":"1269","volume":"34","author":"Jia L. 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