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The molecular mass of the protein was found to be 13,659.65\u00a0Da, and peptide mass fingerprinting revealed that it belongs to group II PLA<jats:sub>2<\/jats:sub> family. The peptide sequence showed similarity to uncharacterized basic PLA<jats:sub>2<\/jats:sub> enzyme having an accession no. of P86368 reported from Sri Lankan <jats:italic>D. russelii<\/jats:italic>. Rv(i) PLA<jats:sub>2<\/jats:sub> exhibited strong phospholipase A<jats:sub>2<\/jats:sub> and anticoagulant activity. It also induced expression of COX\u20102 and TNF\u2010\u03b1 mRNA in a dose\u2010dependent manner in phorbol 12\u2010myristate 13\u2010acetate differentiated THP\u20101 cells, which play a crucial role during inflammation. Chemical modification of His residue in Rv(i) PLA<jats:sub>2<\/jats:sub> with <jats:italic>p<\/jats:italic>\u2010bromophenacyl bromide abolished the enzymatic, anticoagulant, and inflammatory activities. The result indicates that the catalytic site of Rv(i) PLA<jats:sub>2<\/jats:sub> might play a vital role in inducing inflammation at the bite site during <jats:italic>D. russelii<\/jats:italic> envenomation.<\/jats:p>","DOI":"10.1002\/jbt.21945","type":"journal-article","created":{"date-parts":[[2017,6,13]],"date-time":"2017-06-13T10:52:45Z","timestamp":1497351165000},"source":"Crossref","is-referenced-by-count":20,"title":["Purification and partial characterization of an anticoagulant PLA<sub>2<\/sub> from the venom of Indian <i>Daboia russelii<\/i> that induces inflammation through upregulation of proinflammatory mediators"],"prefix":"10.1002","volume":"31","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6731-9716","authenticated-orcid":false,"given":"Archana","family":"Deka","sequence":"first","affiliation":[{"name":"Molecular Toxinology Laboratory Department of Molecular Biology and Biotechnology Tezpur University  Tezpur India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maitreyee","family":"Sharma","sequence":"additional","affiliation":[{"name":"Molecular Toxinology Laboratory Department of Molecular Biology and Biotechnology Tezpur University  Tezpur India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manoj","family":"Sharma","sequence":"additional","affiliation":[{"name":"Cellular, Molecular and Environmental Biotechnology Laboratory Department of Molecular Biology and Biotechnology Tezpur University  Tezpur India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rupak","family":"Mukhopadhyay","sequence":"additional","affiliation":[{"name":"Cellular, Molecular and Environmental Biotechnology Laboratory Department of Molecular Biology and Biotechnology Tezpur University  Tezpur India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robin","family":"Doley","sequence":"additional","affiliation":[{"name":"Molecular Toxinology Laboratory Department of Molecular Biology and Biotechnology Tezpur University  Tezpur India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,6,13]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0000603"},{"key":"e_1_2_6_3_1","first-page":"1","volume-title":"Snakes of India: The field guide","author":"Whitaker R.","year":"2004"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.toxicon.2015.06.027"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jprot.2015.08.017"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1580\/06-WEME-CO-023R1.1"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.3109\/15569543.2013.855789"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/0041-0101(88)90216-4"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0304-4165(99)00053-7"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbapap.2007.04.012"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jprot.2016.06.001"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1134\/S0006297909030055"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.toxicon.2011.01.006"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1186\/1472-6807-7-82"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0153770"},{"key":"e_1_2_6_16_1","first-page":"173","volume-title":"Handbook of venoms and toxins reptiles","author":"Doley R.","year":"2009"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.toxicon.2015.02.014"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.toxicon.2003.11.006"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbapap.2014.09.015"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10565-008-9106-6"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.2174\/092986609788923356"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.2174\/092986609788923310"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/227680a0"},{"key":"e_1_2_6_24_1","first-page":"17","volume":"2","author":"Maduwage K.","year":"2011","journal-title":"J. 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