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CA purification was composed of preparation of the hemolysate and conducting the Sepharose\u20104B\u2010tyrosine\u2010sulfanilamide affinity gel chromatography in having specific activity of 11626\u00a0EU\u00a0mg<jats:sup>\u22121<\/jats:sup>, yield of 14.40%, and 242.76\u2010fold purification. Sodium dodecyl sulfate\u2010polyacrylamide gel electrophoresis was performed to assess the enzyme purity and a single band was observed. Some antibiotics were exhibited<jats:italic>in vitro<\/jats:italic>inhibition on the CA activity. IC<jats:sub>50<\/jats:sub>values of these inhibitors were calculated by plotting activity percentage. IC<jats:sub>50<\/jats:sub>values of certain drugs (dexamethasone; caffeine; metamizole sodium; tetramisol; ceftiofur HCl; ivermectin; tavilin 50; penokain G; neosym; and sulfamezathine) were found as 0.38, 8.24, 285.53, 114.77, 5.33, 2.76, 27.58, 213.50, 208.28, and 36.60\u00a0\u03bcM, respectively.<jats:italic>K<\/jats:italic><jats:sub>i<\/jats:sub>values of different drugs on Kangal Akkaraman sheep blood CA activity were found in the range of 0.21 \u00b1 0.038\u2013266.64 \u00b1 37.11\u00a0\u03bcM.<\/jats:p>","DOI":"10.1002\/jbt.22000","type":"journal-article","created":{"date-parts":[[2017,9,28]],"date-time":"2017-09-28T16:19:05Z","timestamp":1506615545000},"source":"Crossref","is-referenced-by-count":15,"title":["Inhibitory effects of some drugs on carbonic anhydrase enzyme purified from Kangal Akkaraman sheep in Sivas, Turkey"],"prefix":"10.1002","volume":"32","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8710-2912","authenticated-orcid":false,"given":"\u00dcmit M.","family":"Ko\u00e7yi\u011fit","sequence":"first","affiliation":[{"name":"Vocational School of Health Services Cumhuriyet University Sivas 58140 Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sevgi","family":"Durna Da\u015ftan","sequence":"additional","affiliation":[{"name":"Department of Animal Nutrition and Zootechnics, Division of Biometry and Genetics, Faculty of Veterinary Medicine Cumhuriyet University Sivas 58140 Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3171-0633","authenticated-orcid":false,"given":"Parham","family":"Taslimi","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Faculty of Sciences Atat\u00fcrk University Erzurum 25240 Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Taner","family":"Da\u015ftan","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Faculty of Arts and Sciences Bingol University Bingol 12000 Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5993-1668","authenticated-orcid":false,"given":"\u0130lhami","family":"G\u00fcl\u00e7in","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Faculty of Sciences Atat\u00fcrk University Erzurum 25240 Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,9,28]]},"reference":[{"key":"e_1_2_8_2_1","first-page":"969","volume-title":"Sheep breeding","author":"Akcapnar H.","year":"2000"},{"key":"e_1_2_8_3_1","volume-title":"Turkiye pet animal genetic resources introduction catalog","author":"General Directorate of Agricultural Research and Policies (TAGEM)","year":"2009"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.smallrumres.2005.08.013"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1080\/14756360701474780"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bmc.2009.01.067"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bmc.2009.07.019"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1747-0285.2010.00965.x"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bmc.2010.01.076"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bmcl.2010.07.038"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1747-0285.2011.01104.x"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bmcl.2011.05.071"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.3109\/14756366.2012.670807"},{"key":"e_1_2_8_14_1","first-page":"408","volume":"13","author":"G\u00fcl\u00e7in \u0130.","year":"2013","journal-title":"Mini Rev. 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