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The title compounds were prepared starting from readily available pyridine\u20102,5\u2010dicarboxylic acid. The reaction carboxylic acid with absolute ethanol afforded the corresponding dimethyl pyridine\u20102,5\u2010dicarboxylate (<jats:bold>1<\/jats:bold>). The reaction of dimethyl\u20102,5\u2010pyridinedicarboxylate (<jats:bold>1<\/jats:bold>) with hydrazine hydrate good yielded pyridine\u20102,5\u2010dicarbohydrazide (<jats:bold>2<\/jats:bold>). Refluxing compound 2 with alkyl\/aryl isothiocyanate derivatives for 3\u20138\u00a0h afforded 1,4\u2010disubstituted thiosemicarbazides (<jats:bold>3a\u2013e<\/jats:bold>). Base\u2010catalyzed intra\u2010molecular dehydrative cyclization of these intermediates furnished the 4,5\u2010disubstituted bis\u2010mercaptotriazoles (<jats:bold>4a\u2013e<\/jats:bold>) in good yield (85%\u201395%). Among the target compounds, 2,2\u2032\u2010(pyridine\u20102,5\u2010diyldicarbonyl)bis[N\u2010(p\u2010methoxyphenyl)hydrazinecarbothioamide] (<jats:bold>3c<\/jats:bold>) showed very high activity with value of 72.93% against 1,1\u2010diphenyl\u20102\u2010picrylhydrazyl free radical at the concentration of 25\u00a0\u03bcg\/mL. The inhibitory effects of the target compounds against acetylcholinesterase (AChE), hCA I, and II were studied. AChE, cytosolic hCA I and II isoforms were potently inhibited by synthesized these derivatives with <jats:italic>K<\/jats:italic><jats:sub>i<\/jats:sub>s in the range of 3.07\u00a0\u00b1\u00a00.76\u201387.26\u00a0\u00b1\u00a029.25\u00a0nM against AChE, in the range of 1.47\u00a0\u00b1\u00a00.37\u201310.06\u00a0\u00b1\u00a02.96\u00a0nM against hCA I, and in the range of 3.55\u00a0\u00b1\u00a00.57\u20137.66\u00a0\u00b1\u00a02.06\u00a0nM against hCA II, respectively.<\/jats:p>","DOI":"10.1002\/jbt.22006","type":"journal-article","created":{"date-parts":[[2017,11,13]],"date-time":"2017-11-13T14:58:39Z","timestamp":1510585119000},"source":"Crossref","is-referenced-by-count":102,"title":["Synthesis of some novel pyridine compounds containing bis\u20101,2,4\u2010triazole\/thiosemicarbazide moiety and investigation of their antioxidant properties, carbonic anhydrase, and acetylcholinesterase enzymes inhibition profiles"],"prefix":"10.1002","volume":"32","author":[{"given":"Nilufer","family":"Bulut","sequence":"first","affiliation":[{"name":"Department of Chemistry, Faculty of Science and Art Bingol University  Bingol 12000 Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8710-2912","authenticated-orcid":false,"given":"Umit M.","family":"Kocyigit","sequence":"additional","affiliation":[{"name":"Vocational School of Health Services Cumhuriyet University  Sivas 58140 Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ibrahim H.","family":"Gecibesler","sequence":"additional","affiliation":[{"name":"Laboratory of Natural Product Research, Faculty of Health Sciences Bingol University  Bingol 12000 Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Taner","family":"Dastan","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Faculty of Science and Art Bingol University  Bingol 12000 Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huseyin","family":"Karci","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Faculty of Science and Art Bingol University  Bingol 12000 Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3171-0633","authenticated-orcid":false,"given":"Parham","family":"Taslimi","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Faculty of Science Ataturk University  Erzurum 25240 Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sevgi","family":"Durna Dastan","sequence":"additional","affiliation":[{"name":"Department of Biometrics and Genetics, Faculty of Veterinary Medicine Cumhuriyet University  Sivas 58140 Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5993-1668","authenticated-orcid":false,"given":"Ilhami","family":"Gulcin","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Faculty of Science Ataturk University  Erzurum 25240 Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ahmet","family":"Cetin","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Faculty of Science and Art Bingol University  Bingol 12000 Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,11,13]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/jhet.5570270424"},{"key":"e_1_2_7_3_1","first-page":"59","volume":"9","author":"Shaker R. 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