{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T14:46:43Z","timestamp":1786978003414,"version":"build-2736575974"},"reference-count":63,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2018,7,9]],"date-time":"2018-07-09T00:00:00Z","timestamp":1531094400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100002966","name":"Cumhuriyet \u00dcniversitesi","doi-asserted-by":"publisher","award":["SHMYO\u2010011"],"award-info":[{"award-number":["SHMYO\u2010011"]}],"id":[{"id":"10.13039\/501100002966","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Biochem &amp; Molecular Tox"],"published-print":{"date-parts":[[2018,8]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    In this work, the carbonic anhydrase (CA) enzyme was purified from Kangal Akkaraman sheep in Sivas, Turkey with specific activity value of 6681.57\u00a0EU\/mg and yield of 14.90% with using affinity column chromatography. For designating the subunit molecular mass and enzyme purity, sodium dodecyl sulfate polyacrylamide gel electrophoresis method was used and single band for this procedure was obtained. The molecular mass of CA enzyme was found as 28.89\u00a0kDa. In this study, the optimum temperature and optimum pH were obtained from 30 and 7.5.\n                    <jats:italic>V<\/jats:italic>\n                    <jats:sub>max<\/jats:sub>\n                    and\n                    <jats:italic>K<\/jats:italic>\n                    <jats:sub>m<\/jats:sub>\n                    values for p\u2010nitrophenylacetate substrate of the CA were determined from Lineweaver\u2013Burk graphs. Additionally, the inhibitory results of diverse heavy metal ions (Hg\n                    <jats:sup>+<\/jats:sup>\n                    , Fe\n                    <jats:sup>2+<\/jats:sup>\n                    , Pb\n                    <jats:sup>2+<\/jats:sup>\n                    , Co\n                    <jats:sup>2+<\/jats:sup>\n                    , Ag\n                    <jats:sup>+<\/jats:sup>\n                    , and Cu\n                    <jats:sup>2+<\/jats:sup>\n                    ) on sheep were studied. Indeed, CA enzyme activities of Kangal sheep were investigated with using esterase procedure under\n                    <jats:italic>in vitro<\/jats:italic>\n                    conditions. The heavy metal concentrations inhibiting 50% of enzyme activity (IC\n                    <jats:sub>50<\/jats:sub>\n                    ) and\n                    <jats:italic>K<\/jats:italic>\n                    <jats:sub>i<\/jats:sub>\n                    values were obtained.\n                  <\/jats:p>","DOI":"10.1002\/jbt.22172","type":"journal-article","created":{"date-parts":[[2018,7,9]],"date-time":"2018-07-09T06:06:20Z","timestamp":1531116380000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Characterization and inhibition effects of some metal ions on carbonic anhydrase enzyme from Kangal Akkaraman sheep"],"prefix":"10.1002","volume":"32","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8710-2912","authenticated-orcid":false,"given":"Umit M.","family":"Kocyigit","sequence":"first","affiliation":[{"name":"Vocational School of Health Services Cumhuriyet University  Sivas 58140 Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3171-0633","authenticated-orcid":false,"given":"Parham","family":"Taslimi","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Faculty of Science Atat\u00fcrk University  Erzurum 25240 Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5993-1668","authenticated-orcid":false,"given":"\u0130lhami","family":"Gul\u00e7in","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Faculty of Science Atat\u00fcrk University  Erzurum 25240 Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,7,9]]},"reference":[{"key":"e_1_2_7_2_1","first-page":"969","volume-title":"Sheep Breeding (In Turkish)","author":"Akcapnar H.","year":"2000"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.smallrumres.2005.08.013"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.3109\/14756366.2015.1014476"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.3109\/14756366.2015.1054820"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.3109\/14756366.2016.1149479"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.3109\/14756366.2016.1156104"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.3109\/14756366.2016.1160077"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.3109\/14756366.2016.1170014"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1080\/14756366.2016.1189422"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1080\/14756366.2016.1198901"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/ardp.201600183"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1080\/14756366.2016.1238367"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1080\/14756366.2016.1244533"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.2174\/1570180814666161128120612"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bioorg.2017.03.001"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbt.21897"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbt.21931"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbt.21938"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bioorg.2017.07.010"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbt.21971"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbt.21972"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbt.21973"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbt.21930"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4615-2664-3_10"},{"key":"e_1_2_7_26_1","first-page":"121","volume-title":"Applied Research Ethics National Association","author":"Arena L.","year":"2002"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bmc.2009.01.067"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bmc.2008.09.028"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pestbp.2009.12.006"},{"key":"e_1_2_7_30_1","first-page":"580","volume":"59","author":"Ekinci D.","year":"2007","journal-title":"Pharmacol. 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