{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T23:19:31Z","timestamp":1790291971492,"version":"4.1.0"},"reference-count":45,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2018,7,5]],"date-time":"2018-07-05T00:00:00Z","timestamp":1530748800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100008552","name":"Haryana State Council for Science and Technology","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100008552","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001501","name":"University Grants Commission","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001501","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100009395","name":"Kurukshetra University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100009395","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Biochem &amp; Molecular Tox"],"published-print":{"date-parts":[[2018,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Toxicological studies have demonstrated the relation between use of agrochemicals and fertility issues within males. Thus, the present study aimed to elucidate the propensity of cypermethrin (CYP) in bringing testicular germ cell apoptosis and effective attenuation by vitamins C and E in caprines. Reproductive toxicity of CYP was evaluated using histomorphological, cytological, and biochemical changes in the testicular germ cells in dose\u2010dependent (1, 5, 10\u00a0\u03bcg\/mL) and time\u2010dependent (4, 6, 8 h) manner. Histological and ethidium bromide\/acridine orange fluorescence staining exhibited that vitamins C and E (0.5 and 1.0\u00a0mM) successfully diminished the CYP\u2010induced testicular germ cells apoptosis. CYP exposure along with vitamins C and E supplementation also resulted in significantly increased ferric reducing antioxidant power activity along with the antioxidant enzymes, namely catalase, superoxide dismutase, and glutathione\u2010s\u2010transferase, and decreased lipid peroxidation in testicular germ cells. Thus, vitamins C and E ameliorated CYP\u2010induced testicular germ cell apoptosis, thereby preventing spermatogonial cells degeneration and male infertility.<\/jats:p>","DOI":"10.1002\/jbt.22174","type":"journal-article","created":{"date-parts":[[2018,7,5]],"date-time":"2018-07-05T15:00:13Z","timestamp":1530802813000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":33,"title":["Antiapoptotic effects of vitamins C and E against cypermethrin\u2010induced oxidative stress and spermatogonial germ cell apoptosis"],"prefix":"10.1002","volume":"32","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2183-3058","authenticated-orcid":false,"given":"Jitender Kumar","family":"Bhardwaj","sequence":"first","affiliation":[{"name":"Reproductive Physiology Laboratory Department of Zoology Kurukshetra University Kurukshetra 136 119 India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Priya","family":"Kumari","sequence":"additional","affiliation":[{"name":"Reproductive Physiology Laboratory Department of Zoology Kurukshetra University Kurukshetra 136 119 India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Priyanka","family":"Saraf","sequence":"additional","affiliation":[{"name":"Reproductive Physiology Laboratory Department of Zoology Kurukshetra University Kurukshetra 136 119 India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abhay Singh","family":"Yadav","sequence":"additional","affiliation":[{"name":"Reproductive Physiology Laboratory Department of Zoology Kurukshetra University Kurukshetra 136 119 India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,7,5]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.reprotox.2008.05.060"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1017\/S1431927614013452"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tiv.2015.09.026"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/tox.22195"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/tox.20566"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/0165-1218(95)90007-1"},{"key":"e_1_2_7_8_1","first-page":"233","volume":"44","author":"Suman G.","year":"2006","journal-title":"Indian J. Exp. Biol."},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s002440010280"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chemosphere.2010.09.072"},{"key":"e_1_2_7_11_1","first-page":"141","volume":"10","author":"Abdollahi M.","year":"2010","journal-title":"Med. Sci. Monit"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00316637"},{"key":"e_1_2_7_13_1","doi-asserted-by":"crossref","first-page":"855","DOI":"10.1016\/S0022-3565(24)36686-8","volume":"281","author":"Gassner B.","year":"1997","journal-title":"J. Pharmacol. Exp. Ther."},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-4274(98)00399-3"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fct.2012.07.060"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pestbp.2006.11.007"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tox.2004.05.010"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00204-004-0593-9"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/mnfr.200400049"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pestbp.2006.06.010"},{"key":"e_1_2_7_21_1","unstructured":"Basic Animal Husbandry Statistics Ministry of Agriculture and Farmers Welfare. Government of India (GOI) New Delhi India 2014."},{"key":"e_1_2_7_22_1","volume-title":"Histochemistry, Theoretical and Applied","author":"Pearse A. G. E.","year":"1968"},{"key":"e_1_2_7_23_1","volume":"3","author":"Kasibhatla S.","year":"2006","journal-title":"Cold Spring Harb Protoc."},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0076-6879(84)05016-3"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)52451-6"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1974.tb03714.x"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)42083-8"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0076-6879(78)52032-6"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1006\/abio.1996.0292"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1002\/jat.1769"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1745-7262.2008.00405.x"},{"key":"e_1_2_7_32_1","first-page":"1348","volume":"10","author":"Funkhouser C. M.","year":"2013","journal-title":"PNAS"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.02-0752rev"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod57.6.1413"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1054\/plef.1999.0085"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-4274(00)00277-0"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.111.095224"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbt.20262"},{"key":"e_1_2_7_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tips.2010.12.002"},{"key":"e_1_2_7_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0304-3835(02)00203-3"},{"key":"e_1_2_7_41_1","doi-asserted-by":"publisher","DOI":"10.3109\/10409239509083491"},{"key":"e_1_2_7_42_1","doi-asserted-by":"publisher","DOI":"10.1155\/2012\/137676"},{"key":"e_1_2_7_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-4274(01)00427-1"},{"key":"e_1_2_7_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0955-2863(01)00160-7"},{"key":"e_1_2_7_45_1","doi-asserted-by":"publisher","DOI":"10.1159\/000417532"},{"key":"e_1_2_7_46_1","doi-asserted-by":"publisher","DOI":"10.1006\/taap.1996.8091"}],"container-title":["Journal of Biochemical and Molecular Toxicology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjbt.22174","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jbt.22174","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,7,5]],"date-time":"2025-07-05T15:57:53Z","timestamp":1751731073000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jbt.22174"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,7,5]]},"references-count":45,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2018,8]]}},"alternative-id":["10.1002\/jbt.22174"],"URL":"https:\/\/doi.org\/10.1002\/jbt.22174","archive":["Portico"],"relation":{},"ISSN":["1095-6670","1099-0461"],"issn-type":[{"value":"1095-6670","type":"print"},{"value":"1099-0461","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,7,5]]},"assertion":[{"value":"2018-02-08","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-06-15","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-07-05","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e22174"}}