{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,21]],"date-time":"2026-02-21T09:18:46Z","timestamp":1771665526781,"version":"3.50.1"},"reference-count":29,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2018,7,10]],"date-time":"2018-07-10T00:00:00Z","timestamp":1531180800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["473125\/2012\u20109"],"award-info":[{"award-number":["473125\/2012\u20109"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["310624\/2012\u20106"],"award-info":[{"award-number":["310624\/2012\u20106"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004901","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de Minas Gerais","doi-asserted-by":"publisher","award":["APQ\u201001712\u201014"],"award-info":[{"award-number":["APQ\u201001712\u201014"]}],"id":[{"id":"10.13039\/501100004901","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004901","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de Minas Gerais","doi-asserted-by":"publisher","award":["PPM\u201000167\u201016"],"award-info":[{"award-number":["PPM\u201000167\u201016"]}],"id":[{"id":"10.13039\/501100004901","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Biochem &amp; Molecular Tox"],"published-print":{"date-parts":[[2018,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Acute renal failure is one of the most frequent effects observed after taking medicine. Such situations have been tardily discovered, given that existing methods for assessing toxicity are not predictive. In this light, the present work evaluated the effects of gentamicin, a form of nephrotoxic drug, on HK\u20102 and HEK\u2010293 cells. By using MTT (3\u2010(4,5\u2010dimethylthiazol\u20102\u2010yl)\u20102,5\u2010diphenyltetrazolium bromide) and flow cytometry, both cells demonstrated that cytotoxicity occurs in a dose\u2010dependent manner through the processes of apoptosis and cell necrosis. Gene expression analysis showed a relative increase of expression for genes related to cell processes and classic biomarkers, such as TP53, CASP3, CASP8, CASP9, ICAM\u20101, EXOC3, KIM\u20101, and CST3. A decrease in expression for genes BCL2L1 and EGF was observed. This study, therefore, indicates that, when the methods are used together, gene expression analysis is able to evaluate the nephrotoxic potential of a substance.<\/jats:p>","DOI":"10.1002\/jbt.22189","type":"journal-article","created":{"date-parts":[[2018,7,11]],"date-time":"2018-07-11T03:46:02Z","timestamp":1531280762000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["In vitro evaluation of biomarkers of nephrotoxicity through gene expression using gentamicin"],"prefix":"10.1002","volume":"32","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0112-0560","authenticated-orcid":false,"given":"Maria A. A.","family":"Campos","sequence":"first","affiliation":[{"name":"Laborat\u00f3rio de Toxicologia (LabTox), Departamento de An\u00e1lises Cl\u00ednicas e Toxicol\u00f3gicas Faculdade de Farm\u00e1cia Universidade Federal de Minas Gerais  Belo Horizonte Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1886-5080","authenticated-orcid":false,"given":"Leonardo A.","family":"de Almeida","sequence":"additional","affiliation":[{"name":"Departamento de Microbiologia e Imunologia Universidade Federal de Alfenas  Alfenas Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7834-8475","authenticated-orcid":false,"given":"Marina F.","family":"Grossi","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Toxicologia (LabTox), Departamento de An\u00e1lises Cl\u00ednicas e Toxicol\u00f3gicas Faculdade de Farm\u00e1cia Universidade Federal de Minas Gerais  Belo Horizonte Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1049-6754","authenticated-orcid":false,"given":"Carlos A.","family":"Tagliati","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Toxicologia (LabTox), Departamento de An\u00e1lises Cl\u00ednicas e Toxicol\u00f3gicas Faculdade de Farm\u00e1cia Universidade Federal de Minas Gerais  Belo Horizonte Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,7,10]]},"reference":[{"key":"e_1_2_6_2_1","first-page":"34","volume":"404","author":"Kumar S.","year":"2014","journal-title":"Semin. Nephrol."},{"key":"e_1_2_6_3_1","first-page":"13","volume":"615","author":"Fuchs, T. C.","year":"2011","journal-title":"AAPS J."},{"key":"e_1_2_6_4_1","first-page":"15","volume":"112","author":"Dashti\u2010Khavidaki S.","year":"2012","journal-title":"J. Pharm. Pharm. Sci."},{"key":"e_1_2_6_5_1","first-page":"3","volume":"1","author":"Huang J. X.","year":"2015","journal-title":"Pharmacol. Res. Persp."},{"key":"e_1_2_6_6_1","first-page":"31","volume":"3","author":"Mohamed Ali O. S.","year":"2017","journal-title":"J. Biochem. Mol. Toxicol."},{"key":"e_1_2_6_7_1","first-page":"4","volume":"22","author":"Gomes A.","year":"2016","journal-title":"Resbcal"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1093\/ilar.43.Suppl_1.S43"},{"key":"e_1_2_6_9_1","first-page":"140","volume":"247","author":"Kim S.","year":"2014","journal-title":"Toxicol. Sci."},{"key":"e_1_2_6_10_1","first-page":"246","volume":"91","author":"Wang E.\u2010J.","year":"2008","journal-title":"Toxicology"},{"key":"e_1_2_6_11_1","first-page":"75","volume":"3674","author":"Lamoureux F.","year":"2012","journal-title":"J. Proteomics"},{"key":"e_1_2_6_12_1","first-page":"434","volume":"527","author":"Lee K. E.","year":"2013","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"e_1_2_6_13_1","first-page":"13","volume":"11","author":"Servais H.","year":"2008","journal-title":"Apoptosis"},{"key":"e_1_2_6_14_1","first-page":"56","volume":"505","author":"Gildea J. J.","year":"2010","journal-title":"Hypertension"},{"key":"e_1_2_6_15_1","first-page":"88","volume":"448","author":"Gunness P.","year":"2010","journal-title":"Can. J. Physiol. Pharmacol."},{"key":"e_1_2_6_16_1","first-page":"65","volume":"55","author":"Mosmann T.","year":"1983","journal-title":"J. Immunol. Methods"},{"key":"e_1_2_6_17_1","first-page":"91","volume":"17","author":"Romero D.","year":"2000","journal-title":"Rev Toxicol."},{"key":"e_1_2_6_18_1","first-page":"17","volume":"93","author":"Dyshlovoy S. A.","year":"2017","journal-title":"BMC Cancer"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms18030531"},{"key":"e_1_2_6_20_1","first-page":"27","volume":"435","author":"Silva S. C. T.","year":"2017","journal-title":"Toxicol. Mech. Methods"},{"key":"e_1_2_6_21_1","first-page":"574","volume":"193","author":"Stepanenko A. A.","year":"2015","journal-title":"Gene"},{"key":"e_1_2_6_22_1","first-page":"37","volume":"723","author":"Kondo C.","year":"2012","journal-title":"J. Toxicol. Sci."},{"key":"e_1_2_6_23_1","first-page":"21","volume":"624","author":"Bledsoe G.","year":"2006","journal-title":"Nephrol., Dial., Transplant."},{"key":"e_1_2_6_24_1","first-page":"15","volume":"348","author":"Bae E. H.","year":"2014","journal-title":"JRAAS"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.4161\/15592294.2014.990780"},{"key":"e_1_2_6_26_1","first-page":"285","volume":"610","author":"Ramesh G.","year":"2013","journal-title":"Am. J. Physiol.: Renal Physiol."},{"key":"e_1_2_6_27_1","first-page":"62","volume":"555","author":"Ozaki N.","year":"2010","journal-title":"Exp. Toxicol. Pathol."},{"key":"e_1_2_6_28_1","first-page":"35","volume":"258","author":"Tagliati C. A.","year":"2012","journal-title":"Drug Chem Toxicol."},{"key":"e_1_2_6_29_1","first-page":"301","volume":"171","author":"Zhang H.","year":"2011","journal-title":"Am. J. Physiol.\u2010Renal Physiol."},{"key":"e_1_2_6_30_1","first-page":"36","volume":"753","author":"Perco P.","year":"2006","journal-title":"Eur. J. Clin. Invest."}],"container-title":["Journal of Biochemical and Molecular Toxicology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjbt.22189","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jbt.22189","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,17]],"date-time":"2023-09-17T09:34:49Z","timestamp":1694943289000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jbt.22189"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,7,10]]},"references-count":29,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2018,9]]}},"alternative-id":["10.1002\/jbt.22189"],"URL":"https:\/\/doi.org\/10.1002\/jbt.22189","archive":["Portico"],"relation":{},"ISSN":["1095-6670","1099-0461"],"issn-type":[{"value":"1095-6670","type":"print"},{"value":"1099-0461","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,7,10]]},"assertion":[{"value":"2018-04-15","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-06-25","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-07-10","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e22189"}}