{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,28]],"date-time":"2026-07-28T10:21:39Z","timestamp":1785234099832,"version":"3.55.0"},"reference-count":31,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2018,7,17]],"date-time":"2018-07-17T00:00:00Z","timestamp":1531785600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004951","name":"Atat\u00fcrk \u00dcniversitesi","doi-asserted-by":"publisher","award":["2015\/113"],"award-info":[{"award-number":["2015\/113"]}],"id":[{"id":"10.13039\/501100004951","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Biochem &amp; Molecular Tox"],"published-print":{"date-parts":[[2018,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Glutathione\u2010<jats:italic>S<\/jats:italic>\u2010transferases (GSTs) have a function in xenobiotic metabolism. They are a significant multifunctional family with a wide variety of catalytic activities. In the current study, we determined <jats:italic>in vitro<\/jats:italic> inhibition effects of 2,4\u2010dichlorophenoxyacetic acid dimethylamine salt (2,4\u2010D DMA), haloxyfop\u2010<jats:italic>P<\/jats:italic>\u2010methyl, glyphosate isopropylamine, dichlorvos, and \u03bb\u2010cyhalothrin on purified GST. For this purpose, GST were purified from Van Lake fish <jats:italic>(Chalcalburnus tarichii<\/jats:italic> Pallas<jats:italic>)<\/jats:italic> liver with 29.25 EU\u00a0mg<jats:sup>\u22121<\/jats:sup> specific activity and 10.76% yield using GSH\u2013agarose affinity chromatographic method. The pesticides were tested at various concentrations on in vitro GST activity. <jats:italic>K<\/jats:italic><jats:sub>i<\/jats:sub> constants were calculated as 0.17\u00a0\u00b1\u00a00.01, 0.25\u00a0\u00b1\u00a00.05, 3.72\u00a0\u00b1\u00a00.32, 0.42\u00a0\u00b1\u00a00.06, and 0.025\u00a0\u00b1\u00a00.004\u00a0mM, for 2,4\u2010D DMA, haloxyfop\u2010<jats:italic>P<\/jats:italic>\u2010methyl, glyphosate isopropylamine, dichlorvos, and \u03bb\u2010cyhalothrin, respectively. \u03bb\u2010Cyhalothrin showed a better inhibitory effect compared to the other pesticides. The inhibition mechanisms of \u03bb\u2010cyhalothrin were competitive, while the other pesticides were noncompetitive.<\/jats:p>","DOI":"10.1002\/jbt.22196","type":"journal-article","created":{"date-parts":[[2018,7,17]],"date-time":"2018-07-17T14:21:29Z","timestamp":1531837289000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":60,"title":["Inhibition effects of pesticides on glutathione\u2010<i>S<\/i>\u2010transferase enzyme activity of Van Lake fish liver"],"prefix":"10.1002","volume":"32","author":[{"given":"Muhammet Serhat","family":"\u00d6zaslan","sequence":"first","affiliation":[{"name":"Department of Chemistry, Faculty of Sciences Atat\u00fcrk University  25240 Erzurum Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yeliz","family":"Demir","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Faculty of Sciences Atat\u00fcrk University  25240 Erzurum Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2430-8769","authenticated-orcid":false,"given":"Mine","family":"Aksoy","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Faculty of Sciences Atat\u00fcrk University  25240 Erzurum Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1877-3154","authenticated-orcid":false,"given":"\u00d6mer Irfan","family":"K\u00fcfrevio\u011flu","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Faculty of Sciences Atat\u00fcrk University  25240 Erzurum Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3667-6902","authenticated-orcid":false,"given":"\u015e\u00fckr\u00fc","family":"Beydemir","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Faculty of Pharmacy Anadolu University  26470 Eski\u015fehir Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,7,17]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpba.2017.08.011"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00128-006-0914-1"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.2478\/10004-1254-65-2014-2547"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.marpolbul.2011.11.026"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbt.21889"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbpb.2016.02.006"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj3600001"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.3109\/13813455.2015.1026912"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.pharmtox.45.120403.095857"},{"key":"e_1_2_7_11_1","author":"T\u00fcrkan F.","year":"2018","journal-title":"Arch Physiol Biochem"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.etap.2008.03.010"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.15666\/aeer\/1402_253264"},{"key":"e_1_2_7_14_1","first-page":"515","volume":"44","author":"Demir Y.","year":"2016","journal-title":"Hacettepe J. 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