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Snake venom metalloproteinases are responsible for local tissue damage and hemorrhage at the bitten site in viper envenomation, and this has led to a persistent search for metalloproteinase inhibitors. Here, we report the inhibitory effects of ascorbic acid against metalloproteinase from <jats:italic>Echis carinatus<\/jats:italic> venom both in\u2010silico and in\u2010vitro. Ascorbic acid effectively inhibited the proteolytic activity of <jats:italic>E. carinatus<\/jats:italic> venom in a dose\u2010dependent manner. Interaction studies of ascorbic acid with purified ecarin using isothermal titration calorimetry showed favorable binding energy and energetics. The molecular docking of ascorbic acid with ecarin revealed important interactions with residues at the active site pocket of ecarin. It was observed that the ligand behaves as a chelating inhibitor. Thus, the backbone structural scaffold of ascorbic acid can find potential use as building blocks in designing drug\u2010like molecules for viper bite management.<\/jats:p>","DOI":"10.1002\/jbt.22224","type":"journal-article","created":{"date-parts":[[2018,10,2]],"date-time":"2018-10-02T00:50:09Z","timestamp":1538441409000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Inhibitory effects of ascorbic acid toward snake venom metalloproteinase (SVMP) from Indian <i>Echis carinatus<\/i> venom: Insights from molecular modeling and binding studies"],"prefix":"10.1002","volume":"32","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6108-9040","authenticated-orcid":false,"given":"Manisha","family":"Choudhury","sequence":"first","affiliation":[{"name":"Centre of Advanced Studies in Crystallography and Biophysics, University of Madras, Guindy Campus Chennai Tamil Nadu India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vajravijayan","family":"Senthilvadivel","sequence":"additional","affiliation":[{"name":"Centre of Advanced Studies in Crystallography and Biophysics, University of Madras, Guindy Campus Chennai Tamil Nadu India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Devadasan","family":"Velmurugan","sequence":"additional","affiliation":[{"name":"Centre of Advanced Studies in Crystallography and Biophysics, University of Madras, Guindy Campus Chennai Tamil Nadu India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,10]]},"reference":[{"issue":"1","key":"e_1_2_6_1_2_1","first-page":"1","volume":"30","author":"Warrell D. 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