{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T10:49:44Z","timestamp":1790333384397,"version":"4.1.0"},"reference-count":33,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2018,12,31]],"date-time":"2018-12-31T00:00:00Z","timestamp":1546214400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100008323","name":"Zanjan University of Medical Sciences","doi-asserted-by":"publisher","award":["A\u201012\u2010769\u201013,14,15"],"award-info":[{"award-number":["A\u201012\u2010769\u201013,14,15"]}],"id":[{"id":"10.13039\/501100008323","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Biochem &amp; Molecular Tox"],"published-print":{"date-parts":[[2019,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Tungstate (W) is recognized as an agent of environmental pollution and a substitute to depleted uranium. According to some preliminary studies, tungstate toxicity is related to the formation of reactive oxygen species (ROS) under abnormal pathological conditions. The kidneys and liver are the main tungstate accumulation sites and important targets of tungstate toxicity. Since the mitochondrion is the main ROS production site, we evaluated the mechanistic toxicity of tungstate in isolated mitochondria for the first time, following a two\u2010step ultracentrifugation method. Our findings demonstrated that tungstate\u2010induced mitochondrial dysfunction is related to the increased formation of ROS, lipid peroxidation, and potential membrane collapse, correlated with the amelioration of adenosine triphosphate and glutathione contents. The present study indicated that mitochondrial dysfunction was associated with disruptive effects on the mitochondrial respiratory chain and opening of mitochondrial permeability transition (MPT) pores, which is correlated with cytochrome <jats:italic>c<\/jats:italic> release. Our findings suggest that high concentrations of tungstate (2\u2009mM)\u2010favored MPT pore opening in the inner membranes of liver and kidney mitochondria of rats. Besides, the results indicated higher tungstate susceptibility in the kidneys, compared with the liver.<\/jats:p>","DOI":"10.1002\/jbt.22266","type":"journal-article","created":{"date-parts":[[2018,12,31]],"date-time":"2018-12-31T22:42:58Z","timestamp":1546296178000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":26,"title":["High doses of sodium tungstate can promote mitochondrial dysfunction and oxidative stress in isolated mitochondria"],"prefix":"10.1002","volume":"33","author":[{"given":"Ghazale","family":"Cheraghi","sequence":"first","affiliation":[{"name":"Zanjan Applied Pharmacology Research Center, Zanjan University of Medical Sciences Zanjan Iran"},{"name":"Department of Pharmacology and Toxicology School of Pharmacy, Zanjan University of Medical Sciences Zanjan Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elnaz","family":"Hajiabedi","sequence":"additional","affiliation":[{"name":"Zanjan Applied Pharmacology Research Center, Zanjan University of Medical Sciences Zanjan Iran"},{"name":"Department of Pharmacology and Toxicology School of Pharmacy, Zanjan University of Medical Sciences Zanjan Iran"},{"name":"Food and Drug Administration Iran, University of Medical Sciences Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Behnaz","family":"Niaghi","sequence":"additional","affiliation":[{"name":"Zanjan Applied Pharmacology Research Center, Zanjan University of Medical Sciences Zanjan Iran"},{"name":"Department of Pharmacology and Toxicology School of Pharmacy, Zanjan University of Medical Sciences Zanjan Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Firouzeh","family":"Nazari","sequence":"additional","affiliation":[{"name":"Food and Drug Administration Iran, University of Medical Sciences Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Parvaneh","family":"Naserzadeh","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Toxicology Shahid Beheshti University of Medical Sciences Tehran Iran"},{"name":"Student Research Committee, School of Pharmacy Shahid Beheshti University of Medical Sciences Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6300-7398","authenticated-orcid":false,"given":"Mir\u2010Jamal","family":"Hosseini","sequence":"additional","affiliation":[{"name":"Zanjan Applied Pharmacology Research Center, Zanjan University of Medical Sciences Zanjan Iran"},{"name":"Department of Pharmacology and Toxicology School of Pharmacy, Zanjan University of Medical Sciences Zanjan Iran"},{"name":"Food and Drug Administration Iran, University of Medical Sciences Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,12,31]]},"reference":[{"key":"e_1_2_8_1_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fct.2015.05.003"},{"key":"e_1_2_8_1_3_1","doi-asserted-by":"publisher","DOI":"10.2174\/0929867053764635"},{"issue":"2","key":"e_1_2_8_1_4_1","first-page":"714","volume":"294","author":"Le Lamer S.","year":"2000","journal-title":"J. Pharmacol. Exp. Ther."},{"key":"e_1_2_8_1_5_1","unstructured":"Agency for Toxic Substances and Disease Registry (ATSDR).2005. Toxicological profile for tungsten (update). Atlanta GA: U.S. Department of Health and Human Services Public Health Service."},{"key":"e_1_2_8_1_6_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0077546"},{"key":"e_1_2_8_1_7_1","doi-asserted-by":"publisher","DOI":"10.1385\/BTER:88:2:097"},{"key":"e_1_2_8_1_8_1","doi-asserted-by":"publisher","DOI":"10.3109\/15376516.2013.787132"},{"key":"e_1_2_8_1_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.taap.2010.04.021"},{"issue":"1","key":"e_1_2_8_1_10_1","volume":"6","author":"Bertinat R.","year":"2015","journal-title":"J. Clin. Cell. Immunol."},{"key":"e_1_2_8_1_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12011-012-9350-8"},{"key":"e_1_2_8_1_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12011-009-8537-0"},{"key":"e_1_2_8_1_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jtemb.2014.01.004"},{"key":"e_1_2_8_1_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/tox.21900"},{"key":"e_1_2_8_1_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(76)90527-3"},{"key":"e_1_2_8_1_16_1","doi-asserted-by":"publisher","DOI":"10.3109\/01480545.2015.1092039"},{"key":"e_1_2_8_1_17_1","doi-asserted-by":"publisher","DOI":"10.1177\/0960327114565492"},{"key":"e_1_2_8_1_18_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1016039604958"},{"key":"e_1_2_8_1_19_1","doi-asserted-by":"publisher","DOI":"10.1080\/02772248.2014.884206"},{"key":"e_1_2_8_1_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejphar.2015.04.006"},{"key":"e_1_2_8_1_21_1","doi-asserted-by":"publisher","DOI":"10.3109\/15622975.2016.1149218"},{"key":"e_1_2_8_1_22_1","doi-asserted-by":"publisher","DOI":"10.4149\/gpb_2014020"},{"issue":"3","key":"e_1_2_8_1_23_1","first-page":"143","volume":"12","author":"Heidari Z.","year":"2008","journal-title":"Iran. Biomed. J."},{"issue":"3","key":"e_1_2_8_1_24_1","first-page":"340","volume":"10","author":"Uskokovi\u0107\u2010Markovi\u0107 S.","year":"2007","journal-title":"J. Pharm. Pharm. Sci."},{"key":"e_1_2_8_1_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbadis.2003.12.004"},{"key":"e_1_2_8_1_26_1","doi-asserted-by":"publisher","DOI":"10.1269\/jrr.40.101"},{"key":"e_1_2_8_1_27_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00204-008-0313-y"},{"issue":"2","key":"e_1_2_8_1_28_1","first-page":"675","volume":"13","author":"Shokrzadeh M.","year":"2014","journal-title":"Iran J. Pharm. Res."},{"key":"e_1_2_8_1_29_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-81-322-2035-0_2"},{"key":"e_1_2_8_1_30_1","doi-asserted-by":"publisher","DOI":"10.1080\/02772248.2011.643566"},{"key":"e_1_2_8_1_31_1","doi-asserted-by":"publisher","DOI":"10.4103\/0971-6580.172250"},{"issue":"2","key":"e_1_2_8_1_32_1","first-page":"495","volume":"11","author":"Daraie B.","year":"2012","journal-title":"Iran. J. Pharm. Res"},{"key":"e_1_2_8_1_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ecoenv.2010.05.005"},{"key":"e_1_2_8_1_34_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10863-009-9225-7"}],"container-title":["Journal of Biochemical and Molecular Toxicology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjbt.22266","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jbt.22266","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jbt.22266","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jbt.22266","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,10]],"date-time":"2023-09-10T18:22:14Z","timestamp":1694370134000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jbt.22266"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,12,31]]},"references-count":33,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2019,4]]}},"alternative-id":["10.1002\/jbt.22266"],"URL":"https:\/\/doi.org\/10.1002\/jbt.22266","archive":["Portico"],"relation":{},"ISSN":["1095-6670","1099-0461"],"issn-type":[{"value":"1095-6670","type":"print"},{"value":"1099-0461","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,12,31]]},"assertion":[{"value":"2018-06-05","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-10-24","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-12-31","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e22266"}}