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Induction of acute liver injury in mice was established by a single intraperitoneal (IP) injection of APAP (0.5\u2009g\/kg), then mice were injected with Irb (50 or 75\u2009mg\/kg, IP), each given twice at 1 and 12\u2009hours post APAP injection. Liver functions, hepatic oxidative and nitrosative stress markers, and liver histopathology were determined after 24\u2009hours. Hepatic cytochrome P450 2E1 (CYP2E1), nuclear factor\u2010\u03baB (NF\u2010\u03baB), tumor necrosis factor\u2010\u03b1 (TNF\u2010\u03b1), caspase\u20103, B\u2010cell lymphoma 2 (Bcl\u20102), and Bcl\u20102\u2010associated X protein (Bax) levels were also estimated. Immunohistochemical evaluations of hepatic expression of phosphorylated NF\u2010kB and active caspase\u20103 were assigned. Irb treatment attenuated APAP\u2010induced acute liver injury. Irb suppressed APAP\u2010caused elevation of liver enzymes as well as oxidative and nitrosative stress in liver tissues as evidenced by the decrease in hepatic CYP2E1 expression and hepatic levels of malondialdehyde and nitric oxide in addition to the elevated hepatic superoxide dismutase activity and reduced glutathione concentration. Also, Irb mitigated APAP\u2010induced inflammation in liver tissues via decreasing the expression of hepatic NF\u2010\u03baB, phosphorylated NF\u2010\u03baB and TNF\u2010\u03b1, and attenuated hepatic apoptosis via decreasing Bax\/Bcl\u20102 ratio and caspase 3 expression and activation. Also, Irb mitigated the APAP\u2010induced histopathological changes in liver specimens. These data suggested that Irb ameliorates APAP\u2010induced acute liver injury through antioxidant, anti\u2010inflammatory, and antiapoptotic activities.<\/jats:p>","DOI":"10.1002\/jbt.22447","type":"journal-article","created":{"date-parts":[[2020,1,22]],"date-time":"2020-01-22T16:31:35Z","timestamp":1579710695000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":31,"title":["Irbesartan mitigates acute liver injury, oxidative stress, and apoptosis induced by acetaminophen in mice"],"prefix":"10.1002","volume":"34","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5974-2899","authenticated-orcid":false,"given":"Manar G.","family":"Helal","sequence":"first","affiliation":[{"name":"Department of Pharmacology and Toxicology, Faculty of Pharmacy Mansoura University Mansoura Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yara A.","family":"Samra","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Faculty of Pharmacy Mansoura University Mansoura Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,1,22]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1472-8206.2008.00608.x"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.20948"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1742-7843.2005.pto_96613.x"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fct.2015.03.020"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1189\/jlb.0403152"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbagen.2011.09.018"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jss.2012.05.069"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms19051335"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phymed.2018.09.234"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1081\/CLT-100102453"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/0002-9343(91)90296-A"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMct0708278"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1080\/15563650802665587"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00011-011-0386-6"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.atherosclerosis.2007.12.052"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.2337\/diabetes.54.12.3442"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/oby.2007.114"},{"key":"e_1_2_8_19_1","first-page":"107","volume":"30","author":"Kato J.","year":"2012","journal-title":"Int. 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