{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T09:56:14Z","timestamp":1753869374161,"version":"3.41.2"},"reference-count":30,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2020,2,11]],"date-time":"2020-02-11T00:00:00Z","timestamp":1581379200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Biochem &amp; Molecular Tox"],"published-print":{"date-parts":[[2020,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Treatment with Lipitor is associated with several adverse impacts. Here we investigated the effects of low Lipitor nanoparticles (atorvastatin calcium nanopartilcle [AC\u2010NP]), with size less than 100\u2009, on enzymes of lipid metabolism and inflammation in cardiac, hepatic, and brain tissues of hypercholestremic adult male rats. Adult male rats were divided into five experimental groups. In group 1, the intact control (normal pellet diet), animals were fed a normal control diet; the other four groups were fed a high\u2010fat diet (HFD) for 6 weeks. After 6 weeks, groups from 2 to 5 were assigned as a positive control (HFD), HFD\u2009+\u2009Lipitor, HFD\u2009+\u2009AC\u2010NP\u2010R1, or HFD\u2009+\u2009AC\u2010NP\u2010R2. Different treatments were administrated orally for two regimen periods (R1 daily and R2 once every 3 days). The treatment was conducted for two consecutive weeks. The HFD group faced a significant elevation in 3\u2010hydroxy\u20103\u2010methylglutaryl coenzyme A (HMG\u2010CoA), associated with a significant reduction in low\u2010density lipoprotein receptor (LDL\u2010R) along with cholesterol 7 \u03b1\u2010hydroxylase enzyme in hepatic tissues, compared with the control group. Also, the HFD group induced hepatic, cardiac, and brain inflammation, evidenced by increased hepatic oxidative stress markers and cardiac homocysteine, together with elevated proinflammatory cytokines interleukin\u20101\u03b2 (IL\u20101\u03b2) and IL\u20106 levels in brain tissue, compared with the control group. Different AC\u2010NP treatments significantly augmented both mRNA LDL\u2010R and mRNA 7\u03b1\u2010hydroxylase expression in hepatic tissues, associated with significant depletion in mRNA HMG\u2010CoA expression, compared with HFD\u2009+\u2009Lipitor. The inflammation symptoms were ameliorated by the AC\u2010NP treatments, compared to HFD\u2009+\u2009Lipitor. Lipitor encapsulation in NP formulation results in increased efficiency and reduced dose\u2010related adverse effects known to be associated with the Lipitor chronic administration.<\/jats:p>","DOI":"10.1002\/jbt.22465","type":"journal-article","created":{"date-parts":[[2020,2,12]],"date-time":"2020-02-12T07:34:23Z","timestamp":1581492863000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["High\u2010fat diet induced alteration in lipid enzymes and inflammation in cardiac and brain tissues: Assessment of the effects of Atorvastatin\u2010loaded nanoparticles"],"prefix":"10.1002","volume":"34","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8589-1556","authenticated-orcid":false,"given":"Marwa M.","family":"Abd\u2010Rabo","sequence":"first","affiliation":[{"name":"Department of Hormone National Organization for Drug Control and Research Giza Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lobna F.","family":"Wahman","sequence":"additional","affiliation":[{"name":"Department of Hormone National Organization for Drug Control and Research Giza Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rania","family":"El Hosary","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutics National Organization for Drug Control and Research  Giza Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Iman S.","family":"Ahmed","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutics and Pharmaceutical Technology, College of Pharmacy University of Sharjah  Sharjah United Arab Emirates"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,2,11]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1742\u20107843.2009.00497.x"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.2174\/156802609789869628"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11883\u2010012\u20100307\u20103"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00125\u2010015\u20103525\u20108"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.2165\/00129784-200707010-00004"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2275(20)41615-3"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2275(20)34205-X"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1745\u20107254.2008.00855.x"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.3233\/JAD\u20102011\u2010110599"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/J.BBI.2011.02.008"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010017\u201011070\u2010x"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1592\/phco.23.15.1663.31953"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpharm.2016.03.045"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.molpharmaceut.7b00856"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1067\/mhj.2000.108245"},{"volume-title":"Theory and Practice of Histological Techniques","year":"1996","author":"Bancroft J. 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