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Moreover, heat shock protein 70 (Hsp70) can be used as a protective protein, and Hsp70 upregulated drastically when exposed to stressful conditions. We aimed to assess that MSCs overexpressed Hsp70 could enhance the capacity of MSCs and have a good therapeutic effect on phosgene\u2010induced ALI. We transduced MSCs with Hsp70 and then we tested the function of the transduced MSCs. Sprague Dawley rats inhaled phosgene in a closed container for 5\u2009minutes. The transduced MSCs and MSCs were administered via the trachea immediately. Rats in each group were killed at 6, 24, and 48\u2009hours after exposure. Compared to\u00a0MSCs, MSCs overexpressed Hsp70 enhanced MSCs viability, antiapoptotic ability, and migration ability, and these effects disappeared when using the phosphatidylinositol 3\u2011kinase\/protein kinase B (PI3K\/AKT) pathway inhibitor. Furthermore, the results of pathological alterations improved. The lung wet\u2010to\u2010dry ratio declined. The lung injury index total protein content and total cells in bronchoalveolar lavage fluid (BALF) also declined. The level of tumor necrosis factor \u03b1 declined and the level of interleukin\u201010 improved in BALF and serum. MSCs overexpressed Hsp70 can enhance the capacity and efficacy of MSCs in the treatment of phosgene\u2010induced ALI and may be mediated through the PI3k\/AKT signaling pathway. This article introduces a new approach to stem cell therapy for improving the efficacy of phosgene\u2010induced ALI.<\/jats:p>","DOI":"10.1002\/jbt.22515","type":"journal-article","created":{"date-parts":[[2020,5,12]],"date-time":"2020-05-12T07:55:45Z","timestamp":1589270145000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Overexpression of heat shock protein 70 enhanced mesenchymal stem cell\u00a0treatment efficacy in phosgene\u2010induced acute lung injury"],"prefix":"10.1002","volume":"34","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7123-7935","authenticated-orcid":false,"given":"Chaoyuan","family":"Jin","sequence":"first","affiliation":[{"name":"Center of Emergency and Intensive Care Unit, Jinshan Hospital Fudan University  Shanghai China"},{"name":"Medical Center of Chemical Injury, Jinshan Hospital Fudan University  Shanghai China"},{"name":"Medical Research Center for Chemical Injury, Emergency 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