{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,20]],"date-time":"2026-01-20T03:35:10Z","timestamp":1768880110650,"version":"3.49.0"},"reference-count":45,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2020,6,25]],"date-time":"2020-06-25T00:00:00Z","timestamp":1593043200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Biochem &amp; Molecular Tox"],"published-print":{"date-parts":[[2020,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Today, it has been proven that the nanoparticles such as superparamagnetic iron oxide nanoparticles (SPIONs) have widespread use in biomedical applications, for instance, in magnetic resonance imaging and targeted delivery of drugs. Despite many studies on SPIONs in diagnosing some diseases like cancer, it has not been investigated on the oral tongue squamous cell carcinoma (OTSCC) detection by the NPs. Hence, the present study has been designed to assess the in vitro cytotoxicity of SPIONs on the isolated mitochondria of OTSCC by mitochondrial tests. Isolated mitochondria were removed from the separated cancer and control tissues from the squamous cells of tango in male Wistar rats (6 or 8 weeks) and exposed to the different concentrations of SPIONs (30, 60, and 120\u2009nM). A rise in the production of reactive oxygen species is one of the significant mechanisms of this study, followed by a collapse of mitochondrial membrane potential, the escape of mitochondrial cytochrome <jats:italic>c<\/jats:italic>, and mitochondrial swelling in the exposed isolated mitochondria of OTSCC with SPIONs. Furthermore, our results indicated that the exposure to the SPIONs reduced the activity of succinate dehydrogenase in complex II of the mitochondria obtained from cancerous oral tongue squamous. So the SPIONs can induce selective cytotoxicity on the OTSCC mitochondria without significant effects on the control mitochondria. Based on the results and further studies about <jats:italic>in vivo<\/jats:italic> experiments in this regard, it is concluded the SPIONs may be a hopeful therapeutic candidate for the treatment of OTSCC.<\/jats:p>","DOI":"10.1002\/jbt.22557","type":"journal-article","created":{"date-parts":[[2020,6,25]],"date-time":"2020-06-25T11:40:54Z","timestamp":1593085254000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["Selective anticancer activity of superparamagnetic iron oxide nanoparticles (SPIONs) against oral tongue cancer using in vitro methods: The key role of oxidative stress on cancerous mitochondria"],"prefix":"10.1002","volume":"34","author":[{"given":"Jahanfar","family":"Jahanbani","sequence":"first","affiliation":[{"name":"Department of Oral &amp; Maxillofacial Pathology, Faculty of Dentistry, Tehran Medical Sciences Islamic Azad University  Tehran Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maryam","family":"Ghotbi","sequence":"additional","affiliation":[{"name":"Department of Oral &amp; Maxillofacial Pathology, Faculty of Dentistry, Tehran Medical Sciences Islamic Azad University  Tehran Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fatemeh","family":"Shahsavari","sequence":"additional","affiliation":[{"name":"Department of Oral &amp; Maxillofacial Pathology, Faculty of Dentistry, Tehran Medical Sciences Islamic Azad University  Tehran Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Enayatollah","family":"Seydi","sequence":"additional","affiliation":[{"name":"Department of Occupational Health and Safety Engineering, School of Health Alborz University of Medical Sciences  Karaj Iran"},{"name":"Research Center for Health, Safety and Environment Alborz University of Medical Sciences  Karaj Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shabnam","family":"Rahimi","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Toxicology, Faculty of Pharmacy Shahid Beheshti University of Medical Sciences  Tehran Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4555-4168","authenticated-orcid":false,"given":"Jalal","family":"Pourahmad","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Toxicology, Faculty of Pharmacy Shahid Beheshti University of Medical Sciences  Tehran Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,6,25]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/hed.24589"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2010.31.7883"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.oraloncology.2010.06.008"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.23033"},{"key":"e_1_2_9_6_1","first-page":"696","volume":"15","author":"Hashibe M.","year":"2006","journal-title":"Biomarkers"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1093\/jnci\/djk179"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1470-2045(09)70326-2"},{"key":"e_1_2_9_9_1","first-page":"541","volume":"18","author":"Hashibe M.","year":"2009","journal-title":"Biomarkers"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/hed.21567"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1503\/cmaj.050623"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/cncr.26229"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1080\/02841860310010907"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joms.2008.04.009"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.25358"},{"key":"e_1_2_9_16_1","first-page":"275","volume":"1","author":"Yan W.","year":"2011","journal-title":"Am. J. Cancer Res."},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2407-10-166"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/cbic.200900017"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2019.03.027"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-018-07882-8"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mito.2004.07.027"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.2174\/1871520616666160425110839"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbpa.2008.06.002"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.2174\/138945012802009044"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(76)90527-3"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022-1759(83)90303-4"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1177\/0960327110372643"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0005-2728(03)00110-5"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jinorgbio.2009.11.007"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbt.22303"},{"key":"e_1_2_9_31_1","doi-asserted-by":"crossref","first-page":"1311","DOI":"10.1002\/cmdc.200800091","volume":"3","author":"Wang A. Z.","year":"2008","journal-title":"ChemMedChem: Chemistry Enabling Drug Discovery"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.3402\/nano.v1i0.5358"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1039\/C5NR02718G"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1369-7021(11)70163-8"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mattod.2015.08.022"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pnsc.2016.09.004"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1002\/tox.22262"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1006\/abbi.1993.1311"},{"key":"e_1_2_9_39_1","doi-asserted-by":"crossref","unstructured":"B. G. J.Halliwell Free radicals in biology and medicine;2007.","DOI":"10.1002\/3527600906.mcb.200300036"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt1201-1141"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.addr.2006.09.013"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.7150\/thno.3462"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejrad.2007.02.010"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbm.a.32972"},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tiv.2013.09.006"},{"key":"e_1_2_9_46_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12951-015-0073-9"}],"container-title":["Journal of Biochemical and Molecular Toxicology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjbt.22557","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jbt.22557","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jbt.22557","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jbt.22557","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,25]],"date-time":"2023-09-25T08:42:59Z","timestamp":1695631379000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jbt.22557"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,6,25]]},"references-count":45,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2020,10]]}},"alternative-id":["10.1002\/jbt.22557"],"URL":"https:\/\/doi.org\/10.1002\/jbt.22557","archive":["Portico"],"relation":{},"ISSN":["1095-6670","1099-0461"],"issn-type":[{"value":"1095-6670","type":"print"},{"value":"1099-0461","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,6,25]]},"assertion":[{"value":"2020-01-10","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-06-05","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-06-25","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e22557"}}