{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T14:44:39Z","timestamp":1786977879602,"version":"build-2736575974"},"reference-count":60,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2020,8,26]],"date-time":"2020-08-26T00:00:00Z","timestamp":1598400000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Biochem &amp; Molecular Tox"],"published-print":{"date-parts":[[2020,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Vitamin U (Vit U) is a novel free\u2010radical scavenger. The protective effect of Vit U on valproic acid (VPA)\u2010induced lung damage was examined. Rats were divided into four groups: control rats; rats given Vit U (50\u2009mg\/kg\/d, by gavage) for 15 days; rats treated with VPA (500\u2009mg\/kg\/d, intraperitoneally) for 15 days; and rats were given VPA\u2009+\u2009Vit U (in same dose and time). On the 16th day of the experiment, the lungs were collected from rats. Lung structure, pulmonary oxidant\/antioxidant parameters and Nrf2, \u03b1\u2010SMA, and collagen\u20101 were evaluated by microscopic and biochemical analysis. Additionally, it was determined the interactions of Vit U with Nrf2 and Keap1 by in silico analysis. VPA administration increased lipid peroxidation and the activity of lactate dehydrogenase and myeloperoxidase. However, it decreased the glutathione level, and the activities of glutathione peroxidase, glutathione\u2010\n                    <jats:italic>S<\/jats:italic>\n                    \u2010transferase, catalase, and superoxide dismutase. VPA\u2010mediated oxidative stress prompted structural distortion and fibrotic alterations in the lung. Vit U supplementation reversed structural and biochemical alterations, induced antioxidant system through Nrf2 activation, and attenuated fibrosis by reducing collagen expression in VPA\u2010administered rats. However, Vit U pretreatment was unable to reduce \u03b1\u2010SMA levels in the lung of VPA\u2010treated rats. Molecular docking analysis showed the binding of Vit U to ETGE motif leads to dissociation of Nrf2 from the Nrf2\/Keap1 complex and its transfer to nuclei. In conclusion, Vit U attenuated VPA\u2010induced tissue damage by restoring antioxidative systems through amelioration of Nrf2 activity in the lung under oxidative stress.\n                  <\/jats:p>","DOI":"10.1002\/jbt.22602","type":"journal-article","created":{"date-parts":[[2020,8,26]],"date-time":"2020-08-26T15:05:18Z","timestamp":1598454318000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":29,"title":["The protective effect of vitamin U on valproic acid\u2010induced lung toxicity in rats via amelioration of oxidative stress"],"prefix":"10.1002","volume":"34","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8515-8338","authenticated-orcid":false,"given":"Fusun","family":"Oztay","sequence":"first","affiliation":[{"name":"Department of Biology, Faculty of Science Istanbul University Vezneciler Istanbul Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3363-1290","authenticated-orcid":false,"given":"Sevim","family":"Tunali","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Faculty of Engineering Istanbul University\u2014Cerrahpasa Avcilar Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9107-2381","authenticated-orcid":false,"given":"Ozgecan","family":"Kayalar","sequence":"additional","affiliation":[{"name":"Department of Biology, Faculty of Science Istanbul University Vezneciler Istanbul Turkey"},{"name":"Koc University School of Medicine Koc University Research Center for Translational Medicine (KUTTAM) Istanbul Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4185-4363","authenticated-orcid":false,"given":"Refiye","family":"Yanardag","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Faculty of Engineering Istanbul University\u2014Cerrahpasa Avcilar Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,8,26]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"crossref","unstructured":"Y.Li Q.Zhang D.Qi L.Zhang L.Yi Q.Li Z.Zhang J. HeadachePain2016 17 49.","DOI":"10.1186\/s10194-016-0631-z"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/febs.15122"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.brainresbull.2009.09.003"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0048791"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12964-020-00585-y"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jsbmb.2020.105723"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.clinbiochem.2013.06.012"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jphs.2020.01.007"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1468-1331.2011.03409.x"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.4103\/0970-2113.152646"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/appy.12276"},{"issue":"1","key":"e_1_2_9_13_1","first-page":"39","volume":"84","author":"Cheney G.","year":"1956","journal-title":"Calif. Med."},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1136\/gut.39.1.77"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00210-017-1447-3"},{"issue":"1","key":"e_1_2_9_16_1","first-page":"1","volume":"75","author":"Sancar Bas S.","year":"2016","journal-title":"IUFS J. Biol."},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1177\/0960327114561665"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1124\/jpet.116.237503"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.redox.2013.08.006"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.03-1127fje"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcc.20084"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-019-43320-5"},{"key":"e_1_2_9_23_1","first-page":"112","volume-title":"A Manual Biochemical Methods","author":"Beutler E.","year":"1975"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/0009-8981(86)90247-0"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0076-6879(84)05016-3"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/0041-008X(86)90286-3"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0076-6879(81)77046-0"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0076-6879(81)77053-8"},{"issue":"16","key":"e_1_2_9_29_1","first-page":"4443","volume":"51","author":"Wei H.","year":"1991","journal-title":"Cancer Res."},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/0002-8703(57)90149-7"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)52451-6"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10545-008-0841-x"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1097\/MAJ.0b013e31805339ae"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.4046\/trd.2014.77.3.145"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.112.103176"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10753-013-9686-z"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1158\/1940-6207.CAPR-13-0254"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1093\/toxsci\/kfi184"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1006\/phrs.1999.0603"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/0300-483X(96)03352-5"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-2007-973560"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1089\/ars.2007.1927"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.1177\/0748233715576615"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.1177\/0960327114554047"},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00709-015-0796-3"},{"issue":"2","key":"e_1_2_9_46_1","first-page":"166","volume":"45","author":"Emekli\u2010Alturfan E.","year":"2015","journal-title":"Ann. Clin. Lab. Sci."},{"key":"e_1_2_9_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fct.2012.07.056"},{"key":"e_1_2_9_48_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.pharmtox.46.120604.141046"},{"key":"e_1_2_9_49_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2005.08.043"},{"key":"e_1_2_9_50_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2008.10.040"},{"key":"e_1_2_9_51_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20050538"},{"key":"e_1_2_9_52_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi00024a013"},{"key":"e_1_2_9_53_1","doi-asserted-by":"publisher","DOI":"10.3109\/13880209.2014.997252"},{"key":"e_1_2_9_54_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pupt.2013.03.003"},{"key":"e_1_2_9_55_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12011-014-0142-1"},{"key":"e_1_2_9_56_1","doi-asserted-by":"publisher","DOI":"10.3109\/08977194.2015.1109511"},{"key":"e_1_2_9_57_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0182809"},{"key":"e_1_2_9_58_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamcr.2016.09.012"},{"key":"e_1_2_9_59_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M114.573519"},{"key":"e_1_2_9_60_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pupt.2005.02.001"},{"key":"e_1_2_9_61_1","doi-asserted-by":"publisher","DOI":"10.3892\/mmr.2017.6546"}],"container-title":["Journal of Biochemical and Molecular Toxicology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jbt.22602","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jbt.22602","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jbt.22602","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,3]],"date-time":"2023-09-03T08:30:51Z","timestamp":1693729851000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jbt.22602"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,8,26]]},"references-count":60,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2020,12]]}},"alternative-id":["10.1002\/jbt.22602"],"URL":"https:\/\/doi.org\/10.1002\/jbt.22602","archive":["Portico"],"relation":{},"ISSN":["1095-6670","1099-0461"],"issn-type":[{"value":"1095-6670","type":"print"},{"value":"1099-0461","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,8,26]]},"assertion":[{"value":"2020-05-19","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-08-10","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-08-26","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e22602"}}