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Recently, polydeoxyribonucleotide (PDRN) has been shown to have anti\u2010inflammatory and regenerative effects in various tissues. However, the bronchial\u2010related mechanism is not well\u2010understood. Hence, this experiment is intended to demonstrate the beneficial effect of PDRN administration on PM10\u2010induced injury in human bronchial\u2010derived NCI\u2010H358 cells. To confirm the protective effect of PDRN, PM10 was applied after PDRN pretreatment to confirm changes in NCI\u2010H358 cells. Experiments were conducted to measure cell survival, cytotoxicity, inflammation, and apoptotic factor changes. WST\u20108 assay was used to confirm cell viability, and lactate dehydrogenase assay was used to obtain cytotoxicity. In addition, changes in inflammatory cytokines and apoptotic factors were confirmed by enzyme\u2010linked immunosorbent assay and Western blot. Decreased cell viability and increased cytotoxicity, inflammatory cytokines, and apoptotic factors were observed after exposure to PM10. However, pretreatment with PDRN enhanced cell viability and reduced cytotoxicity. In addition, the expression of inflammatory cytokines such as tumor necrosis factor\u2010\u03b1, interleukin\u20106 (IL\u20106), and IL\u20101\u03b2, and cell death factors such as Apaf\u20101, cyt c, caspase\u20103, caspase\u20109, Bid, and Bax\/Bcl\u20102 ratio were decreased by PDRN administration in PM10\u2010exposed NCI\u2010H358 cells. PDRN, an A2AR agonist, affects cAMP activation and regulation of phosphorylation of PKA and CREB. In addition, treatment with A2AR antagonist 3,7\u2010dimethyl\u20101\u2010propargylxanthine significantly blocked PDRN's effect. These anti\u2010cytotoxicity, anti\u2010inflammation, and anti\u2010apoptosis effects of PDRN can be attributed to the adenosine A2AR enhancing effect on PM10\u2010exposed bronchial cells.<\/jats:p>","DOI":"10.1002\/jbt.22635","type":"journal-article","created":{"date-parts":[[2020,9,28]],"date-time":"2020-09-28T12:55:38Z","timestamp":1601297738000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["Attenuation effect of polydeoxyribonucleotide on inflammatory cytokines and apoptotic factors induced by particulate matter (PM10) damage in human bronchial cells"],"prefix":"10.1002","volume":"35","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5173-1258","authenticated-orcid":false,"given":"Lakkyong","family":"Hwang","sequence":"first","affiliation":[{"name":"Department of Physiology, College of Medicine Kyung Hee University  Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2696-6869","authenticated-orcid":false,"given":"Il\u2010Gyu","family":"Ko","sequence":"additional","affiliation":[{"name":"Department of Physiology, College of Medicine Kyung Hee University  Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5594-5915","authenticated-orcid":false,"given":"Jun\u2010Jang","family":"Jin","sequence":"additional","affiliation":[{"name":"Department of Physiology, College of Medicine Kyung Hee University  Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8867-8822","authenticated-orcid":false,"given":"Sang\u2010Hoon","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Physiology, College of Medicine Kyung Hee University  Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4749-5795","authenticated-orcid":false,"given":"Chang\u2010Ju","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Physiology, College of Medicine Kyung Hee University  Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1832-1024","authenticated-orcid":false,"given":"Jae\u2010Joon","family":"Hwang","sequence":"additional","affiliation":[{"name":"Department of Pulmonary and Critical Care Medicine Gangdong Kyung Hee University Hospital  Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2536-6151","authenticated-orcid":false,"given":"Cheon Woong","family":"Choi","sequence":"additional","affiliation":[{"name":"Department of Pulmonary and Critical Care Medicine Gangdong Kyung Hee University Hospital  Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1623-2685","authenticated-orcid":false,"given":"Bok Soon","family":"Chang","sequence":"additional","affiliation":[{"name":"Department of Pulmonary and Critical Care Medicine Gangdong Kyung Hee University Hospital  Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,9,28]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.4046\/trd.2012.73.2.84"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fct.2007.01.014"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11356-018-3167-8"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00408-019-00200-z"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1155\/2011\/487074"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11869-010-0095-2"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajplung.00162.2006"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molimm.2018.05.003"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.toxlet.2011.01.031"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/tox.21750"},{"key":"e_1_2_8_12_1","first-page":"51","author":"Conti S.","year":"2018","journal-title":"Eur. 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