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Thus, in this study, we discovered novel pyrazole analogs as a potent nuclear factor kappa B (NF\u2010\u0138B) inhibitor. The compounds were assessed for NF\u2010\u0138B transcriptional inhibitory activity in RAW264.7 cells after stimulation with lipopolysaccharides (LPS), revealing Compound <jats:bold>6c<\/jats:bold> as the most potent analog among the tested series. The effect of Compound <jats:bold>6c<\/jats:bold> was further investigated on the levels of interleukin\u20101\u03b2, tumor necrosis factor\u2010\u03b1, and interleukin\u20106 in LPS\u2010stimulated RAW267.4 cells by enzyme immunoassay, where it causes a significant reduction in the level of these cytokines. In Western blot analysis, Compound <jats:bold>6c<\/jats:bold> also causes the inhibition of inhibitor kappa B\u2010\u03b1\u00a0and NF\u2010\u03baB. It was found to be snugly fitted into the inner grove of the active site of NF\u2010\u0138B by forming H\u2010bonds and a nonbonded interaction with Asn28 in a docking analysis.<\/jats:p>","DOI":"10.1002\/jbt.22656","type":"journal-article","created":{"date-parts":[[2020,10,23]],"date-time":"2020-10-23T13:30:49Z","timestamp":1603459849000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":48,"title":["Discovery of novel pyrazole derivatives as a potent anti\u2010inflammatory agent in RAW264.7 cells via inhibition of NF\u2010\u0138B for possible benefit against SARS\u2010CoV\u20102"],"prefix":"10.1002","volume":"35","author":[{"given":"Anup","family":"Masih","sequence":"first","affiliation":[{"name":"Drug Design &amp; Discovery Laboratory, Department of Pharmaceutical Sciences Sam Higginbottom University of Agriculture, Technology &amp; Sciences  Allahabad Uttar Pradesh India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amol K.","family":"Agnihotri","sequence":"additional","affiliation":[{"name":"Drug Design &amp; Discovery Laboratory, Department of Pharmaceutical Sciences Sam Higginbottom University of Agriculture, Technology &amp; Sciences  Allahabad Uttar Pradesh India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jitendra K.","family":"Srivastava","sequence":"additional","affiliation":[{"name":"Drug Design &amp; Discovery Laboratory, Department of Pharmaceutical Sciences Sam Higginbottom University of Agriculture, Technology &amp; Sciences  Allahabad Uttar Pradesh India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nidhi","family":"Pandey","sequence":"additional","affiliation":[{"name":"Department of Medicine and Health Sciences University Rovira i Virgili  Tarragona Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9643-4916","authenticated-orcid":false,"given":"Hans R.","family":"Bhat","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Sciences Dibrugarh University  Dibrugarh Assam India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0865-0502","authenticated-orcid":false,"given":"Udaya P.","family":"Singh","sequence":"additional","affiliation":[{"name":"Drug Design &amp; Discovery Laboratory, Department of Pharmaceutical Sciences Sam Higginbottom University of Agriculture, Technology &amp; Sciences  Allahabad Uttar Pradesh India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,10,23]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/tmi.13383"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(20)30627-9"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijsu.2020.02.034"},{"key":"e_1_2_7_5_1","first-page":"2006","volume":"24","author":"Kannan S.","year":"2020","journal-title":"Eur. 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