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The aim of this study is to investigate the potential toxic effect of aspartic acid\u2010coated magnesium oxide nanoparticles (Mg nano) and valproate (valp) using an in vitro three\u2010dimensional (3D) human liver organoid model and an in vivo pentylenetetrazole (PTZ)\u2010induced convulsion model in rats. Here, 3D human liver organoids were treated with valp or valp\u2009+\u2009Mg nano for 24 h\u00a0and then incubated with PTZ for an extra 24 h. As the in vivo model, rats were treated with valp, Mg nano, or valp\u2009+\u2009Mg nano for 4 weeks and\u00a0then they were treated with PTZ for 24 h. Toxicity in the liver organoids was demonstrated by reduced cell viability, decreased ATP, and increased reactive oxygen species. In the rat convulsion model, results revealed elevated serum alanine aminotransferase and aspartate aminotransferase levels. Both the in vitro and in vivo data demonstrated the potential toxic effects of valp\u2009+\u2009Mg nano on the liver tissues.<\/jats:p>","DOI":"10.1002\/jbt.22676","type":"journal-article","created":{"date-parts":[[2020,12,14]],"date-time":"2020-12-14T22:24:46Z","timestamp":1607984686000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["The potential toxic effects of magnesium oxide nanoparticles and valproate on liver tissue"],"prefix":"10.1002","volume":"35","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9857-4701","authenticated-orcid":false,"given":"Gehad","family":"Mekky","sequence":"first","affiliation":[{"name":"Zoology Department Faculty of Science  Zagazige Egypt"},{"name":"Wake Forest Institute for Regenerative Medicine  Winston\u2010Salem North Carolina USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Seeds","sequence":"additional","affiliation":[{"name":"Wake 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