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Eugenol and piperine have shown\u00a0many anticancer activities on numerous cancer types, like colon, lung, liver, and breast cancer. In this study, we amalgamated eugenol and piperine loaded with a polyhydroxy butyrate\/polyethylene glycol nanocomposite (Eu\u2010Pi\/PHB\u2010PEG\u2010NC) for better anticancer results against nasopharyngeal cancer (C666\u20101) cells. In the current study, nasopharyngeal cancer cell lines C666\u20101 were utilized to appraise the cytotoxic potential of Eug\u2010Pip\u2010PEG\u2010NC on cell propagation, programmed cell death, and relocation. Eu\u2010Pi\/PHB\u2010PEG\u2010NC inhibits cellular proliferation on C666\u20101 cells in a\u00a0dose\u2010dependent manner, and when compared with\u00a020\u2009\u00b5g\/ml, 15\u2009\u00b5g\/ml of loaded mixture evidently restrained the passage aptitude of C666\u20101 cells, this was attended with a downregulated expression of mitochondrial membrane potential. Treatment with 15\u2009\u00b5g\/ml Eu\u2010Pi\/PHB\u2010PEG\u2010NC suggestively amplified cell apoptosis in the C666\u20101 cells. Furthermore, its cleaved caspase\u20103, 8, and 9 and Bax gene expression was augmented and Bcl\u20102 gene expression was diminished after Eu\u2010Pi\/PHB\u2010PEG\u2010NC treatment. Additionally, our data established that the collective effect of Eu\u2010Pi\/PHB\u2010PEG\u2010NC loaded micelles inhibited the expansion of C666\u20101 cells augmented apoptosis connected with the intrusion of PI3K\/Akt\/mTOR signaling pathway.<\/jats:p>","DOI":"10.1002\/jbt.22700","type":"journal-article","created":{"date-parts":[[2021,1,15]],"date-time":"2021-01-15T00:14:17Z","timestamp":1610669657000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Eugenol\u2013piperine loaded polyhydroxy butyrate\/polyethylene glycol nanocomposite\u2010induced apoptosis and cell death in nasopharyngeal cancer (C666\u20101) cells through the inhibition of the PI3K\/AKT\/mTOR signaling pathway"],"prefix":"10.1002","volume":"35","author":[{"given":"Xiaopeng","family":"Sun","sequence":"first","affiliation":[{"name":"Department of Surgery, Faculty of Medicine Xi'an Jiaotong University  Xi'an China"},{"name":"Department of Otolaryngology Second Affiliated Hospital of Xi'an Medical College  Xi'an China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vishnu Priya","family":"Veeraraghavan","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Saveetha Dental College, Saveetha Institute of Medical and Technical Sciences Saveetha University  Chennai India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5204-5708","authenticated-orcid":false,"given":"Krishna Mohan","family":"Surapaneni","sequence":"additional","affiliation":[{"name":"Departments of Biochemistry, Clinical Skills &amp; 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