{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T15:03:32Z","timestamp":1787065412931,"version":"build-2736575974"},"reference-count":34,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2021,1,29]],"date-time":"2021-01-29T00:00:00Z","timestamp":1611878400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Biochem &amp; Molecular Tox"],"published-print":{"date-parts":[[2021,5]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>The field of nanotechnology offers great opportunities for cancer therapy.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>This study aimed to compare the therapeutic impact of Zn oxide nanoparticles (ZnO NPs) and thymoquinone (TQ) alone or as cotherapy in Ehrlich ascites carcinoma (EAC) induced in mice.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials and Methods<\/jats:title>\n                    <jats:p>This study was performed on 75 female albino mice divided into\u00a0Group I: EAC\u2010bearing control group, Group II: EAC treated with TQ, Group III: EAC treated with low\u2010dose ZnO NPs, Group IV: EAC treated with high\u2010dose ZnO NPs, Group V: EAC treated with TQ and low\u2010dose ZnO NPs. All groups were subjected to measurement of cell viability, ascites fluid volume, Bcl2 protein expression by Western blot analysis, cyclooxygenase 2 (COX2) gene expression by a real\u2010time polymerase chain reaction, enzyme\u2010linked immunosorbent assay levels of Beclin 1, interferon \u03b3 (INF\u03b3), interleukin 13 (IL\u201013), and estimation of Zn concentrations in EAC cells and liver homogenate to evaluate its toxicity.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Cotherapy has an efficient anticancer effect by enhancing apoptosis and autophagy, resulting in reducing tumor cell viability and ascites fluid volume together with downregulation of Bcl2 protein expression. This cotherapy increases Beclin 1 and INF\u03b3 and decreases IL\u201013. ZnO NPs upregulate COX2 expression, whereas TQ downregulates its expression. High\u2010dose ZnO NPs have more toxic effects on liver enzymes. Using TQ together with ZnO NPs can eliminate ZnO NPs liver toxicity.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>The cotherapy has an efficient anticancer effect by enhancing apoptosis and autophagy. High\u2010dose ZnO NPs have more toxic effects on liver enzymes. Using TQ together with ZnO NPs can eliminate ZnO NP liver toxicity.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/jbt.22736","type":"journal-article","created":{"date-parts":[[2021,1,29]],"date-time":"2021-01-29T09:25:50Z","timestamp":1611912350000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Impact of zinc oxide nanoparticles and thymoquinone in Ehrlich ascites carcinoma induced in mice"],"prefix":"10.1002","volume":"35","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7987-5111","authenticated-orcid":false,"given":"Heba B.","family":"Ghanem","sequence":"first","affiliation":[{"name":"Clinical Laboratory Sciences Department, College of Applied Medical Sciences Jouf University  Sakaka Saudi Arabia"},{"name":"Medical Biochemistry Department, Faculty of Medicine Tanta University  Tanta Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,1,29]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/1440-1681.13071"},{"issue":"2","key":"e_1_2_7_3_1","first-page":"1406","volume":"8","author":"Noureldeen A. 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