{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,15]],"date-time":"2026-06-15T04:12:53Z","timestamp":1781496773297,"version":"3.54.1"},"reference-count":55,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2021,5,28]],"date-time":"2021-05-28T00:00:00Z","timestamp":1622160000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["420386\/2018\u20101"],"award-info":[{"award-number":["420386\/2018\u20101"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["438384\/2018\u20100"],"award-info":[{"award-number":["438384\/2018\u20100"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Biochem &amp; Molecular Tox"],"published-print":{"date-parts":[[2021,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Some brain diseases are associated with oxidative stress and altered monoamine oxidase (MAO) activity. The objective of this study was to evaluate the antioxidant and neuroprotective actions through MAO inhibition of 3\u2010(pyridin\u20102\u2010yl)\u20102\u2010(pyridine\u20102\u2010ylimino) thiazolidin\u20104\u2010one (PPIT, a synthetic molecule containing a thiazolidinone nucleus), as well as its effects on toxicity parameters in Swiss female mice. Five in vitro assays were carried out to verify the PPIT antioxidant capacity: protein carbonylation (PC), 2,2'\u2010azino\u2010bis(3\u2010ethylbenzothiazoline\u20106\u2010sulfonic acid) (ABTS), 1,1\u2010diphenyl\u20102\u2010picryl\u2010hydrazil (DPPH), ferric ion (Fe<jats:sup>3+<\/jats:sup>) reducing antioxidant power (FRAP), and superoxide dismutase (SOD)\u2010like activity. The results showed that PPIT reduced the level of PC in the homogenate of the brain. This compound did not demonstrate SOD mimetic activity, but it acted as a free radical scavenger (ABTS and DPPH) and exhibited reducing activity in the FRAP assay. In addition, the effects of PPIT on cerebral MAO activity (MAO\u2010A and B isoforms) were investigated in vitro. Our data revealed inhibition of the MAO\u2010B activity by PPIT with no effects on MAO\u2010A. Lastly, an acute oral toxicity test was conducted in mice. No changes in food intake, body weight, and biochemical markers of kidney and liver damage were detected in mice treated with a high dose of PPIT (300\u2009mg\/kg). In conclusion, the present study demonstrated that PPIT exhibits antioxidant activity and selectively inhibits the MAO\u2010B isoform without causing apparent toxicity. These findings suggest PPIT as a potential therapeutic candidate to be tested in preclinical models of brain diseases involving perturbations of MAO\u2010B activity and redox status.<\/jats:p>","DOI":"10.1002\/jbt.22833","type":"journal-article","created":{"date-parts":[[2021,5,28]],"date-time":"2021-05-28T11:57:47Z","timestamp":1622203067000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Three \u2010(pyridin\u20102\u2010yl)\u20102\u2010(pyridin\u20102\u2010ylimino)thiazolidin\u20104\u2010one as a novel inhibitor of cerebral MAO\u2010B activity with antioxidant properties and low toxicity potential"],"prefix":"10.1002","volume":"35","author":[{"given":"Luiz Roberto","family":"Carraro Junior","sequence":"first","affiliation":[{"name":"Programa de P\u00f3s\u2010gradua\u00e7\u00e3o em Bioqu\u00edmica e Bioprospec\u00e7\u00e3o (PPGBBio), Laborat\u00f3rio de Bioqu\u00edmica e Neurofarmacologia Molecular (LABIONEM), Grupo de Pesquisa em Neurobiotecnologia (GPN), Centro de Ci\u00eancias Qu\u00edmicas, Farmac\u00eauticas e de Alimentos (CCQFA) Universidade Federal de Pelotas (UFPel)  Pelotas RS Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Am\u00e1lia Gon\u00e7alves","family":"Alves","sequence":"additional","affiliation":[{"name":"Programa de P\u00f3s\u2010gradua\u00e7\u00e3o em Bioqu\u00edmica e Bioprospec\u00e7\u00e3o (PPGBBio), Laborat\u00f3rio de Bioqu\u00edmica e Neurofarmacologia Molecular (LABIONEM), Grupo de Pesquisa em Neurobiotecnologia (GPN), Centro de Ci\u00eancias Qu\u00edmicas, Farmac\u00eauticas e de Alimentos (CCQFA) Universidade Federal de Pelotas (UFPel)  Pelotas RS Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ta\u00eds da Silva Teixeira","family":"Rech","sequence":"additional","affiliation":[{"name":"Programa de P\u00f3s\u2010gradua\u00e7\u00e3o em Bioqu\u00edmica e Bioprospec\u00e7\u00e3o (PPGBBio), Laborat\u00f3rio de Bioqu\u00edmica e Neurofarmacologia Molecular (LABIONEM), Grupo de Pesquisa em Neurobiotecnologia (GPN), Centro de Ci\u00eancias Qu\u00edmicas, Farmac\u00eauticas e de Alimentos (CCQFA) Universidade Federal de Pelotas (UFPel)  Pelotas RS Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3084-3020","authenticated-orcid":false,"given":"Jos\u00e9 Coan","family":"Campos J\u00fanior","sequence":"additional","affiliation":[{"name":"Programa de P\u00f3s\u2010Gradua\u00e7\u00e3o em Bioqu\u00edmica e Bioprospec\u00e7\u00e3o (PPGBBio), Laborat\u00f3rio de Qu\u00edmica Aplicada a Bioativos (LaQuiABio), Centro de Ci\u00eancias Qu\u00edmicas, Farmac\u00eauticas e de Alimentos Universidade Federal de Pelotas, UFPel  Pelotas RS Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Geonir Machado","family":"Siqueira","sequence":"additional","affiliation":[{"name":"Programa de P\u00f3s\u2010Gradua\u00e7\u00e3o em Bioqu\u00edmica e Bioprospec\u00e7\u00e3o (PPGBBio), Laborat\u00f3rio de Qu\u00edmica Aplicada a Bioativos (LaQuiABio), Centro de Ci\u00eancias Qu\u00edmicas, Farmac\u00eauticas e de Alimentos Universidade Federal de Pelotas, UFPel  Pelotas RS Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wilson","family":"Cunico","sequence":"additional","affiliation":[{"name":"Programa de P\u00f3s\u2010Gradua\u00e7\u00e3o em Bioqu\u00edmica e Bioprospec\u00e7\u00e3o (PPGBBio), Laborat\u00f3rio de Qu\u00edmica Aplicada a Bioativos (LaQuiABio), Centro de Ci\u00eancias Qu\u00edmicas, Farmac\u00eauticas e de Alimentos Universidade Federal de Pelotas, UFPel  Pelotas RS Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C\u00e9sar Augusto","family":"Br\u00fcning","sequence":"additional","affiliation":[{"name":"Programa de P\u00f3s\u2010gradua\u00e7\u00e3o em Bioqu\u00edmica e Bioprospec\u00e7\u00e3o (PPGBBio), Laborat\u00f3rio de Bioqu\u00edmica e Neurofarmacologia Molecular (LABIONEM), Grupo de Pesquisa em Neurobiotecnologia (GPN), Centro de Ci\u00eancias Qu\u00edmicas, Farmac\u00eauticas e de Alimentos (CCQFA) Universidade Federal de Pelotas (UFPel)  Pelotas RS Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9509-4446","authenticated-orcid":false,"given":"Cristiani Folharini","family":"Bortolatto","sequence":"additional","affiliation":[{"name":"Programa de P\u00f3s\u2010gradua\u00e7\u00e3o em Bioqu\u00edmica e Bioprospec\u00e7\u00e3o (PPGBBio), Laborat\u00f3rio de Bioqu\u00edmica e Neurofarmacologia Molecular (LABIONEM), Grupo de Pesquisa em Neurobiotecnologia (GPN), Centro de Ci\u00eancias Qu\u00edmicas, Farmac\u00eauticas e de Alimentos (CCQFA) Universidade Federal de Pelotas (UFPel)  Pelotas RS Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,5,28]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.18231\/j.ijn.2020.002"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules23081837"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms130810478"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.2174\/13816128113190990406"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/PL00005371"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2016.00340"},{"key":"e_1_2_9_8_1","first-page":"498","volume":"60","author":"Drozak J.","year":"2006","journal-title":"Postepy Hig Med. Dosw."},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1124\/jpet.117.241653"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a006148"},{"issue":"2","key":"e_1_2_9_11_1","volume":"1","author":"Wang S.","year":"2016","journal-title":"J. Alzheimers Parkinsonism Dement."},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.5607\/en.2011.20.1.1"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.2174\/1871527316666170124165222"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bioorg.2018.11.050"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.3109\/14756366.2014.1001755"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/ardp.200600118"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1039\/C5NJ00078E"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejmech.2016.04.012"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bioorg.2016.11.004"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11010-012-1328-5"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tetlet.2017.08.030"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bmc.2011.10.038"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tetasy.2014.01.012"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.3109\/14756366.2012.670805"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pnpbp.2020.109956"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1139\/cjpp-2019-0356"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/0076-6879(90)86141-H"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0891-5849(98)00315-3"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2008.08.008"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1006\/abio.1997.2522"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1974.tb03714.x"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0024-3205(01)01178-X"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-2952(65)90025-0"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(84)90780-2"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00427125"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12031-016-0743-6"},{"key":"e_1_2_9_37_1","doi-asserted-by":"crossref","unstructured":"OECD. (2006).OECD guidelines for the testing of chemicals Section 4.Test no. 423: acute oral toxicity \u2010 acute toxic class method. OECD Publishing Paris.https:\/\/doi.org\/10.1787\/9789264071001-en","DOI":"10.1787\/9789264071001-en"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1037\/0033-2909.83.3.482"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms141224438"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1124\/jpet.116.237503"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.2174\/1381612826666200131103524"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.2116\/analsci.18P014"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.1177\/1934578X1501001239"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jafc.9b02462"},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.1006\/abio.1996.0292"},{"key":"e_1_2_9_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bmc.2014.08.018"},{"key":"e_1_2_9_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejphar.2014.09.005"},{"key":"e_1_2_9_48_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1471-4159.1994.63030793.x"},{"key":"e_1_2_9_49_1","doi-asserted-by":"publisher","DOI":"10.1016\/bs.acc.2014.11.001"},{"key":"e_1_2_9_50_1","doi-asserted-by":"publisher","DOI":"10.5142\/jgr.2010.34.3.168"},{"key":"e_1_2_9_51_1","doi-asserted-by":"publisher","DOI":"10.1002\/mas.21375"},{"key":"e_1_2_9_52_1","doi-asserted-by":"publisher","DOI":"10.1002\/mas.21381"},{"key":"e_1_2_9_53_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10545-017-0104-9"},{"key":"e_1_2_9_54_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pharmthera.2014.02.010"},{"key":"e_1_2_9_55_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11030-013-9490-6"},{"key":"e_1_2_9_56_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-981-10-7757-9_5"}],"container-title":["Journal of Biochemical and Molecular Toxicology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jbt.22833","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jbt.22833","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jbt.22833","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,29]],"date-time":"2023-08-29T21:03:02Z","timestamp":1693342982000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jbt.22833"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,5,28]]},"references-count":55,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2021,8]]}},"alternative-id":["10.1002\/jbt.22833"],"URL":"https:\/\/doi.org\/10.1002\/jbt.22833","archive":["Portico"],"relation":{},"ISSN":["1095-6670","1099-0461"],"issn-type":[{"value":"1095-6670","type":"print"},{"value":"1099-0461","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,5,28]]},"assertion":[{"value":"2020-06-08","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-05-18","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-05-28","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e22833"}}