{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T10:44:01Z","timestamp":1790246641802,"version":"4.1.0"},"reference-count":45,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2021,8,4]],"date-time":"2021-08-04T00:00:00Z","timestamp":1628035200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Biochem &amp; Molecular Tox"],"published-print":{"date-parts":[[2021,10]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Alcoholic liver disease (ALD) is a\u00a0pervasive ailment due to the excessive consumption of alcohol and there is no operative drug for its treatment. The current exploration was intended to examine the hepatoprotective efficacy of arbutin against ethanol\u2010provoked liver injury in rats via the modulation of the Nrf\u20102\/HO\u20101 signaling cascade. Wistar rats were challenged with the 3\u2009g\/kg\/day (40% v\/v) of ethanol for 4 weeks to provoke the ALD and concomitantly supplemented with 40\u2009mg\/kg of arbutin. The liver function markers enzymes, inflammatory cytokines, and oxidative stress markers levels were scrutinized by using the respective assay kits. The mRNA expression of Nrf\u20102\/HO\u20101 signaling proteins was studied by reverse\u2010transcription polymerase chain reaction. The histological alterations of liver tissues were examined. HepG2 cells were used for the in vitro studies. The levels of oxidative stress markers and liver marker enzymes were examined by using kits. Reactive oxygen species (ROS)\u00a0and apoptotic cell death was detected by using fluorescent staining. There were no major differences in the body weight and liver weight of experimental animals. Arbutin treatment appreciably reduced the liver marker enzymes, upregulated superoxide dismutase, glutathione peroxidase, total antioxidant capacity, and the hydroxyl scavenging ability, and diminished the tumor necrosis factor\u2010\u03b1\u00a0and interleukin\u20106 levels in the serum of ethanol provoked animals. Arbutin triggered Nrf\u20102\/HO\u20101 signaling cascade liver tissues of ethanol\u2010provoked animals. Histological findings proved the preventing effects of arbutin. Arbutin did not demonstrate toxicity to the HepG2 cells. It reduced the aspartate aminotransferase and alanine aminotransferase, ROS, apoptotic cell death, lipid peroxidation and improved the antioxidants' levels in the ethanol\u2010challenged HepG2 cells. In conclusion, our findings unveiled the hepatoprotective efficacy of arbutin against ethanol\u2010provoked liver injury in rats. It could be a promising agent to treat alcoholic liver disease in the future.<\/jats:p>","DOI":"10.1002\/jbt.22872","type":"journal-article","created":{"date-parts":[[2021,8,4]],"date-time":"2021-08-04T03:47:21Z","timestamp":1628048841000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":42,"title":["Arbutin attenuates ethanol\u2010induced acute hepatic injury by the modulation of oxidative stress and Nrf\u20102\/HO\u20101 signaling pathway"],"prefix":"10.1002","volume":"35","author":[{"given":"Rongsheng","family":"Wang","sequence":"first","affiliation":[{"name":"Department of General Surgery The Second Affiliated Hospital of Wannan Medical College  Wuhu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7910-8537","authenticated-orcid":false,"given":"Jinji","family":"Mu","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology People's Hospital of Tongchuan  Tongchuan China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,8,4]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1155\/2018\/8284107"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.rcl.2008.02.008"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.22010"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.v20.i40.14672"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms161125942"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.v20.i47.17756"},{"key":"e_1_2_8_8_1","volume-title":"Global Status Report on Alcohol and Health (GSRAH)","year":"2018"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cgh.2013.06.013"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2016.02.042"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.v23.i36.6549"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.3390\/antiox9100980"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2018.04.162"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1165\/rcmb.2009-0170TR"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.abb.2010.03.019"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.toxlet.2017.04.009"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-0031-1276643"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12272-011-0420-9"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00011-012-0474-2"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.3109\/10715761003610760"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.2478\/intox-2014-0028"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.2165\/11536930-000000000-00000"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.23258"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.4254\/wjh.v12.i7.332"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1111\/add.14234"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1039\/C4FO00550C"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cgh.2018.11.033"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2017.09.030"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11130-013-0388-3"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fct.2013.11.043"},{"issue":"1459","key":"e_1_2_8_31_1","first-page":"1","volume":"10","author":"Liu X.","year":"2019","journal-title":"Frontiers\u00a0Pharmacol."},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M110.138636"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phymed.2011.12.006"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.alcohol.2010.07.004"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-018-36586-8"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1097\/WOX.0b013e3182439613"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.12691\/ajmbr-1-4-3"},{"issue":"5","key":"e_1_2_8_38_1","first-page":"285","volume":"59","author":"Pratt D. 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