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Previous studies have shown that acylpolyamines modulate glutamatergic receptors with the potential to alter pain pathways. This study aimed to evaluate the effects of mygalin on acute and chronic pain in rodents. For evaluation of acute pain, Wistar rats were subjected to tail\u2010flick and hot\u2010plate nociceptive tests. For the evaluation of chronic neuropathic pain, a partial ligation of the sciatic nerve was performed and, 21 days later, animals were examined in hot\u2010plate, tail\u2010flick, acetone, and von Frey tests. Either Mygalin or vehicle was microinjected in the dorsal raphe nucleus (DRN) before the tests. Another group was pretreated with selective antagonists of glutamate receptors (LY 235959, MK\u2010801, CNQX, and NBQX). Mygalin decreases nociceptive thresholds on both acute and chronic neuropathic pain models in all the tests performed. The lowest dose of mygalin yielded the most effective nociception, showing an increase of 63% of the nociceptive threshold of animals with neuropathic chronic pain. In\u00a0conclusion, mygalin microinjection in the DRN results in antinociceptive effect in models of neuropathic pain, suggesting that acylpolyamines and their derivatives, such as this diacylspermidine, could be pursued for the treatment of neuropathic pain and development of selective analgesics.<\/jats:p>","DOI":"10.1002\/jbt.22877","type":"journal-article","created":{"date-parts":[[2021,8,12]],"date-time":"2021-08-12T10:50:16Z","timestamp":1628765416000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["<i>Acanthoscurria gomesiana<\/i> spider\u2010derived synthetic mygalin in the dorsal raphe nucleus modulates acute and chronic pain"],"prefix":"10.1002","volume":"35","author":[{"given":"Ana C.","family":"Medeiros","sequence":"first","affiliation":[{"name":"Department of Biology Ribeir\u00e3o Preto School of Philosophy, Sciences and Literature of the University of S\u00e3o Paulo Ribeir\u00e3o Preto S\u00e3o Paulo Brazil"},{"name":"Behavioural Neurosciences Institute (INeC) Ribeir\u00e3o Preto S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Priscila","family":"Medeiros","sequence":"additional","affiliation":[{"name":"Behavioural Neurosciences Institute (INeC) Ribeir\u00e3o Preto S\u00e3o Paulo Brazil"},{"name":"Ribeir\u00e3o Preto Medical School of the University of S\u00e3o Paulo (FMRP\u2010USP) Ribeir\u00e3o Preto S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Renato L.","family":"de Freitas","sequence":"additional","affiliation":[{"name":"Behavioural Neurosciences Institute (INeC) Ribeir\u00e3o Preto S\u00e3o Paulo Brazil"},{"name":"Ribeir\u00e3o Preto Medical School of the University of S\u00e3o Paulo (FMRP\u2010USP) Ribeir\u00e3o Preto S\u00e3o Paulo Brazil"},{"name":"Department of Surgery and Anatomy Ribeir\u00e3o Preto Medical School of the University of S\u00e3o Paulo Ribeir\u00e3o Preto S\u00e3o Paulo Brazil"},{"name":"Biomedical Sciences Institute, Federal University of Alfenas (UNIFAL) Alfenas Minas Gerais Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pedro Ismael","family":"da Silva J\u00fanior","sequence":"additional","affiliation":[{"name":"Laboratory for Applied Toxinology, Center of Toxins, Immune\u2010Response and Cell Signaling (CeTICS\/CEPID) S\u00e3o Paulo S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Norberto C.","family":"Coimbra","sequence":"additional","affiliation":[{"name":"Behavioural Neurosciences Institute (INeC) Ribeir\u00e3o Preto S\u00e3o Paulo Brazil"},{"name":"Ribeir\u00e3o Preto Medical School of the University of S\u00e3o Paulo (FMRP\u2010USP) Ribeir\u00e3o Preto S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9371-279X","authenticated-orcid":false,"given":"Wagner F.","family":"dos Santos","sequence":"additional","affiliation":[{"name":"Department of Biology Ribeir\u00e3o Preto School of Philosophy, Sciences and Literature of the University of S\u00e3o Paulo Ribeir\u00e3o Preto S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2021,8,12]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1097\/j.pain.0000000000000641"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pain.2011.11.005"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1097\/j.pain.0000000000001340"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(99)01311-2"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1097\/j.pain.0000000000001365"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bcp.2011.04.015"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.4062\/biomolther.2012.097"},{"key":"e_1_2_9_9_1","doi-asserted-by":"crossref","unstructured":"R. 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