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This study aims to evaluate the promoter methylation status of the RECK gene and its serum level in patients with hepatitis C virus (HCV)\u2010related hepatocellular carcinoma (HCC) and the potential association of RECK gene methylation with clinical criteria of HCC. One hundred and fifty\u2010five subjects were included (healthy control [55], chronic HCV patients [55], HCV\u2010related HCC patients [45]). The methylation status of the RECK gene promoter and serum RECK level were investigated by methylation\u2010specific PCR and enzyme\u2010linked immunosorbent assay techniques, respectively. RECK gene promoter hypermethylation was recorded in 46.7% of HCC patients, and 10.9% of HCV patients, but not in control subjects (0%). It was related to RECK protein level, varices, edema, ascites, lymph node metastasis, vascular invasion, and the largest diameter of focal lesions. Meanwhile, it was not associated with focal lesion\u00a0number nor distant metastasis of HCC. In conclusion, RECK gene promoter hypermethylation is linked to HCV genotype\u20104\u2010related HCC. Moreover, different degrees of RECK gene promoter methylation are associated with serum RECK level, lymph node metastasis, and vascular invasion, which could prove its pathogenic role in\u00a0hepatocarcinogenesis in chronic HCV\u2010infected patients.<\/jats:p>","DOI":"10.1002\/jbt.22886","type":"journal-article","created":{"date-parts":[[2021,8,15]],"date-time":"2021-08-15T08:03:42Z","timestamp":1629014622000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Methylation degree of metalloproteinase inhibitor RECK gene: Links to RECK protein level and hepatocellular carcinoma in chronic HCV infection patients"],"prefix":"10.1002","volume":"35","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5449-2723","authenticated-orcid":false,"given":"Salwa M.","family":"Abo El\u2010khair","sequence":"first","affiliation":[{"name":"Medical Biochemistry and Molecular Biology Department, Faculty of Medicine Mansoura University  Mansoura Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hatem","family":"Elalfy","sequence":"additional","affiliation":[{"name":"Tropical Medicine Department, Mansoura Faculty of Medicine Mansoura University  Mansoura Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Muhammad","family":"Diasty","sequence":"additional","affiliation":[{"name":"Tropical Medicine Department, Mansoura Faculty of Medicine Mansoura University  Mansoura Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eman E.","family":"Ebrahim","sequence":"additional","affiliation":[{"name":"Tropical Medicine Department, Mansoura Faculty of Medicine Mansoura University  Mansoura Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ayman Z.","family":"Elsamanoudy","sequence":"additional","affiliation":[{"name":"Medical Biochemistry and Molecular Biology Department, Faculty of Medicine Mansoura University  Mansoura Egypt"},{"name":"Clinical Biochemistry Department, Faculty of Medicine King Abdulaziz University  Jeddah Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2021,8,15]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.oraloncology.2008.02.004"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.7150\/ijbs.4038"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.3892\/ol.2013.1306"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1245\/s10434-010-1431-3"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.soc.2014.09.001"},{"key":"e_1_2_10_7_1","first-page":"718","volume":"28","author":"Holan N. 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