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Autophagy is critical for inhibiting production of NLRP3 protein that effectively reduces the inflammatory response. Ginsenoside Rg3 (SY), an active component extracted from ginseng, is reported to protect against CP\u2010induced nephrotoxicity. However, the mechanisms underlying renoprotection by SY have not been established to date. Our results indicate that SY attenuated CP\u2010induced apoptosis and damage in vivo and in vitro, as evidenced by increased cell viability, decreased the proportion of late apoptotic cells, elevated mitochondrial membrane potential, and ameliorated histopathological damage of the kidney. SY ameliorated CP\u2010induced human renal tubular (HK\u20102) cells and kidney injury through upregulation of LC3II\/I and beclin\u20101, inhibition of p62, NLRP3, ASC, caspase\u20101, and interleukin\u20101\u03b2. However, blockade of autophagy by 3\u2010methyladenine reversed the suppression of SY on NLRP3 inflammasome activation and the protection of SY on HK\u20102 cells. Our collective results support the utility of SY as a therapeutic agent that effectively protects against CP\u2010induced kidney injury by activating the autophagy\u2010mediated NLRP3 inhibition pathway.<\/jats:p>","DOI":"10.1002\/jbt.22896","type":"journal-article","created":{"date-parts":[[2021,8,23]],"date-time":"2021-08-23T08:25:51Z","timestamp":1629707151000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["Ginsenoside Rg3 attenuates cisplatin\u2010induced kidney injury through inhibition of apoptosis and autophagy\u2010inhibited NLRP3"],"prefix":"10.1002","volume":"35","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7707-7928","authenticated-orcid":false,"given":"Jinghui","family":"Zhai","sequence":"first","affiliation":[{"name":"Department of Pharmacy The First Hospital of Jilin University, Changchun  Jilin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5072-841X","authenticated-orcid":false,"given":"Huan","family":"Gao","sequence":"additional","affiliation":[{"name":"Department of Pharmacy The First Hospital of Jilin University, Changchun  Jilin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuo","family":"Wang","sequence":"additional","affiliation":[{"name":"Research and Development Department Dalian Fusheng Natural Medicine Development Co., Ltd.  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