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Visnagin (VIS) is a natural bioactive component of\n                    <jats:italic>Ammi visnaga<\/jats:italic>\n                    , with promising radical scavenging and anti\u2010inflammatory activities. This study explored the protective effect of VIS against isoproterenol (ISO)\u2010induced acute myocardial injury and oxidative stress in rats. VIS was supplemented for 14 days, and the rats received ISO (100\u2009mg\/kg) twice at an interval of 24\u2009h. ISO\u2010induced myocardial injury was characterized by elevated serum CK\u2010MB, LDH, and troponin\u2010I associated with increased heart weight\u00a0and several histopathological changes. ISO increased reactive oxygen species (ROS), malondialdehyde (MDA), NF\u2010\n                    <jats:italic>\u03ba<\/jats:italic>\n                    B p65, TNF\u2010\u03b1, IL\u20106, and decreased glutathione and antioxidant enzymes in rats' hearts. VIS prevented myocardial injury and ameliorated the cardiac function markers, ROS, MDA, NF\u2010\n                    <jats:italic>\u03ba<\/jats:italic>\n                    B p65, and pro\u2010inflammatory cytokines in ISO\u2010intoxicated rats. In addition, VIS decreased Bax mRNA and caspases, and upregulated Nrf2, HO\u20101, Bcl\u20102, and PPAR\u03b3. Molecular docking simulations revealed the binding method of VIS to NF\u2010\n                    <jats:italic>\u03ba<\/jats:italic>\n                    B, Keap1, and PPAR\u03b3. In conclusion, VIS protects against ISO\u2010induced acute myocardial injury by attenuating oxidative tissue injury and reducing key inflammatory and apoptosis markers. In vivo and\n                    <jats:italic>in silico<\/jats:italic>\n                    results showed that activation of Nrf2\/HO\u20101 signaling and PPAR\u03b3 mediates the cardioprotective effect of VIS.\n                  <\/jats:p>","DOI":"10.1002\/jbt.22906","type":"journal-article","created":{"date-parts":[[2021,9,6]],"date-time":"2021-09-06T02:28:36Z","timestamp":1630895316000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":43,"title":["Visnagin prevents isoproterenol\u2010induced myocardial injury by attenuating oxidative stress and inflammation and upregulating Nrf2 signaling in rats"],"prefix":"10.1002","volume":"35","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4018-6925","authenticated-orcid":false,"given":"Mohammad H.","family":"Abukhalil","sequence":"first","affiliation":[{"name":"Department of Biology, Faculty of Science Al\u2010Hussein Bin Talal University Ma'an Jordan"},{"name":"Department of Medical Analysis, Princess Aisha Bint Al\u2010Hussein Faculty of Nursing and Health Sciences Al\u2010Hussein Bin Talal University Ma'an Jordan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Omnia E.","family":"Hussein","sequence":"additional","affiliation":[{"name":"Physiology Division, Zoology Department, Faculty of Science Beni\u2010Suef University Beni\u2010Suef Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Saleem H.","family":"Aladaileh","sequence":"additional","affiliation":[{"name":"Department of Medical Analysis, Princess Aisha Bint Al\u2010Hussein Faculty of Nursing and Health Sciences Al\u2010Hussein Bin Talal University Ma'an Jordan"},{"name":"Department of Pharmacy Practice, College of Pharmacy University of Hafr Al\u2010Batin Hafr Al\u2010Batin Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3406-9634","authenticated-orcid":false,"given":"Osama Y.","family":"Althunibat","sequence":"additional","affiliation":[{"name":"Department of Medical Analysis, Princess Aisha Bint Al\u2010Hussein Faculty of Nursing and Health Sciences Al\u2010Hussein Bin Talal University Ma'an Jordan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wesam","family":"Al\u2010Amarat","sequence":"additional","affiliation":[{"name":"Department of Medical Support, Al\u2010karak University College Al\u2010Balqa' Applied University As\u2010Salt Jordan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sultan A.","family":"Saghir","sequence":"additional","affiliation":[{"name":"Department of Medical Analysis, Princess Aisha Bint Al\u2010Hussein Faculty of Nursing and Health Sciences Al\u2010Hussein Bin Talal University Ma'an Jordan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manal A.","family":"Alfwuaires","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, Faculty of Science King Faisal University Al\u2010Ahsa Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abdulmohsen I.","family":"Algefare","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, Faculty of Science King Faisal University Al\u2010Ahsa Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Khalid M.","family":"Alanazi","sequence":"additional","affiliation":[{"name":"Zoology Department, College of Science King Saud University Riyadh Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Farhan K.","family":"Al\u2010Swailmi","sequence":"additional","affiliation":[{"name":"Department of Pharmacy Practice, College of Pharmacy University of Hafr Al\u2010Batin Hafr Al\u2010Batin Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emadeldin M.","family":"Kamel","sequence":"additional","affiliation":[{"name":"Chemistry Department, Faculty of Science Beni\u2010Suef University Beni\u2010Suef Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0279-6500","authenticated-orcid":false,"given":"Ayman M.","family":"Mahmoud","sequence":"additional","affiliation":[{"name":"Physiology Division, Zoology Department, Faculty of Science Beni\u2010Suef University Beni\u2010Suef Egypt"},{"name":"Biotechnology Department, Research Institute of Medicinal and Aromatic Plants Beni\u2010Suef University Beni\u2010Suef Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,9,5]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S2214-109X(19)30318-3"},{"issue":"4","key":"e_1_2_9_3_1","first-page":"1841","volume":"5","author":"Frangogiannis N. 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