{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T09:55:46Z","timestamp":1753869346702,"version":"3.41.2"},"reference-count":24,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2021,11,10]],"date-time":"2021-11-10T00:00:00Z","timestamp":1636502400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100012226","name":"Fundamental Research Funds for the Central Universities","doi-asserted-by":"publisher","award":["xzy012019078"],"award-info":[{"award-number":["xzy012019078"]}],"id":[{"id":"10.13039\/501100012226","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100007128","name":"Natural Science Foundation of Shaanxi Province","doi-asserted-by":"publisher","award":["2018JQ8019"],"award-info":[{"award-number":["2018JQ8019"]}],"id":[{"id":"10.13039\/501100007128","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Biochem &amp; Molecular Tox"],"published-print":{"date-parts":[[2022,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The outbreak of coronavirus disease 2019 (COVID\u201019) has induced a large number of deaths worldwide. Angiotensin\u2010converting enzyme 2 (ACE2) is the entry receptor for the 2019 novel coronavirus (2019\u2010nCoV) to infect the host cells. Therefore, ACE2 may be an important target for the prevention and treatment of COVID\u201019. The aim of this study was to investigate the inhibition effect of valaciclovir hydrochloride (VACV), zidovudine (ZDV), saquinavir (SQV), and efavirenz (EFV) on 2019\u2010nCoV infection. The results of molecule docking and surface plasmon resonance showed that VACV, ZDV, SQV, and EFV could bind to ACE2 protein, with the <jats:italic>K<\/jats:italic><jats:sub>D<\/jats:sub> value of (4.33\u2009\u00b1\u20090.09) e<jats:sup>\u22128<\/jats:sup>, (6.29\u2009\u00b1\u20091.12) e<jats:sup>\u22126<\/jats:sup>, (2.37\u2009\u00b1\u20090.59) e<jats:sup>\u22125<\/jats:sup>, and (4.85\u2009\u00b1\u20091.57) e<jats:sup>\u22125<\/jats:sup> M, respectively. But only ZDV and EFV prevent the 2019\u2010nCoV spike pseudotyped virus to enter ACE2\u2010HEK293T cells with an EC<jats:sub>50<\/jats:sub> value of 4.30\u2009\u00b1\u20091.46 and 3.92\u2009\u00b1\u20091.36\u2009\u03bcM, respectively. ZDV and EFV also have a synergistic effect on preventing entry of virus into cells. In conclusion, ZDV and EFV suppress 2019\u2010nCoV infection of ACE2\u2010HEK293T cells by interacting with ACE2.<\/jats:p>","DOI":"10.1002\/jbt.22948","type":"journal-article","created":{"date-parts":[[2021,11,10]],"date-time":"2021-11-10T11:42:49Z","timestamp":1636544569000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Antiviral drugs suppress infection of 2019\u2010nCoV spike pseudotyped virus by interacting with ACE2 protein"],"prefix":"10.1002","volume":"36","author":[{"given":"Jue","family":"Wang","sequence":"first","affiliation":[{"name":"School of Pharmacy, Health Science Center Xi'an Jiaotong University Xi'an Shaanxi China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yongjing","family":"Zhang","sequence":"additional","affiliation":[{"name":"School of Pharmacy, Health Science Center Xi'an Jiaotong University Xi'an Shaanxi 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