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This study\u00a0involves 200 participants (100 healthy controls, 50 steroid\u2010sensitive nephrotic syndromes [SSNS] patients, and 50 steroid\u2010resistant nephrotic syndromes [SRNS] patients]. Genomic DNA has been characterized using the PCR\u2010RFLP technique. The predominant genotype that is common in this study population was the<jats:italic>EDN1 3A\/3A<\/jats:italic>genotype (NS [75%] and healthy controls [88%]). The prevalence of<jats:italic>EDN1 3A\/4A<\/jats:italic>genotype and<jats:italic>EDN1 4A<\/jats:italic>allele was significantly increased among NS patients compared with healthy subjects (<jats:italic>p<\/jats:italic>\u2010value\u2009&lt;\u20090.05). Furthermore, the frequency of the<jats:italic>EDN1<\/jats:italic>(3A\/4A; rs1800997) variant was statistically significant among SRNS patients (<jats:italic>p<\/jats:italic>\u2010value\u2009&lt;\u20090.05). The<jats:italic>EDN1<\/jats:italic>3A\/4A genotype and the<jats:italic>EDN1<\/jats:italic>4A allele were identified as independent risk factors of the nephrotic syndrome among children.<\/jats:p>","DOI":"10.1002\/jbt.22963","type":"journal-article","created":{"date-parts":[[2021,11,16]],"date-time":"2021-11-16T09:24:24Z","timestamp":1637054664000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Genetic variant in the 5\u02b9 untranslated region of endothelin1 (EDN1) gene in children with primary nephrotic syndrome"],"prefix":"10.1002","volume":"36","author":[{"given":"Sohier","family":"Yahia","sequence":"first","affiliation":[{"name":"Department of Pediatrics, Faculty of Medicine Mansoura University Mansoura Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ayman","family":"Hammad","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Faculty of Medicine Mansoura University Mansoura Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Abdel\u2010Hady","family":"El\u2010Gilany","sequence":"additional","affiliation":[{"name":"Department of Public Health, Faculty of Medicine Mansoura University Mansoura Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mohamed","family":"El\u2010Assmy","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Faculty of Medicine Mansoura University Mansoura Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rasha","family":"El\u2010Tanbouly","sequence":"additional","affiliation":[{"name":"Resident of Pediatrics and Neonatology Aga Central Hospital Aga Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Afaf M.","family":"Elsaid","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Genetics Unit, Children Hospital Mansoura University Mansoura Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lamiaa Z.","family":"Elmoursi","sequence":"additional","affiliation":[{"name":"Department of Clinical Pathology, Student Hospital Mansoura University Mansoura Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3381-2641","authenticated-orcid":false,"given":"Rami M.","family":"Elshazli","sequence":"additional","affiliation":[{"name":"Department of Basic Sciences, Biochemistry and Molecular Genetics Unit, Faculty of Physical Therapy Horus University\u2009\u2010\u2009Egypt New Damietta Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rasha MS","family":"Shoaib","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Faculty of Science Damietta University Damietta Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2021,11,16]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1080\/20469047.2017.1374003"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(03)14184-0"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.3310\/hta23260"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(14)60654-1"},{"issue":"6","key":"e_1_2_11_6_1","first-page":"479","volume":"93","author":"Kodner C.","year":"2016","journal-title":"Am. 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