{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T04:08:19Z","timestamp":1789445299046,"version":"build-2803163510"},"reference-count":88,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2022,2,18]],"date-time":"2022-02-18T00:00:00Z","timestamp":1645142400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Biochem &amp; Molecular Tox"],"published-print":{"date-parts":[[2022,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:label\/><jats:p>The current study investigates the anti\u2010inflammatory and hepatoprotective effects of selenium (Se) formulated as nanoparticles (SeNPs) and in combination with quercetin (QCT) against thioacetamide (TAA)\u2010induced hepatocellular carcinoma (HCC) in rats. \u200b\u200b\u200b\u200b\u200b\u200bSeventy\u2010two male Sprague\u2013Dawley rats were divided into six groups (<jats:italic>n<\/jats:italic>\u2009=\u200912). Three control groups; normal, SeNPs; group received SeNPs only and HCC; group received TAA. In addition, three preventive groups; SeNPs\u2009+\u2009TAA, QCT\u2009+\u2009TAA, and QCT\u2009+\u2009SeNPs\u2009+\u2009TAA. Induction of HCC was detected histopathologically and by the raise of the serum level of alpha\u2010fetoprotein (AFP). Oxidative stress was evaluated by the hepatic levels of reduced glutathione (GSH), glutathione peroxidase (GPx), and malondialdehyde (MDA) spectrophotometrically. The oncogenic pathway of p53\/\u03b2\u2010catenin\/cyclin D1 was assessed by immunohistochemistry. The inflammatory markers; interleukin\u201033 (IL\u201033), IL\u20106, and IL\u20101\u03b2\u00a0were assessed by enzyme\u2010linked immune sorbent assay. SeNPs prevented the elevation of serum AFP and hepatic IL\u201033, IL\u20101\u03b2, and IL\u20106 in comparison to HCC or QCT\u2009+\u2009TAA groups. SeNPs\u2009+\u2009TAA exhibited a lower positive hepatic staining of p53, \u03b2\u2010catenin, and cyclin D1 in comparison to HCC or QCT\u2009+\u2009TAA groups. Moreover, SeNPs improved the overall oxidative balance indicated by low hepatic MDA and enhanced GSH and GPx when compared to HCC or QCT\u2009+\u2009TAA groups. \u200b\u200bSeNPs alone and in combination with QCT were found to suppress the progression of HCC in rats via the enhancement of the oxidative stress and then inflammatory status and the prevention of the deregulation of the oncogenic axis pathway of p53\/\u03b2\u2010catenin\/cyclin D.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jbt.22989","type":"journal-article","created":{"date-parts":[[2022,2,18]],"date-time":"2022-02-18T11:14:24Z","timestamp":1645182864000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":36,"title":["Selenium nanoparticles and quercetin suppress thioacetamide\u2010induced hepatocellular carcinoma in rats: Attenuation of inflammation involvement"],"prefix":"10.1002","volume":"36","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2810-7016","authenticated-orcid":false,"given":"Ahmed A.","family":"Mohamed","sequence":"first","affiliation":[{"name":"Department of Biochemistry, Faculty of Pharmacy Sinai University  Cairo Egypt"},{"name":"Department of Biochemistry, Faculty of Pharmacy Mansoura University  Mansoura Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Randa A.","family":"Zaghloul","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Faculty of Pharmacy Mansoura University  Mansoura Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amr M.","family":"Abdelghany","sequence":"additional","affiliation":[{"name":"Department of Spectroscopy, Physics Division National Research Center  Giza Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amal M.","family":"El Gayar","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Faculty of Pharmacy Mansoura University  Mansoura Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,2,18]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12032-021-01510-0"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.3892\/or.2013.2917"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2018.08.104"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1089\/ars.2009.2973"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s40520-018-1086-7"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.3892\/ol.2011.378"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1166\/jnn.2011.4130"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11051-012-1236-3"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jinorgbio.2007.06.021"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms21051585"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/mnfr.200700393"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamcr.2017.06.017"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0157251"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1158\/1940-6207.CAPR-13-0383"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/cam4.2388"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/mnfr.201300362"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2014.00141"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cyto.2007.09.013"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.25670"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10620-011-1760-5"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1177\/030006051204000504"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2018.02506"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00203-020-01831-0"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10876-020-01856-x"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1080\/24701556.2017.1357611"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1093\/toxsci\/kfm221"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10585-015-9721-6"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1080\/13651820410024049"},{"key":"e_1_2_8_30_1","first-page":"9","volume":"2014","author":"Chan S. T.","year":"2014","journal-title":"BioMed Res. Int."},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0676.1990.tb00428.x"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/0168-8278(95)80226-6"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4939-7802-1_11"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1093\/ajcp\/28.1.56"},{"key":"e_1_2_8_35_1","first-page":"48","volume":"4","author":"Gregor A.","year":"1977","journal-title":"Infusions Ther. Klin Ernahrung \u2010 Forsch\u00a0Prax"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1093\/clinchem\/27.4.636a"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(79)90738-3"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpba.2008.04.028"},{"key":"e_1_2_8_39_1","first-page":"158","volume":"70","author":"Paglia D. E.","year":"1967","journal-title":"J. Lab. Clin. Med."},{"key":"e_1_2_8_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(11)61347-0"},{"key":"e_1_2_8_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbagen.2013.01.007"},{"key":"e_1_2_8_42_1","doi-asserted-by":"publisher","DOI":"10.3945\/jn.112.172395"},{"key":"e_1_2_8_43_1","doi-asserted-by":"publisher","DOI":"10.1080\/01635581.2016.1145245"},{"key":"e_1_2_8_44_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0172838"},{"key":"e_1_2_8_45_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0109424"},{"key":"e_1_2_8_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ghir.2005.06.015"},{"key":"e_1_2_8_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tox.2007.09.007"},{"key":"e_1_2_8_48_1","doi-asserted-by":"publisher","DOI":"10.1093\/alcalc\/agh074"},{"key":"e_1_2_8_49_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.108.2814.626"},{"key":"e_1_2_8_50_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cca.2016.10.006"},{"key":"e_1_2_8_51_1","doi-asserted-by":"publisher","DOI":"10.1586\/14737140.2.6.709"},{"key":"e_1_2_8_52_1","doi-asserted-by":"publisher","DOI":"10.2174\/1566524033479582"},{"key":"e_1_2_8_53_1","first-page":"5","volume":"24","author":"Shaban N. Z.","year":"2017","journal-title":"J. Biol. Res."},{"key":"e_1_2_8_54_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-020-03536-5"},{"key":"e_1_2_8_55_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10620-009-0990-2"},{"key":"e_1_2_8_56_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbi.2018.08.016"},{"key":"e_1_2_8_57_1","doi-asserted-by":"publisher","DOI":"10.3390\/nu12030857"},{"key":"e_1_2_8_58_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbagen.2014.10.008"},{"issue":"10","key":"e_1_2_8_59_1","first-page":"1","volume":"31","author":"Ahmed O. M.","year":"2019","journal-title":"Drug Chem. Toxicol."},{"key":"e_1_2_8_60_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-016-0028-x"},{"key":"e_1_2_8_61_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12011-019-01793-5"},{"key":"e_1_2_8_62_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-017-16105-x"},{"key":"e_1_2_8_63_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.rpor.2020.04.004"},{"key":"e_1_2_8_64_1","doi-asserted-by":"publisher","DOI":"10.3332\/ecancer.2017.721"},{"key":"e_1_2_8_65_1","doi-asserted-by":"publisher","DOI":"10.4254\/wjh.v10.i1.1"},{"key":"e_1_2_8_66_1","doi-asserted-by":"publisher","DOI":"10.1038\/cmi.2016.62"},{"key":"e_1_2_8_67_1","doi-asserted-by":"publisher","DOI":"10.1002\/mc.22491"},{"key":"e_1_2_8_68_1","doi-asserted-by":"publisher","DOI":"10.17980\/2016.380"},{"key":"e_1_2_8_69_1","first-page":"191","volume":"7","author":"Shalby A. B.","year":"2017","journal-title":"J. Appl. Pharm. Sci."},{"key":"e_1_2_8_70_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2020.110851"},{"key":"e_1_2_8_71_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2012.05.012"},{"key":"e_1_2_8_72_1","first-page":"450","volume":"8","author":"Inagawa S.","year":"2002","journal-title":"Clin. Cancer Res."},{"key":"e_1_2_8_73_1","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-11-2322"},{"key":"e_1_2_8_74_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2017.07.026"},{"key":"e_1_2_8_75_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2018.12.146"},{"key":"e_1_2_8_76_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-405882-8.00004-0"},{"key":"e_1_2_8_77_1","doi-asserted-by":"publisher","DOI":"10.1002\/mc.22318"},{"key":"e_1_2_8_78_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.25853"},{"key":"e_1_2_8_79_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-0676.2001.021002089.x"},{"key":"e_1_2_8_80_1","doi-asserted-by":"publisher","DOI":"10.1177\/1010428317698361"},{"key":"e_1_2_8_81_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12957-020-01850-7"},{"key":"e_1_2_8_82_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2019.116933"},{"key":"e_1_2_8_83_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.92.19.8876"},{"key":"e_1_2_8_84_1","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.v13.i7.985"},{"key":"e_1_2_8_85_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1008361818480"},{"key":"e_1_2_8_86_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.20689"},{"key":"e_1_2_8_87_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.274.5293.1672"},{"key":"e_1_2_8_88_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41420-020-0287-y"},{"key":"e_1_2_8_89_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms13089649"}],"container-title":["Journal of Biochemical and Molecular Toxicology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jbt.22989","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jbt.22989","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jbt.22989","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,25]],"date-time":"2023-08-25T20:04:06Z","timestamp":1692993846000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jbt.22989"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,2,18]]},"references-count":88,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2022,4]]}},"alternative-id":["10.1002\/jbt.22989"],"URL":"https:\/\/doi.org\/10.1002\/jbt.22989","archive":["Portico"],"relation":{},"ISSN":["1095-6670","1099-0461"],"issn-type":[{"value":"1095-6670","type":"print"},{"value":"1099-0461","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,2,18]]},"assertion":[{"value":"2021-05-31","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-01-05","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-02-18","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e22989"}}