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Allopurinol for XO and acetazolamide for carbonic anhydrase isoenzymes were used as positive standards in inhibition studies. In addition, compounds <jats:bold>8<\/jats:bold> and <jats:bold>9<\/jats:bold>, which were obtained in the intermediate step, were also investigated for their inhibition effects against the three enzymes. According to the enzyme inhibition results, hybrid isoindole\u20101,3(2<jats:italic>H<\/jats:italic>)\u2010dione derivatives <jats:bold>10<\/jats:bold> and <jats:bold>11<\/jats:bold> showed significant inhibitory effects against all three enzymes. Surprisingly, compound <jats:bold>8<\/jats:bold>, containing a SCN functional group, exhibited a greater inhibitory effect than the other compounds against hCA I and hCA II. The IC<jats:sub>50<\/jats:sub> values of compound <jats:bold>8<\/jats:bold> against hCA I and hCA II were found to be 3.698\u2009\u00b1\u20090.079 and 3.147\u2009\u00b1\u20090.083\u2009\u00b5M, respectively. Compound <jats:bold>8<\/jats:bold> (IC<jats:sub>50<\/jats:sub>\u2009=\u20094.261\u2009\u00b1\u20090.034\u2009\u03bcM) showed higher activity than allopurinol (IC<jats:sub>50<\/jats:sub>\u2009=\u20094.678\u2009\u00b1\u20090.029\u2009\u03bcM) and the other compounds against XO, as well. These results clearly show the effect of the SCN group on the inhibition. In addition, in silico molecular docking studies were performed to understand the molecular interactions between each compound and enzymes, and the results were evaluated.<\/jats:p>","DOI":"10.1002\/jbt.23015","type":"journal-article","created":{"date-parts":[[2022,3,8]],"date-time":"2022-03-08T05:59:03Z","timestamp":1646719143000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Novel hybrid isoindole\u20101,3(2<i>H<\/i>)\u2010dione compounds containing a 1<i>H<\/i>\u2010tetrazole moiety: Synthesis, biological evaluation, and molecular docking studies"],"prefix":"10.1002","volume":"36","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2692-7923","authenticated-orcid":false,"given":"Ayse","family":"Tan","sequence":"first","affiliation":[{"name":"Department of Food Processing, Vocational School of Technical Sciences Mus Alparslan University Mus Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1398-3881","authenticated-orcid":false,"given":"Serap","family":"Kizilkaya","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Faculty of Arts and Sciences Mus Alparslan University Mus Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4165-0045","authenticated-orcid":false,"given":"Samir A. 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