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However, little is known about the intrarenal regulation of the release and expression of this calcium\u2010regulating protein during kidney injury. We sought to characterize the time\u2010dependent expression and excretion of the protein calbindin in the distal tubule in comparison to kidney injury molecule\u20101 (Kim\u20101), a protein in the proximal tubule, in mice treated with cisplatin. Urine, blood, and kidneys were collected from male C57BL\/6 mice treated with vehicle or cisplatin (20\u2009mg\/kg\u00a0ip). Urinary concentrations of calbindin and Kim\u20101 were elevated by 11.6\u2010fold and 2.5\u2010fold, respectively, within 2 days after cisplatin. Circulating creatinine and blood urea nitrogen levels increased in cisplatin\u2010treated mice by 3 days, confirming the development of acute kidney injury. Time\u2010dependent decreases in intrarenal calbindin protein were observed on Days 3 and 4 and a 200\u2010fold upregulation of calbindin (<jats:italic>CALB1)<\/jats:italic> and <jats:italic>KIM\u20101<\/jats:italic> messenger RNAs\u00a0(mRNAs) was observed on Day 3. These data suggest that early loss of calbindin protein into the urine along with declines in renal calbindin levels initiates a compensatory induction of mRNA expression at later time points (Days 3 and 4). Understanding the regulation of calbindin during cisplatin nephrotoxicity further enhances its utility as a potential urinary biomarker of kidney damage. The results of the current study support the combined use of a proximal (Kim\u20101) and distal tubule (calbindin) marker to phenotype acute kidney injury secondary to cisplatin administration.<\/jats:p>","DOI":"10.1002\/jbt.23068","type":"journal-article","created":{"date-parts":[[2022,4,11]],"date-time":"2022-04-11T06:32:08Z","timestamp":1649658728000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Regulation of renal calbindin expression during cisplatin\u2010induced kidney injury"],"prefix":"10.1002","volume":"36","author":[{"given":"Blessy","family":"George","sequence":"first","affiliation":[{"name":"Department of Pharmacology and Toxicology, Ernest Mario School of Pharmacy Rutgers University Piscataway New Jersey USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John T.","family":"Szilagyi","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Toxicology, Ernest 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