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Thus, the present study aims to clarify the mechanisms of CBZ\u2010induced hepatorenal toxicity in rats. Therefore, we partitioned 40 male Wistar rats into four groups (<jats:italic>n<\/jats:italic>\u2009=\u200910): a negative control group and three treatment groups, which received 100, 300, and 600\u2009mg\/kg of CBZ. All rats received the treatment daily by oral gavage. We collected blood and organ samples (liver and kidney) at 14 and 28 days postdosing. CBZ caused extensive pathological alterations in both the liver and kidneys, such as cellular degeneration and necrosis accompanied by severe inflammatory reactions in a dose\u2010 and time\u2010dependent manner. All the CBZ\u2010treated groups displayed strong tumor necrosis factor\u2010\u03b1 and nuclear factor\u2010\u03baB\u00a0(NF\u2010\u03baB) immunopositivity. Additionally, CBZ dose\u2010dependently elevated the alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, urea, and creatinine serum levels and reduced the serum albumin levels. Furthermore, CBZ\u2010induced apoptosis, as indicated by the observed Bax gene upregulation and Bcl\u20102 gene downregulation in both organs. All these changes may be related to oxidative stress, as indicated by the increase in malondialdehyde levels and the decrease in total antioxidant capacity. Our results demonstrate that CBZ\u2010induced dose\u2010 and time\u2010dependent hepatorenal damage through oxidative stress, which activated both the NF\u2010\u03baB signaling pathway and Bcl\u2010based programmed cell death.<\/jats:p>","DOI":"10.1002\/jbt.23079","type":"journal-article","created":{"date-parts":[[2022,4,19]],"date-time":"2022-04-19T08:08:33Z","timestamp":1650355713000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Novel insights into the potential mechanisms underlying carbendazim\u2010induced hepatorenal toxicity in rats"],"prefix":"10.1002","volume":"36","author":[{"given":"Yasmin A.","family":"Ebedy","sequence":"first","affiliation":[{"name":"Pathology Department, Faculty of Veterinary Medicine Cairo University Giza Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohamed O.","family":"Elshazly","sequence":"additional","affiliation":[{"name":"Pathology Department, Faculty of Veterinary Medicine Cairo University Giza Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Neven H.","family":"Hassan","sequence":"additional","affiliation":[{"name":"Physiology Department, Faculty of Veterinary Medicine Cairo University Giza Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7935-8379","authenticated-orcid":false,"given":"Marwa A.","family":"Ibrahim","sequence":"additional","affiliation":[{"name":"Biochemistry Department, Faculty of Veterinary Medicine Cairo University Giza Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3670-6841","authenticated-orcid":false,"given":"Eman I.","family":"Hassanen","sequence":"additional","affiliation":[{"name":"Pathology Department, Faculty of Veterinary Medicine Cairo University Giza Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,4,19]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chemosphere.2015.11.018"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1094\/PHYTO-99-4-0441"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.geoderma.2010.10.003"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.4314\/ijbcsv8i5.3"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2613.2012.00828.x"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.talanta.2014.10.056"},{"key":"e_1_2_9_8_1","first-page":"505","volume":"63","author":"Lutz P.","year":"2012","journal-title":"Medycyna Pracy"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1742-7843.2011.00831.x"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.3109\/15376516.2014.928764"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.3109\/15376516.2015.1020182"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.etap.2013.01.010"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1093\/mutage\/geu084"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fsi.2014.09.037"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/bdrb.20290"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.3892\/mmr.2015.3381"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1382-6689(02)00126-6"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tox.2012.01.010"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/tox.20660"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.3109\/19390211.2015.1107802"},{"key":"e_1_2_9_21_1","first-page":"303","volume":"9","author":"Abdel\u2010Mobdy Y. 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