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Hepatic stellate cells (HSCs) are the main cells involved in the formation of hepatic fibrosis, and induction of the apoptosis of HSCs is an important strategy for the treatment of hepatic fibrosis. Diallyl trisulfide (DATS) is a natural product and is the main active ingredient in garlic. However, the exact molecular mechanisms underlying HSC apoptosis induced by DATS are not well understood. This study aimed to analyze the efficiency and mechanism of DATS in hepatic fibrosis. Different concentrations (25, 50, 100, and 200\u2009\u03bcM) of DATS were used to treat HSCs. Changes in cell morphology and formation of apoptotic bodies were observed under an inverted microscope and an electric microscope. Bcl\u20102 signaling involving Bax, Caspase\u20103, Caspase\u20106, Caspase\u20108, Caspase\u20109, p53, Apaf\u20101, and Cyto\u2010c in fibrosis were examined, which is a critical step in the evaluation of antihepatic fibrosis agents. We also evaluated the effect of DATS on the cellular morphology of HSCs and apoptosis\u2010related factors under different Bcl\u20102 expression states. Our results suggest that DATS regulates hepatic fibrosis by blocking the Bcl\u20102 signaling pathway and upregulating the Bax\/Bcl\u20102 ratio.<\/jats:p>","DOI":"10.1002\/jbt.23097","type":"journal-article","created":{"date-parts":[[2022,5,9]],"date-time":"2022-05-09T12:44:32Z","timestamp":1652100272000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Diallyl trisulfide plays an antifibrotic role by inhibiting the expression of Bcl\u20102 in hepatic stellate cells"],"prefix":"10.1002","volume":"36","author":[{"given":"Huai","family":"Pang","sequence":"first","affiliation":[{"name":"Laboratory of Xinjiang Endemic and Ethnic Diseases Shihezi University School of Medicine Shihezi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cuizhe","family":"Wang","sequence":"additional","affiliation":[{"name":"Laboratory of Xinjiang Endemic and Ethnic Diseases Shihezi University School of Medicine Shihezi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Ye","sequence":"additional","affiliation":[{"name":"Department of Psychology People's Hospital of Xinjiang Uygur Autonomous Region Urumqi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lulu","family":"Wang","sequence":"additional","affiliation":[{"name":"Center of Community Health Services, The First Affiliated Hospital, Medical College Shihezi University Shihezi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoming","family":"Zhou","sequence":"additional","affiliation":[{"name":"Department of Pathology Hainan University School of Medicine Haikou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaomeng","family":"Ge","sequence":"additional","affiliation":[{"name":"CAS Key Laboratory of Genome Science and Information Beijing Institute of Genomics, Chinese Academy of Sciences Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun","family":"Zhang","sequence":"additional","affiliation":[{"name":"Laboratory of Xinjiang Endemic and Ethnic Diseases Shihezi University School of Medicine Shihezi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qinghua","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Oncology People's Hospital of Deyang City Deyang Sichuan Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,5,9]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2019.01.045"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41419-018-1272-8"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1159\/000343852"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.clinre.2015.06.015"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.addr.2017.05.007"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrgastro.2017.38"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrgastro.2017.38"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbt.22470"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0899-3467(07)60149-9"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1093\/jn\/131.3.951S"},{"key":"e_1_2_8_12_1","doi-asserted-by":"crossref","first-page":"1780956","DOI":"10.1155\/2018\/1780956","volume":"2018","author":"Alam R. 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