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Mitoquinone (MitoQ), a newly designed mitochondria\u2010targeted antioxidant, has been proved for its benefit in mitochondria protection. However, the role of MitoQ in PQ\u2010induced lung injury remains unclear. Thus, this study was performed to investigate the effect of MitoQ on PQ\u2010induced lung injury and its underlying mechanisms. Our work showed that PQ caused the inhibition of A549 lung epithelial cell viability in a dose\u2010dependent manner, while MitoQ remarkably mitigated the PQ\u2010induced cell viability suppression. Besides this, PQ\u2010mediated apoptosis of A549 cells was significantly attenuated by MitoQ, as indicated by the TUNEL assay and mitochondria membrane potential assay. Moreover, the intracellular reactive oxygen species (ROS) production was also dramatically suppressed when cotreated MitoQ with PQ. This could be ascribed to enhanced mitochondrial fusion mediated by Mitofusin 1 (MFN1)\/Mitofusin 2 (MFN2), because MitoQ preserved mitochondrial network integrity, as reflected by MitoTracker staining, and MitoQ also increased the expression of MFN1\/MFN2 in A549 cells after PQ treatment. Our data suggested MitoQ mitigated PQ\u2010induced lung epithelial cell injury by promoting MFN1\/MFN2\u2010mediated mitochondrial fusion, and MitoQ might be a potential candidate drug for the treatment of PQ\u2010induced lung injury.<\/jats:p>","DOI":"10.1002\/jbt.23127","type":"journal-article","created":{"date-parts":[[2022,6,10]],"date-time":"2022-06-10T06:58:56Z","timestamp":1654844336000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Mitoquinone mitigates paraquat\u2010induced A549 lung epithelial cell injury by promoting MFN1\/MFN2\u2010mediated mitochondrial fusion"],"prefix":"10.1002","volume":"36","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0315-2195","authenticated-orcid":false,"given":"Chao","family":"Liu","sequence":"first","affiliation":[{"name":"Department of Emergency Medicine, Jinling Hospital Medical School of Nanjing University  Nanjing PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhaorui","family":"Sun","sequence":"additional","affiliation":[{"name":"Department of Emergency Medicine, Jinling Hospital Medical School of Nanjing University  Nanjing PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mengmeng","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Emergency Medicine, Jinling Hospital Medical School of Nanjing University  Nanjing PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhizhou","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Emergency Medicine, Jinling Hospital Medical School of Nanjing University  Nanjing PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Emergency Medicine, Jinling Hospital Medical School of Nanjing University  Nanjing PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi","family":"Ren","sequence":"additional","affiliation":[{"name":"Department of Emergency Medicine, Jinling Hospital Medical School of Nanjing University  Nanjing PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoqin","family":"Han","sequence":"additional","affiliation":[{"name":"Department of Emergency Medicine, Jinling Hospital Medical School of Nanjing University  Nanjing PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bo","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Emergency Medicine, Jinling Hospital Medical School of Nanjing University  Nanjing PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mengya","family":"Yao","sequence":"additional","affiliation":[{"name":"Department of Emergency Medicine, Jinling Hospital Medical School of Nanjing University  Nanjing PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shinan","family":"Nie","sequence":"additional","affiliation":[{"name":"Department of Emergency Medicine, Jinling Hospital Medical School of Nanjing University  Nanjing PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,6,9]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1097\/CCM.0000000000002950"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(13)62137-6"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10157-019-01702-z"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1745-7270.2008.00386.x"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules24081498"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbt.22739"},{"issue":"11","key":"e_1_2_8_8_1","first-page":"1043","volume":"29","author":"Sun B.","year":"2017","journal-title":"Zhonghua wei zhong bing ji jiu yi xue"},{"key":"e_1_2_8_9_1","first-page":"1854206","volume":"2020","author":"Zhang Z.","year":"2020","journal-title":"Oxid. 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