{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,13]],"date-time":"2026-01-13T07:42:28Z","timestamp":1768290148078,"version":"3.49.0"},"reference-count":30,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2022,6,21]],"date-time":"2022-06-21T00:00:00Z","timestamp":1655769600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81970786"],"award-info":[{"award-number":["81970786"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Biochem &amp; Molecular Tox"],"published-print":{"date-parts":[[2022,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Posterior capsular opacification (PCO) is the major complication after cataract surgery and can result in secondary vision loss. Circular RNAs (circRNAs) are reported to play critical regulatory roles in multiple cell biological processes. The most common working mechanism of circRNAs is by acting as microRNA\u00a0sponges. Here, we analyzed the role and mechanism of circRNA RNA polymerase III subunit A (POLR3A) in PCO. Cell viability was analyzed by 3\u2010(4,5\u2010dimethylthiazol\u20102\u2010yl)\u20102,5\u2010diphenyltetrazolium bromide\u00a0assay. Cell motility was assessed by transwell and wound healing assays. Dual\u2010luciferase reporter and RNA\u2010pull\u2010down\u00a0assays were performed to verify the interaction between microRNA\u201031 (miR\u201031) and circ\u2010POLR3A or thioredoxin interacting protein (TXNIP). PCO cell model was established by treating SRA01\/04 cells with transforming growth factor\u2010\u03b22 (TGF\u2010\u03b22). We found that TGF\u2010\u03b22 enhanced SRA01\/04 cell viability, migration, and invasion abilities. Circ\u2010POLR3A expression was upregulated in PCO tissues and TGF\u2010\u03b22\u2010induced SRA01\/04 cells. TGF\u2010\u03b22 promoted the viability and motility of SRA01\/04 cells largely by upregulating circ\u2010POLR3A. Circ\u2010POLR3A negatively regulated the miR\u201031 level by directly interacting with it. Circ\u2010POLR3A absence\u2010induced influences in TGF\u2010\u03b22\u2010induced SRA01\/04 cells were partly reversed by silencing miR\u201031. miR\u201031 is directly bound to the 3\u2032\u2010untranslated region\u00a0of TXNIP. TXNIP overexpression largely attenuated miR\u201031 overexpression\u2010mediated effects in TGF\u2010\u03b22\u2010induced SRA01\/04 cells. Circ\u2010POLR3A could elevate the protein expression of TXNIP by sponging miR\u201031. Exosomes were involved in mediating the delivery of circ\u2010POLR3A in SRA01\/04 cells. In conclusion, circ\u2010POLR3A contributed to TGF\u2010\u03b22\u2010induced promotion of cell viability, migration, and invasion of SRA01\/04 cells by targeting miR\u201031\/TXNIP axis.<\/jats:p>","DOI":"10.1002\/jbt.23144","type":"journal-article","created":{"date-parts":[[2022,6,22]],"date-time":"2022-06-22T06:34:25Z","timestamp":1655879665000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Circ\u2010POLR3A accelerates TGF\u2010\u03b22\u2010induced promotion in cell viability, migration, and invasion of lens epithelial cells via miR\u201031\/TXNIP signaling cascade"],"prefix":"10.1002","volume":"36","author":[{"given":"Huajun","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Ophthalmology The First Affiliated Hospital of Zhengzhou University Zhengzhou City Henan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3763-1477","authenticated-orcid":false,"given":"Guangying","family":"Zheng","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology The First Affiliated Hospital of Zhengzhou University Zhengzhou City Henan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Miaomiao","family":"Sun","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology The First Affiliated Hospital of Zhengzhou University Zhengzhou City Henan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yingjie","family":"Chi","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology The First Affiliated Hospital of Zhengzhou University Zhengzhou City Henan China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2022,6,21]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1097\/00055735-200002000-00009"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0039-6257(92)90073-3"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1001\/archophthalmol.2009.3"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12943-017-0630-y"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.117.029004"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.17-22698"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.omtn.2017.02.008"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2018.06.149"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1534\/g3.113.008698"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.2119\/molmed.2011.00463"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12038-017-9709-2"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.12-10904"},{"key":"e_1_2_10_14_1","first-page":"4","volume":"5","author":"Singh L. 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