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The mouse myocardial tissues were subject to hematoxylin\u2013eosin (HE) staining, TUNEL assay, and immunofluorescence analysis. H9c2 cell transfection and hypoxia induction were then completed, and cell apoptosis and cytokine levels were tested. Additionally, RNA pull\u2010down and dual luciferase reporter gene assays were conducted for exploring the relation of miR\u2010208 with programmed cell death 4 (PDCD4). Additionally, fluorescence in situ hybridization (FISH) was conducted for investigating miR\u2010208 and PDCD4 colocalization within H9c2 cells. AMI mice had severe damage, apoptosis, decreased miR\u2010208 expression, increased IL\u20101\u03b2, IL\u20106, IL\u20108 levels, whereas reduced IL\u201010 level within myocardial tissues. H9c2 cells under hypoxia induction exhibited decreased miR\u2010208 expression, promoted apoptosis, increased protein expression of Bax and cleaved\u2010caspase\u20103, decreased protein expression of Bcl\u20102 and caspase\u20103, elevated IL\u20101\u03b2, IL\u20106, IL\u20108 levels and decreased IL\u201010 level. miR\u2010208 upregulation alleviated the damage and apoptosis of myocardial tissues in AMI mice. AMI mice with miR\u2010208 upregulation showed decreased expression of Bax and cleaved\u2010caspase\u20103, increased expression of Bcl\u20102 and caspase\u20103, reduced levels of IL\u20101\u03b2, IL\u20106, IL\u20108, whereas an increased level of IL\u201010. miR\u2010208 showed direct inhibition of PDCD4. PDCD4 and miR\u2010208 were mainly co\u2010expressed in the cytoplasm. The upregulated PDCD4 expression abolished miR\u2010208's suppression of H9c2 cell apoptosis induced by hypoxia. Besides this, miR\u2010208 inhibited myocardial tissue apoptosis in AMI mice by inhibiting PDCD4 expression.<\/jats:p>","DOI":"10.1002\/jbt.23202","type":"journal-article","created":{"date-parts":[[2022,9,10]],"date-time":"2022-09-10T06:39:32Z","timestamp":1662791972000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["miR\u2010208 inhibits myocardial tissues apoptosis in mice with acute myocardial infarction by targeting inhibition of PDCD4"],"prefix":"10.1002","volume":"36","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2719-8809","authenticated-orcid":false,"given":"Qiang","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Cardio\u2010thoracic Surgery, Wujin Hospital Affiliated with Jiangsu University The Wujin Clinical College of Xuzhou Medical University  Changzhou People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huxiang","family":"Zhou","sequence":"additional","affiliation":[{"name":"Medical College of Jiangsu University  Zhenjiang People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaobo","family":"Zhu","sequence":"additional","affiliation":[{"name":"Department of Cardio\u2010thoracic Surgery, Wujin Hospital Affiliated with Jiangsu University The Wujin Clinical College of Xuzhou Medical University  Changzhou People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Feng","family":"Jiang","sequence":"additional","affiliation":[{"name":"Department of Cardio\u2010thoracic Surgery, Wujin Hospital Affiliated with Jiangsu University The Wujin Clinical College of Xuzhou Medical University  Changzhou People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiuhua","family":"Yu","sequence":"additional","affiliation":[{"name":"Department of Cardio\u2010thoracic Surgery, Wujin Hospital Affiliated with Jiangsu University The Wujin Clinical College of Xuzhou Medical University  Changzhou People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junjie","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Cardio\u2010thoracic Surgery, Wujin Hospital Affiliated with Jiangsu University The Wujin Clinical College of Xuzhou Medical University  Changzhou People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yaxiang","family":"Ji","sequence":"additional","affiliation":[{"name":"Department of Cardiac Ultrasound, Wujin Hospital Affiliated with Jiangsu University The Wujin Clinical College of Xuzhou Medical University  Changzhou People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,9,9]]},"reference":[{"issue":"10","key":"e_1_2_8_2_1","first-page":"87","volume":"21","author":"Xiaojia L.","year":"2019","journal-title":"J. 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