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Both autophagy and apoptosis are involved in the regulation of dental fluorosis as well as in protein synthesis and enamel mineralization. Emerging evidence suggests that autophagy and apoptosis are interconnected and that their interaction greatly influences cell death. However, the effect of autophagy on apoptosis in fluoride\u2010treated ameloblasts is unclear. Here, we employed an in vitro cellular model of fluorosis in mouse ameloblast\u2010like LS8 cells and induced autophagy using sodium fluoride (NaF). Our findings suggest that NaF treatment induces autophagy in LS8 cells, and ATG5 and ATG7 are important molecules involved in this process. We also showed that NaF treatment reduced cell viability in Atg5\/7 siRNA and autophagy inhibitor\u2010treated LS8 cells. More importantly, NaF\u2010induced apoptosis can be reversed by inhibiting early stage of autophagy. In conclusion, our study shows that autophagy is closely related to dental fluorosis, and inhibition of autophagy, especially ATG5\/7, reduces fluoride\u2010induced cell death and apoptosis.<\/jats:p>","DOI":"10.1002\/jbt.23280","type":"journal-article","created":{"date-parts":[[2022,12,20]],"date-time":"2022-12-20T04:44:44Z","timestamp":1671511484000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Inhibition of autophagy reduces the rate of fluoride\u2010induced LS8 apoptosis via regulating ATG5 and ATG7"],"prefix":"10.1002","volume":"37","author":[{"given":"Lin","family":"Zhao","sequence":"first","affiliation":[{"name":"Ningxia Key Laboratory of Cranio\u2010maxillofacial Deformities, College of Stomatology Ningxia Medical University  Yinchuan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Han","family":"Wang","sequence":"additional","affiliation":[{"name":"Ningxia Key Laboratory of 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