{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T00:03:41Z","timestamp":1790899421879,"version":"4.1.0"},"reference-count":23,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2023,2,2]],"date-time":"2023-02-02T00:00:00Z","timestamp":1675296000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Biochem &amp; Molecular Tox"],"published-print":{"date-parts":[[2023,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Methicillin\u2010resistant <jats:italic>Staphylococcus aureus<\/jats:italic> (MRSA) is a highly infectious Gram\u2010positive pathogen known to cause severe diseases such as endocarditis, food poisoning, pneumonia, osteomyelitis, and septicemia. MRSA is a major public health issue. Among these, osteomyelitis is inflammation of the bone caused by the invasion of the bacterial pathogen in the bones. Its prominent symptoms include fever, pain, and redness of bones. In the case of children, it affects the long bones of arms and legs, whereas in the case of adults it affects the hip, feet, and spine. Bacterial osteomyelitis can trigger pathological remodeling of bones and hence causes substantial morbidity and mortality. The present study aims to evaluate the isoflavone genistein's (5,7\u2010dihydroxy\u20103\u2010(4\u2010hydroxyphenyl)\u22124H\u20101\u2010benzopyran\u20104\u2010one,4\u2032,5,7 trihydroxyisoflavone) antimicrobial and anti\u2010inflammatory effects against osteomyelitis induced by MRSA in male Wistar rats. Classification of the animals was into the following: sham (Group I), osteomyelitis (Group II, control), genistein (25\u2009mg\/kg body weight, Group III), and genistein (50\u2009mg\/kg body weight, Group IV). The rats did not receive any treatment for 4 weeks after bacterial inoculation. Genistein was then administered twice daily for 2 weeks. Bacterial growth, mean body weight bone infection status, and side effects of genistein treatment were assessed. Furthermore, lipid peroxidation, superoxide dismutase, glutathione (GSH) peroxidase, catalase, reduced GSH, tumor necrosis factor\u2010\u03b1 (TNF\u2010\u03b1), and interleukin (IL)\u20106 were also determined. Two days after treatment, it was found that genistein significantly suppressed bacterial growth and reduced serum pro\u2010inflammatory cytokines TNF\u2010\u03b1 and IL\u20106. Therefore, the study suggests that genistein could be a promising lead against MRSA\u2010induced osteomyelitis.<\/jats:p>","DOI":"10.1002\/jbt.23298","type":"journal-article","created":{"date-parts":[[2023,2,2]],"date-time":"2023-02-02T09:26:58Z","timestamp":1675330018000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Antibacterial and anti\u2010inflammatory effects of genistein in <i>Staphylococcus aureus<\/i> induced osteomyelitis in rats"],"prefix":"10.1002","volume":"37","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5102-2275","authenticated-orcid":false,"given":"Xinyi","family":"Guo","sequence":"first","affiliation":[{"name":"Department of Orthopaedic Surgery, Shanxi Bethune Hospital, Shanxi Academy of Medical Sciences, Tongji Shanxi Hospital Third Hosptial of Shanxi Medical University  Taiyuan China"},{"name":"Department of Orthopaedic Surgery, Tongji Hospital, Tongji Medical College Huazhong University of Science and Technology  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,2,2]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.2147\/IDR.S272733"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00932-19"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-020-18268-0"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2021.638085"},{"key":"e_1_2_9_6_1","first-page":"1","volume":"2021","author":"Sharifi\u2010Rad J.","year":"2021","journal-title":"Oxid. 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