{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T22:20:07Z","timestamp":1785450007580,"version":"3.56.0"},"reference-count":26,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2023,1,16]],"date-time":"2023-01-16T00:00:00Z","timestamp":1673827200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Biochem &amp;amp; Molecular Tox"],"published-print":{"date-parts":[[2023,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>This study aimed to explore the role of dual specificity phosphatase 12 (DUSP12) in regulating myocardial ischemia\u2013reperfusion (I\/R) injury and the underlying mechanism. The expression of DUSP12 in myocardial tissues\u00a0and heat\u2010shock protein beta\u20108\u00a0(HSPB8) and mitophagy\u2010related proteins in myocardial tissues and H9c2 cells were detected by western blot analysis. The serum creatine kinase isoenzymes (CK\u2010MB)\u00a0and lactate dehydrogenase (LDH), levels of reactive oxygen species\u00a0and malondialdehyde,\u00a0superoxide dismutase activity in myocardial tissues\u00a0and H9c2 cells, and caspase\u20103 activity in H9c2 cells were analyzed by corresponding assay kits. The infarct area in the\u00a0rat's heart was observed by triphenyl tetrazolium chloride\u00a0staining. The apoptosis of myocardial cells in myocardial tissues and H9c2 cells was detected by terminal\u2010deoxynucleotidyl transferase dUTP\u2010biotin nick\u2010end labeling\u00a0assay. The interaction between DUSP12 and HSPB8 was clarified by the coimmunoprecipitation assay. The transfection efficacy of si\u2010HSPB8#1 and si\u2010HSPB8#2 in H9c2 cells was confirmed by real\u2010time quantitative\u2010polymerase\u00a0chain reaction\u00a0and western blot analysis. As a result, DUSP12 expression was downregulated in I\/R rats, which was promoted by lentivirus\u2010expressing DUSP12. DUSP12 overexpression reduced the serum\u00a0creatine kinase isoenzymes (CK\u2010MB) and LDH, decreased the infarct area in the rat's heart, and suppressed the apoptosis and oxidative stress in myocardial tissues. DUSP12 overexpression also upregulated the expression of HSPB8 to promote mitophagy. The coimmunoprecipitation assay indicated that DUSP12 could be combined with HSPB8. In addition, DUSP12 overexpression could inhibit hypoxia\/reoxygenation\u2010elicited apoptosis as well as oxidative stress in H9c2 cells by upregulating HSPB8 expression to promote mitophagy, which was countervailed by HSPB8 deficiency. In conclusion, DUSP12 overexpression decreased the apoptosis and oxidative stress in myocardial I\/R injury through HSPB8\u2010induced mitophagy.<\/jats:p>","DOI":"10.1002\/jbt.23310","type":"journal-article","created":{"date-parts":[[2023,1,16]],"date-time":"2023-01-16T10:00:58Z","timestamp":1673863258000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["DUSP12 ameliorates myocardial ischemia\u2013reperfusion injury through HSPB8\u2010induced mitophagy"],"prefix":"10.1002","volume":"37","author":[{"given":"Jing","family":"Cheng","sequence":"first","affiliation":[{"name":"Department of Anesthesiology Henan Provincial People's Hospital  Zhengzhou Henan China"},{"name":"Department of Anesthesiology of Central China Fuwai Hospital Central China Fuwai Hospital of Zhengzhou University  Zhengzhou Henan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meihua","family":"Ji","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology Henan Provincial People's Hospital  Zhengzhou Henan China"},{"name":"Department of Anesthesiology of Central China Fuwai Hospital Central China Fuwai Hospital of Zhengzhou University  Zhengzhou Henan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haijuan","family":"Jing","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology Henan Provincial People's Hospital  Zhengzhou Henan China"},{"name":"Department of Anesthesiology of Central China Fuwai Hospital Central China Fuwai Hospital of Zhengzhou University  Zhengzhou Henan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6711-4200","authenticated-orcid":false,"given":"Hongqi","family":"Lin","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology Henan Provincial People's Hospital  Zhengzhou Henan China"},{"name":"Department of Anesthesiology of Central China Fuwai Hospital Central China Fuwai Hospital of Zhengzhou University  Zhengzhou Henan China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,1,16]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbadis.2020.165768"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/adfm.201803567"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra071667"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1042\/BJ20082234"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.7150\/ijbs.57130"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1042\/CS20160664"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00395-016-0536-7"},{"key":"e_1_2_9_9_1","doi-asserted-by":"crossref","DOI":"10.1161\/JAHA.119.014311","volume":"10","author":"Zhao J.","year":"2021","journal-title":"J. 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