{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,7]],"date-time":"2026-07-07T16:13:01Z","timestamp":1783440781537,"version":"3.54.6"},"reference-count":56,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2023,4,4]],"date-time":"2023-04-04T00:00:00Z","timestamp":1680566400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100010728","name":"ICAR-Indian Veterinary Research Institute","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100010728","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Biochem &amp; Molecular Tox"],"published-print":{"date-parts":[[2023,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Biochanin\u2010A (BCA),\u00a0is an isoflavonoid, exhibits protective effects against various diseases. This study was conducted to observe the effect of BCA on isoprenaline (ISP)\u2010induced\u00a0cardiac fibrosis and explore the underlying mechanism. The curative effect of BCA was investigated with oral administration for 14 days in ISP\u2010induced\u00a0cardiac fibrosis in mice. The fibrotic biomarkers, like collagen I and III, were estimated by ELISA. Commercial kits were used to estimate cholesterol, triglycerides, and creatine kinase\u2010myocardial band (CK\u2010MB)\u00a0levels. The\u00a0messenger ribonucleic acid\u00a0(mRNA) expression studies were performed by quantitative real\u2010time polymerase chain reaction. Gelatin zymography was used to study the expression of matrix metalloproteinases\u20102 (<jats:italic>MMP\u20102<\/jats:italic>).\u00a0BCA\u00a0co\u2010administration significantly improved the morphometric parameters; including heart weight, heart weight to body weight, heart weight to tibial length, and lipid profile. BCA treatment showed a reduction in inflammatory cells and collagen deposition as depicted in the histopathology of heart tissues. The enhanced levels of collagen\u2010I, III, and hydroxyproline were significantly decreased by BCA co\u2010treatment, whereas CK\u2010MB\u00a0level was reduced slightly. BCA co\u2010administration\u00a0increased the activity of reduced glutathione enzyme, showing the antioxidative\u00a0effects of BCA. BCA\u00a0treatment significantly reduced interleukin\u20106 (<jats:italic>Il6<\/jats:italic>) inflammatory cytokine along with partially decreased mRNA expression of fibrotic signaling markers such as natriuretic peptide type B (<jats:italic>Nppb<\/jats:italic>), \u03b1\u2010smooth muscle actin (<jats:italic>Acta2<\/jats:italic>), connective tissue growth factor (<jats:italic>Ctgf<\/jats:italic>), transforming growth factor \u03b2 (<jats:italic>Tgfb)<\/jats:italic>, small mothers against decapentaplegic homolog\u20103 (<jats:italic>Smad\u20103<\/jats:italic>). However, BCA did not modify <jats:italic>Mmp\u20102<\/jats:italic>\u00a0expression, which was significantly increased by ISP. In conclusion, BCA exerts an antifibrotic effect by modulating lipid profile, enhancing antioxidant enzyme, and reducing collagen content and inflammation.<\/jats:p>","DOI":"10.1002\/jbt.23360","type":"journal-article","created":{"date-parts":[[2023,4,5]],"date-time":"2023-04-05T03:28:51Z","timestamp":1680665331000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Biochanin\u2010A alleviates fibrosis and inflammation in cardiac injury in mice"],"prefix":"10.1002","volume":"37","author":[{"given":"Meemansha","family":"Sharma","sequence":"first","affiliation":[{"name":"Division of Pharmacology and Toxicology ICAR\u2010Indian Veterinary Research Institute Bareilly Uttar Pradesh India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6800-8437","authenticated-orcid":false,"given":"Thakur Uttam","family":"Singh","sequence":"additional","affiliation":[{"name":"Division of Pharmacology and Toxicology ICAR\u2010Indian Veterinary Research Institute Bareilly Uttar Pradesh India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abhinav","family":"Rana","sequence":"additional","affiliation":[{"name":"Division of Pharmacology and Toxicology ICAR\u2010Indian Veterinary Research Institute Bareilly Uttar Pradesh India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tarun","family":"Kumar","sequence":"additional","affiliation":[{"name":"Division of Pharmacology and Toxicology ICAR\u2010Indian Veterinary Research Institute Bareilly Uttar Pradesh India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manju","family":"Gari","sequence":"additional","affiliation":[{"name":"Division of Pharmacology and Toxicology ICAR\u2010Indian Veterinary Research Institute Bareilly Uttar Pradesh India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pashupathi","family":"Mani","sequence":"additional","affiliation":[{"name":"Division of Animal Biochemistry ICAR\u2010Indian Veterinary Research Institute Bareilly Uttar Pradesh India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3266-3701","authenticated-orcid":false,"given":"Madhu Cholenahalli","family":"Lingaraju","sequence":"additional","affiliation":[{"name":"Division of Pharmacology and Toxicology ICAR\u2010Indian Veterinary Research Institute Bareilly Uttar Pradesh India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8406-5260","authenticated-orcid":false,"given":"Subhashree","family":"Parida","sequence":"additional","affiliation":[{"name":"Division of Pharmacology and Toxicology ICAR\u2010Indian Veterinary Research Institute Bareilly Uttar Pradesh India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vidya","family":"Singh","sequence":"additional","affiliation":[{"name":"Division of Pathology ICAR\u2010Indian Veterinary Research Institute Bareilly Uttar Pradesh India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Monalisa","family":"Sahoo","sequence":"additional","affiliation":[{"name":"Division of Pathology ICAR\u2010Indian Veterinary Research Institute Bareilly Uttar Pradesh India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dinesh","family":"Kumar","sequence":"additional","affiliation":[{"name":"Division of Pharmacology and Toxicology ICAR\u2010Indian Veterinary Research Institute Bareilly Uttar Pradesh India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,4,4]]},"reference":[{"key":"e_1_2_10_2_1","unstructured":"WHO Cardiovascular Diseases (CVDs). WHO.http:\/\/www.who.int\/mediacentre\/factsheets\/fs317\/en\/\u00a0(accessed: June 11 2021)."},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0178619"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.trsl.2019.03.001"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cellsig.2020.109876"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.4330\/wjc.v10.i9.74"},{"issue":"2","key":"e_1_2_10_7_1","volume":"3","author":"Taso O. V.","year":"2019","journal-title":"Ann. Res. Hosp."},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-020-61238-1"},{"issue":"3","key":"e_1_2_10_9_1","first-page":"4265","volume":"11","author":"Kumar N.","year":"2019","journal-title":"Cureus"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2021.112323"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1080\/10408390903584094"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1177\/15593258211060941"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.27398"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1177\/15593258221114184"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.27398"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.4103\/2221-1691.310201"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.31024\/ajpp.2018.4.1.1"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2019.00793"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1155\/2018\/1960106"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/biof.1514"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2018.10.023"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnins.2017.00657"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1590\/1678-4324-2017160741"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.2174\/1871525718666200206114304"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jafc.7b00967"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/0009-9120(96)00003-6"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(68)90092-4"},{"issue":"3","key":"e_1_2_10_28_1","first-page":"184","volume":"35","author":"Madesh M.","year":"1998","journal-title":"Indian J. Biochem. Biophys."},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0076-6879(84)05016-3"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0076-6879(78)52032-6"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1002\/art.10690"},{"key":"e_1_2_10_32_1","first-page":"258","volume-title":"Manual of Histologic Staining Methods of the Armed Forces Institute of Pathology","author":"Luna L. G.","year":"1968"},{"key":"e_1_2_10_33_1","first-page":"705","volume":"29","author":"Lillie R. D.","year":"1940","journal-title":"Arch. Path."},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1006\/meth.2001.1262"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2017.06.002"},{"issue":"6","key":"e_1_2_10_36_1","first-page":"508","volume":"20","author":"Yuan Y. H.","year":"2018","journal-title":"Chin. J. Contemp. Pediatr."},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.1038\/s12276-018-0121-2"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2019.108618"},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.1080\/01480545.2021.1894707"},{"issue":"2","key":"e_1_2_10_40_1","first-page":"2929","volume":"17","author":"Yang R.","year":"2018","journal-title":"Mol. Med. Rep."},{"issue":"4","key":"e_1_2_10_41_1","first-page":"2028","volume":"24","author":"Dong B.","year":"2020","journal-title":"Eur. Rev. Med. Pharmacol. Sci."},{"key":"e_1_2_10_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fct.2013.04.052"},{"key":"e_1_2_10_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.addr.2019.05.011"},{"key":"e_1_2_10_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phymed.2020.153298"},{"key":"e_1_2_10_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhazmat.2019.121566"},{"issue":"4","key":"e_1_2_10_46_1","first-page":"1635","volume":"8","author":"Sharma M.","year":"2019","journal-title":"J. Pharmacogn. Phytochem."},{"key":"e_1_2_10_47_1","doi-asserted-by":"publisher","DOI":"10.12659\/MSM.918665"},{"key":"e_1_2_10_48_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tifs.2018.07.001"},{"key":"e_1_2_10_49_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms20081820"},{"key":"e_1_2_10_50_1","doi-asserted-by":"publisher","DOI":"10.1161\/HYPERTENSIONAHA.118.12267"},{"key":"e_1_2_10_51_1","doi-asserted-by":"publisher","DOI":"10.7150\/ijbs.28103"},{"key":"e_1_2_10_52_1","doi-asserted-by":"publisher","DOI":"10.1590\/s0102-865020190110000004"},{"key":"e_1_2_10_53_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbi.2016.11.022"},{"key":"e_1_2_10_54_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00059-017-4635-5"},{"key":"e_1_2_10_55_1","author":"Wang Q. W.","year":"2019","journal-title":"J. Evid. Based Complementary Altern. Med."},{"key":"e_1_2_10_56_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2017.00461"},{"key":"e_1_2_10_57_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.HYP.0000208840.30778.00"}],"container-title":["Journal of Biochemical and Molecular Toxicology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jbt.23360","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,13]],"date-time":"2023-09-13T06:57:50Z","timestamp":1694588270000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jbt.23360"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,4,4]]},"references-count":56,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2023,7]]}},"alternative-id":["10.1002\/jbt.23360"],"URL":"https:\/\/doi.org\/10.1002\/jbt.23360","archive":["Portico"],"relation":{},"ISSN":["1095-6670","1099-0461"],"issn-type":[{"value":"1095-6670","type":"print"},{"value":"1099-0461","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,4,4]]},"assertion":[{"value":"2022-05-10","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-03-20","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-04-04","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e23360"}}