{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T15:42:47Z","timestamp":1790005367637,"version":"4.0.1"},"reference-count":31,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2023,6,26]],"date-time":"2023-06-26T00:00:00Z","timestamp":1687737600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Biochem &amp;amp; Molecular Tox"],"published-print":{"date-parts":[[2023,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The study aimed to investigate the hepatoprotective effects of purinergic receptor (P2X7R) antagonism by A438079 in liver damage. An experimental model of inflammation was performed by intraperitoneal (i.p.) lipopolysaccharide (LPS) administration in the rat. The groups were Control, A438079, dimethyl sulfoxide (DMSO), LPS, LPS\u2009+\u2009DMSO, and LPS\u2009+\u2009A438079. Following LPS (8\u2009mg\/kg) injection, A438079 (15\u2009mg\/kg) and DMSO (0.1\u2009mL) were administrated (i.p) in the study groups. The blood and the liver tissues were removed for histological, biochemical, and western blot analyses. In the biochemical analysis, serum aspartate transaminase (AST) and alanine transaminase (ALT) concentrations, the tissue glutathione (GSH) level, and superoxide dismutase (SOD) activity dramatically decreased and malondialdehyde (MDA) level increased in the LPS and LPS\u2009+\u2009DMSO groups compared to the LPS\u2009+\u2009A438079 group. In the histological analysis, severe sinusoidal dilatation, necrotic hepatocytes, and inflammatory cell infiltration were observed in the LPS and LPS\u2009+\u2009DMSO groups while these effects were lessened in the LPS\u2009+\u2009A438079 group. The relative protein expression levels of P2X7R, Nf\u2010kB\u2010p65, IL\u20106, and Caspase\u20103 were significantly higher in the LPS and the LPS\u2009+\u2009DMSO groups than in the LPS\u2009+\u2009A438079 group. On the other hand, these protein expressions were considerably lower in the Control, A438079, and DMSO groups compared to the LPS\u2009+\u2009A438079 group. In addition, Bcl\u20102 protein expression was significantly lower in the LPS and the LPS\u2009+\u2009DMSO groups and higher in the LPS\u2009+\u2009A438079 group compared to the other groups. The protective effect of A438079 against LPS\u2010induced hepatic inflammation may be related to P2X7R antagonism, inflammatory mediators, and apoptotic cell death.<\/jats:p>","DOI":"10.1002\/jbt.23443","type":"journal-article","created":{"date-parts":[[2023,6,27]],"date-time":"2023-06-27T05:11:26Z","timestamp":1687842686000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["Blockade of P2X7 receptor\u2010mediated purinergic signaling with A438079 protects against LPS\u2010induced liver injury in rats"],"prefix":"10.1002","volume":"37","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5766-6116","authenticated-orcid":false,"given":"Adem","family":"Kara","sequence":"first","affiliation":[{"name":"Department of Molecular Biology and Genetics, Faculty of Science Erzurum Technical University Erzurum Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7717-797X","authenticated-orcid":false,"given":"Seckin","family":"Ozkanlar","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Faculty of Veterinary Medicine Atat\u00fcrk University Erzurum Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,6,26]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0278-6915(01)00102-8"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1078-5884(97)80018-4"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.176.7.3877"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/jlb.64.2.265"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V96.3.996"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2013.01.135"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2015.02.011"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.00352.2011"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1093\/toxsci\/kfs283"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1093\/clinchem\/34.3.497"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(68)90092-4"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(79)90738-3"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)52451-6"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.v12.i2.259"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.165.8.4615"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.intimp.2015.04.035"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.toxlet.2020.07.023"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0234038"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1177\/0023677215570087"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.00054.2005"},{"key":"e_1_2_10_22_1","doi-asserted-by":"crossref","unstructured":"S.Ozkanlar N.Ulas O.Kaynar et al. 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