{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T16:17:07Z","timestamp":1791994627134,"version":"4.4.0"},"reference-count":57,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2023,7,2]],"date-time":"2023-07-02T00:00:00Z","timestamp":1688256000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Biochem &amp; Molecular Tox"],"published-print":{"date-parts":[[2023,8]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Saikosaponin\u2010D (SSD), an active ingredient in\n                    <jats:italic>Bupleurum chinense<\/jats:italic>\n                    , exerts anticancer effects in various cancers by inhibiting cancer proliferation and inducing apoptosis. However, whether SSD can induce other forms of cell death is unknown. The current study aims to demonstrate that SSD can induce pyroptosis in non\u2010small\u2010cell lung cancer. In this study, HCC827 and A549 non\u2010small\u2010cell lung cancer cells were treated with different concentrations of SSD for 1.5\u2009h. HE and TUNEL staining were used to verify cell damage caused by SSD. Immunofluorescence and western blotting were performed to verify the effect of SSD on the NF\u2010\u03baB\/NLRP3\/caspase\u20101\/gasdermin D (GSDMD) pathway. Changes in inflammatory factors were detected by ELISAs. Finally, the reactive oxygen species (ROS) scavenger N\u2010acetylcysteine (NAC) was introduced to verify that SSD induces pyroptosis through the ROS\/NF\u2010\u03baB pathway. The results of the HE and TUNEL staining showed that SSD resulted in balloon\u2010like swelling of NSCLC cells accompanied by increased DNA damage. Immunofluorescence and western blot assays confirmed that SSD treatment activated the NLRP3\/caspase\u20101\/GSDMD pathway, stimulated an increase in ROS levels and activated NF\u2010\u03baB in lung cancer cells. The ROS scavenger N\u2010acetylcysteine significantly attenuated SSD\u2010induced NF\u2010\u03baB\/NLRP3\/caspase\u20101\/GSDMD pathway activation and inhibited the release of the inflammatory cytokines IL\u20101\u03b2 and IL\u201018. In conclusion, SSD induced lung cancer cell pyroptosis by inducing ROS accumulation and activating the NF\u2010\u03baB\/NLRP3\/caspase\u20101\/GSDMD pathway. These experiments lay the foundation for the application of SSD in the treatment of non\u2010small\u2010cell lung cancer and regulation of the lung cancer immune microenvironment.\n                  <\/jats:p>","DOI":"10.1002\/jbt.23444","type":"journal-article","created":{"date-parts":[[2023,7,2]],"date-time":"2023-07-02T06:09:07Z","timestamp":1688278147000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":32,"title":["Saikosaponin\u2010D induces the pyroptosis of lung cancer by increasing ROS and activating the NF\u2010\u03baB\/NLRP3\/caspase\u20101\/GSDMD pathway"],"prefix":"10.1002","volume":"37","author":[{"given":"Mengqing","family":"Chen","sequence":"first","affiliation":[{"name":"Department of Respiratory and Critical Care Medicine The Affiliated Hospital of Southwest Medical University  Sichuan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chunyan","family":"Hu","sequence":"additional","affiliation":[{"name":"Department of Respiratory and Critical Care Medicine The Affiliated Hospital of Southwest Medical University  Sichuan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lei","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Respiratory and Critical Care Medicine The First People's Hospital of Neijiang  Sichuan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qingxi","family":"Guo","sequence":"additional","affiliation":[{"name":"Department of Pathology The Affiliated Hospital of Southwest Medical University  Sichuan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuling","family":"Liang","sequence":"additional","affiliation":[{"name":"Department of Respiratory and Critical Care Medicine The Affiliated Hospital of Southwest Medical University  Sichuan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4775-5841","authenticated-orcid":false,"given":"Wenjun","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Respiratory and Critical Care Medicine The Affiliated Hospital of Southwest Medical University  Sichuan China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,7,2]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.3322\/caac.21660"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.3892\/ol.2021.13108"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41392-019-0099-9"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13046-019-1093-3"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1155\/2017\/7597596"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1177\/0300060520937163"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2004.03.008"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.molpharmaceut.8b00595"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.prp.2017.01.022"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2018.09.038"},{"issue":"17","key":"e_1_2_8_12_1","first-page":"2435","volume":"18","author":"Liu R. 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