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The compounds <jats:bold>MAAS\u20105<\/jats:bold> and four modified the supercoiled tertiary structure of pBR322 plasmid DNA. <jats:bold>MAAS\u20105<\/jats:bold> showed the highest cytotoxic activity in HeLa, MCF\u20107, and L929 cells with IC50 values of 16.76\u2009\u00b1\u20093.22, 28.83\u2009\u00b1\u20095.61, and 2.18\u2009\u00b1\u20091.22\u2009\u00b5M, respectively. <jats:bold>MAAS\u20103<\/jats:bold> was found to have almost the lowest cytotoxic activities with the IC50 values of 93.17\u2009\u00b1\u20099.28, 181.07\u2009\u00b1\u200911.54, and 16.86\u2009\u00b1\u20096.42\u2009\u00b5M in HeLa, MCF\u20107, and L929 cells respectively at 24\u2009h. Moreover, the antiepileptic potentials of these compounds were investigated in this study. To this end, the effect of newly synthesized Schiff base derivatives on the enzyme activities of carbonic anhydrase I and II isozymes (human carbonic anhydrase [hCA] I and hCA II) was evaluated spectrophotometrically. The target compounds demonstrated high inhibitory activities compared with standard inhibitors with K<jats:sub>i<\/jats:sub> values in the range of 4.54\u2009\u00b1\u20090.86\u201315.46\u2009\u00b1\u20098.65\u2009nM for hCA I (K<jats:sub>i<\/jats:sub> value for standard inhibitor\u2009=\u2009 12.08\u2009\u00b1\u20092.00\u2009nM), 1.09\u2009\u00b1\u20090.32\u201329.94\u2009\u00b1\u20090.82\u2009nM for hCA II (K<jats:sub>i<\/jats:sub> value for standard inhibitor\u2009=\u200918.22\u2009\u00b1\u20094.90\u2009 nM). Finally, the activities of the compounds were compared with the Gaussian programme in the B3lyp, HF, M062X base sets with 6\u201031++G\u2009(d,p) levels. In addition, the activities of five compounds against various breast cancer proteins and hCA I and II were compared with molecular docking calculations. Also, absorption, distribution, metabolism, excretion, and toxicity analysis was performed to investigate the possibility of using five compounds as drug candidates.<\/jats:p>","DOI":"10.1002\/jbt.23465","type":"journal-article","created":{"date-parts":[[2023,7,18]],"date-time":"2023-07-18T10:55:05Z","timestamp":1689677705000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Piperazin incorporated Schiff Base derivatives: Assessment of in vitro biological activities, metabolic enzyme inhibition properties, and molecular docking calculations"],"prefix":"10.1002","volume":"37","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4789-7180","authenticated-orcid":false,"given":"Arif","family":"Mermer","sequence":"first","affiliation":[{"name":"Experimental Medicine Application &amp; Research Center, Valideba\u011f Research Park University of Health Sciences Istanbul T\u00fcrkiye"},{"name":"Department of Biotechnology 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