{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T09:56:25Z","timestamp":1753869385877,"version":"3.41.2"},"reference-count":47,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2023,7,21]],"date-time":"2023-07-21T00:00:00Z","timestamp":1689897600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100007854","name":"Central Institute of Medicinal and Aromatic Plants","doi-asserted-by":"publisher","award":["DU2\/MLP 11"],"award-info":[{"award-number":["DU2\/MLP 11"]}],"id":[{"id":"10.13039\/501100007854","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Biochem &amp; Molecular Tox"],"published-print":{"date-parts":[[2023,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Flavonoids are among the largest groups of secondary metabolites. Studies suggest\u00a0that dietary intake of flavonoids reduces\u00a0the risk of cancer. 3,5,7\u2010trihydroxyflavone (THF) belongs to the flavone class of flavonoids and potentially inhibits the growth of many cancers; however, it is unexplored in prostate cancer. This study reports the antiproliferative potential of THF in prostate cancer cell line <jats:italic>via<\/jats:italic> reactive oxygen species (ROS)\u2010mediated cascades and examines the tumour reduction potential in swiss albino mice. The potency of THF was evaluated by employing cytotoxicity assays and wound healing assays. Cell cycle, ROS, mitochondrial membrane potential (MMP), and Annexin\u2010V\u2010FITC assay were performed using a flow cytometer. In vivo, anticancer potential was achieved using the mice Ehrlich Ascites Carcinoma (EAC) model. THF inhibits cell growth with IC<jats:sub>50<\/jats:sub> of 64.30\u2009\u00b5M (MTT), 81.22\u2009\u00b5M (NRU) and 25.81\u2009\u00b5M (SRB), substantiated by cell migration assay. Cell\u2010cycle analysis revealed that THF increases the subdiploid population. Furthermore, the Annexin\u2010V\u2010FITC assay evoked a significant induction of late apoptosis at a higher concentration of THF. THF also disrupts MMP, caused by an increased generation of ROS. In the EAC model, THF significantly inhibits tumour growth and increases the percent survival of mice and ROS levels in EAC cells. Hence, it may be concluded that THF might execute its antiproliferative effect via inducing ROS generation and could be a promising lead for preclinical and clinical validations.<\/jats:p>","DOI":"10.1002\/jbt.23474","type":"journal-article","created":{"date-parts":[[2023,7,21]],"date-time":"2023-07-21T11:01:11Z","timestamp":1689937271000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["3,5,7\u2010trihydroxyflavone restricts proliferation of androgen\u2010independent human prostate adenocarcinoma cells by inducing ROS\u2010mediated apoptosis and reduces\u00a0tumour growth"],"prefix":"10.1002","volume":"37","author":[{"given":"Waseem","family":"Raza","sequence":"first","affiliation":[{"name":"Bioprospection and Product Development Division CSIR\u2010Central Institute of Medicinal and Aromatic Plants Lucknow Uttar Pradesh India"},{"name":"Jawaharlal Nehru University  New Delhi India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1014-1486","authenticated-orcid":false,"given":"Abha","family":"Meena","sequence":"additional","affiliation":[{"name":"Bioprospection and Product Development Division CSIR\u2010Central Institute of Medicinal and Aromatic Plants Lucknow Uttar Pradesh India"},{"name":"Academy of Scientific and Innovative Research (AcSIR) Ghaziabad India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6568-8107","authenticated-orcid":false,"given":"Suaib","family":"Luqman","sequence":"additional","affiliation":[{"name":"Bioprospection and Product Development Division CSIR\u2010Central Institute of Medicinal and Aromatic Plants Lucknow Uttar Pradesh India"},{"name":"Academy of Scientific and Innovative Research (AcSIR) Ghaziabad India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2023,7,21]]},"reference":[{"doi-asserted-by":"publisher","key":"e_1_2_11_2_1","DOI":"10.1017\/jns.2016.41"},{"doi-asserted-by":"publisher","key":"e_1_2_11_3_1","DOI":"10.1515\/jbcpp-2019-0191"},{"doi-asserted-by":"publisher","key":"e_1_2_11_4_1","DOI":"10.4103\/0973-7847.79093"},{"doi-asserted-by":"publisher","key":"e_1_2_11_5_1","DOI":"10.1016\/j.urology.2003.10.013"},{"doi-asserted-by":"publisher","key":"e_1_2_11_6_1","DOI":"10.1002\/ijc.33588"},{"doi-asserted-by":"publisher","key":"e_1_2_11_7_1","DOI":"10.3322\/caac.21660"},{"doi-asserted-by":"publisher","key":"e_1_2_11_8_1","DOI":"10.1016\/S1470-2045(01)00486-7"},{"doi-asserted-by":"publisher","key":"e_1_2_11_9_1","DOI":"10.7314\/APJCP.2012.13.2.427"},{"doi-asserted-by":"publisher","key":"e_1_2_11_10_1","DOI":"10.1177\/107327480401100604"},{"doi-asserted-by":"publisher","key":"e_1_2_11_11_1","DOI":"10.1016\/j.jff.2018.04.054"},{"doi-asserted-by":"publisher","key":"e_1_2_11_12_1","DOI":"10.1021\/jf9808224"},{"doi-asserted-by":"publisher","key":"e_1_2_11_13_1","DOI":"10.1159\/000489564"},{"doi-asserted-by":"publisher","key":"e_1_2_11_14_1","DOI":"10.1177\/1535370213497882"},{"doi-asserted-by":"publisher","key":"e_1_2_11_15_1","DOI":"10.1016\/j.tox.2014.09.010"},{"doi-asserted-by":"publisher","key":"e_1_2_11_16_1","DOI":"10.1016\/j.ejphar.2013.08.026"},{"doi-asserted-by":"publisher","key":"e_1_2_11_17_1","DOI":"10.1080\/10286020.2012.682152"},{"doi-asserted-by":"publisher","key":"e_1_2_11_18_1","DOI":"10.1159\/000337041"},{"doi-asserted-by":"publisher","key":"e_1_2_11_19_1","DOI":"10.1007\/s10616-012-9499-1"},{"doi-asserted-by":"publisher","key":"e_1_2_11_20_1","DOI":"10.1080\/14756360601141562"},{"doi-asserted-by":"publisher","key":"e_1_2_11_21_1","DOI":"10.1016\/j.jff.2015.03.051"},{"doi-asserted-by":"publisher","key":"e_1_2_11_22_1","DOI":"10.1016\/j.biopha.2021.111808"},{"doi-asserted-by":"publisher","key":"e_1_2_11_23_1","DOI":"10.1021\/jf010976a"},{"doi-asserted-by":"publisher","key":"e_1_2_11_24_1","DOI":"10.1002\/biof.5520220159"},{"doi-asserted-by":"publisher","key":"e_1_2_11_25_1","DOI":"10.1111\/j.1365-2672.2007.03393.x"},{"doi-asserted-by":"publisher","key":"e_1_2_11_26_1","DOI":"10.1016\/S0378-8741(97)01514-6"},{"doi-asserted-by":"publisher","key":"e_1_2_11_27_1","DOI":"10.1016\/j.ejmech.2014.04.002"},{"doi-asserted-by":"publisher","key":"e_1_2_11_28_1","DOI":"10.3390\/molecules25071579"},{"doi-asserted-by":"publisher","key":"e_1_2_11_29_1","DOI":"10.3892\/or.2017.5767"},{"doi-asserted-by":"publisher","key":"e_1_2_11_30_1","DOI":"10.1016\/j.biopha.2016.10.111"},{"doi-asserted-by":"publisher","key":"e_1_2_11_31_1","DOI":"10.1016\/j.cbi.2017.11.001"},{"doi-asserted-by":"publisher","key":"e_1_2_11_32_1","DOI":"10.1111\/jphp.12753"},{"doi-asserted-by":"publisher","key":"e_1_2_11_33_1","DOI":"10.1038\/nprot.2008.75"},{"doi-asserted-by":"publisher","key":"e_1_2_11_34_1","DOI":"10.1093\/jnci\/82.13.1107"},{"issue":"5","key":"e_1_2_11_35_1","first-page":"85","volume":"73","author":"Wani Z. 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