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Recent findings indicated that ALDH2 also played a critical role\u00a0in detoxification of formaldehyde (FA). Our previous studies showed that FA could enhance macrophagic inflammatory responses through the induction of HIF\u20101\u03b1\u2010dependent glycolysis. In the present study, pro\u2010inflammatory responses and glycolysis promoted by 0.5\u2009mg\/m<jats:sup>3<\/jats:sup> FA were found in mice with Aldh2 gene knockout, which was confirmed in the primary macrophages isolated from Aldh2 gene knockout mice treated with 50\u2009\u03bcM FA. FA at 50 and 100\u2009\u03bcM also induced stronger dose\u2010dependent increases of pro\u2010inflammatory responses and glycolysis in RAW264.7 murine macrophages with knock\u2010down of ALDH2, and the enhanced effects induced by 50\u2009\u03bcM FA was alleviated by inhibition of HIF\u20101\u03b1 in RAW264.7 macrophages with ALDH2 knock\u2010down. Collectively, these results clearly demonstrated that ALDH2 deficiency reinforced pro\u2010inflammatory responses and glycolysis in macrophages potentiated by environmentally relevant concentration of FA, which may increase the susceptibility to inflammation and immunotoxicity induced by environmental FA exposure.<\/jats:p>","DOI":"10.1002\/jbt.23518","type":"journal-article","created":{"date-parts":[[2023,8,28]],"date-time":"2023-08-28T06:44:27Z","timestamp":1693205067000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Aldehyde dehydrogenase 2 deficiency reinforces formaldehyde\u2010potentiated pro\u2010inflammatory responses and glycolysis in macrophages"],"prefix":"10.1002","volume":"38","author":[{"given":"Huijuan","family":"Ma","sequence":"first","affiliation":[{"name":"Shanghai Key Laboratory of New Drug Design, State Key Laboratory of Bioreactor Engineering, School of Pharmacy,\u00a0Shanghai Frontiers 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