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DIZE\u2010treated cells stained with acridine orange\u2010ethidium and JC\u201010 dye revealed cell death and loss of mitochondrial membrane potential (\u03a8m), compared with\u00a0DMSO (vehicle) control, respectively. Cellular immunofluorescence staining of DIZE\u2010treated cells showed upregulation of caspase 3 activities. DIZE\u2010treated cells showed downregulation of mRNA for G1\/S genes CCNA2 and CDC25A, S\u2010phase genes MCM3 and PLK4, and G2\/S phase transition\/mitosis genes Aurka and PLK1. These effects were associated with decreased mRNA expression of Furin, c\u2010Myc, and FOXM1 oncogenes. These results suggested that DIZE may be considered for its effects on other cancer types. To the best of our knowledge, this is the first study to evaluate the effect of DIZE on human cervical cancer cells.<\/jats:p>","DOI":"10.1002\/jbt.23527","type":"journal-article","created":{"date-parts":[[2023,9,8]],"date-time":"2023-09-08T07:17:22Z","timestamp":1694157442000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Diminazene aceturate\u2010induced cytotoxicity is associated with the deregulation of cell cycle signaling and downregulation of oncogenes Furin, c\u2010MYC, and FOXM1 in human cervical carcinoma Hela cells"],"prefix":"10.1002","volume":"38","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2351-3808","authenticated-orcid":false,"given":"Rajendra","family":"Gharbaran","sequence":"first","affiliation":[{"name":"Department of Biological Sciences Bronx Community College\/City University of New York Bronx New York USA"},{"name":"Department of Biological Sciences Lehman College\/City University of New York Bronx New York USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zouberou","family":"Sayibou","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences Bronx Community College\/City University of New York Bronx New York USA"},{"name":"Department of Computer Science Stanford University Stanford California USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Seher","family":"Atamturktur","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences Bronx Community College\/City University of New York Bronx New York USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jeithy Jason","family":"Ofosu\u2010Mensah","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences Bronx Community College\/City University of New York Bronx New York USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John","family":"Soto","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences Lehman College\/City University of New York Bronx New York USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nicholas","family":"Boodhan","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences Lehman College\/City University of New York Bronx New York USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Saaimah","family":"Kolya","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences Lehman College\/City University of New York Bronx New York USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Onyekwere","family":"Onwumere","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences Lehman College\/City University of New York Bronx New York USA"},{"name":"Biology Doctoral Program The Graduate School and University Center, City University of New York New York New York USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lynne","family":"Chang","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences Lehman College\/City University of New York Bronx New York USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Latchman","family":"Somenarain","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences Bronx Community College\/City University of New York Bronx New York USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stephen","family":"Redenti","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences Lehman College\/City University of New York Bronx New York USA"},{"name":"Biology Doctoral Program The Graduate School and University Center, City University of New York New York New York USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2023,9,8]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.3322\/caac.21660"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.103.2675.409"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/179663a0"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00280-009-1120-8"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/205698a0"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199504133321507"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/cam4.3298"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1097\/00129804-200311000-00004"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.4103\/ijri.IJRI_178_19"},{"issue":"1","key":"e_1_2_10_11_1","first-page":"59","volume":"8","author":"Florescu M.","year":"2013","journal-title":"Maedica"},{"issue":"6","key":"e_1_2_10_12_1","first-page":"924","volume":"32","author":"Malyszko J.","year":"2017","journal-title":"Nephrol. 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