{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,8,15]],"date-time":"2025-08-15T02:33:01Z","timestamp":1755225181059,"version":"3.43.0"},"reference-count":61,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2023,9,7]],"date-time":"2023-09-07T00:00:00Z","timestamp":1694044800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Biochem &amp;amp; Molecular Tox"],"published-print":{"date-parts":[[2024,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Interleukin\u20101beta (IL\u20101\u03b2) and interleukin\u201017A (IL\u201017A) have strong pro\u2010inflammatory activities that are involved in inflammatory bowel diseases (IBDs). Mesenchymal stem cell (MSC) therapy is considered a promising treatment for IBD. This study was performed to understand the role of rat <jats:italic>Nlrp3<\/jats:italic> inflammasome, <jats:italic>Hmgb1<\/jats:italic>, and pro\u2010inflammatory cytokines (IL\u20101\u03b2 and IL\u201017a) in the pathogenesis of IBD. Also, to evaluate the role of human umbilical cord blood\u2010MSCs (hUCB\u2010MSCs) in the management of IBD. The rats were in four groups: normal controls, indomethacin\u2010induced IBD group, indomethacin\u2010induced IBD rats that received phosphate\u2010buffered saline (PBS), and the IBD group that received hUCB\u2010MSCs as a treatment. The messenger RNA (mRNA) expression levels of <jats:italic>rat Nlrp3, Hmgb1, IL\u20101\u03b2<\/jats:italic>, and <jats:italic>IL\u201017a<\/jats:italic> were evaluated by quantitative real\u2010time polymerase chain reaction.\u00a0Histopathological examination of the small intestinal tissues of the studied rats was performed. There was a significant upregulation of the <jats:italic>rat Nlrp3<\/jats:italic>, <jats:italic>IL\u20101\u03b2, IL\u201017a<\/jats:italic> mRNA expression (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.001 for the three parameters), and <jats:italic>Hmgb1<\/jats:italic> (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05) in the untreated IBD group compared to the normal control group. In the MSC\u2010treated group, <jats:italic>IL\u20101\u03b2, IL\u201017a<\/jats:italic>, and <jats:italic>rat Nlrp3<\/jats:italic> mRNA expression significantly decreased compared to both the untreated IBD group and PBS group (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05 for all). hUCB\u2010MSCs ameliorated IBD in rats by downregulating the pro\u2010inflammatory cytokines (<jats:italic>IL\u20101\u03b2 and IL\u201017a<\/jats:italic>) and other inflammatory mediators such as <jats:italic>Hmgb1<\/jats:italic> and <jats:italic>rat Nlrp3<\/jats:italic>.<\/jats:p>","DOI":"10.1002\/jbt.23532","type":"journal-article","created":{"date-parts":[[2023,9,7]],"date-time":"2023-09-07T13:08:28Z","timestamp":1694092108000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Therapeutic potentials of mesenchymal stem cells in the treatment of inflammatory bowel disease in rats"],"prefix":"10.1002","volume":"38","author":[{"given":"Shuzan","family":"Ali Mohammed","sequence":"first","affiliation":[{"name":"Department of Medical Biochemistry and Molecular Biology, Faculty of Medicine Benha University Benha Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Azza","family":"Elbaramawy","sequence":"additional","affiliation":[{"name":"Department of Medical Biochemistry and Molecular Biology, Faculty of Medicine Benha University Benha Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Somia","family":"Hassan Abd\u2010Allah","sequence":"additional","affiliation":[{"name":"Department of Medical Biochemistry and Molecular Biology, Faculty of Medicine Zagazig University Zagazig Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Adel","family":"Elkholy","sequence":"additional","affiliation":[{"name":"Department of Medical Biochemistry and Molecular Biology, Faculty of Medicine Benha University Benha Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nashwa","family":"Ibrahim Elsayed","sequence":"additional","affiliation":[{"name":"Department of Medical Biochemistry and Molecular Biology, Faculty of Medicine Benha University Benha Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7407-1608","authenticated-orcid":false,"given":"Samia","family":"Hussein","sequence":"additional","affiliation":[{"name":"Department of Medical Biochemistry and Molecular Biology, Faculty of Medicine Zagazig University Zagazig Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2023,9,7]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2017.00942"},{"key":"e_1_2_10_3_1","first-page":"39","volume":"15","author":"Zherebiatiev A.","year":"2016","journal-title":"Iran. 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