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The current study was to explore the potential relationship between the miR\u20103845\/TRIM35\/PKM2 and abnormal polarization of mononuclear macrophages. Using bioinformatics to analyze the gene expression of mononuclear macrophages. The polarization of macrophages was analyzed using flow cytometry, and the expression changes of TRIM35\/PKM2 were analyzed using Western blot, luciferase activity assay, and co\u2010immunoprecipitation. Database analysis showed that T1DM patients showed an abnormal increase of miR\u20103945 in CD14+ monocyte macrophages. miR\u20103945 targets TRIM35 to release PKM2 to cytometry, and PKM2 causes M1\u2010like polarization of mononuclear macrophages. Database analysis showed that miR\u20103945 was abnormally upregulated in CD14+ monocytes in T1DM patients. miR\u20103945 upregulates the expression of PKM2 in the cytoplasm by targeting TRIM35, which leads to M1 polarization of macrophages.<\/jats:p>","DOI":"10.1002\/jbt.23539","type":"journal-article","created":{"date-parts":[[2023,9,13]],"date-time":"2023-09-13T05:42:45Z","timestamp":1694583765000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["The upregulation of miR\u20103945 in CD14+ monocytes is the risk factor for cardiovascular disease"],"prefix":"10.1002","volume":"38","author":[{"given":"Wei","family":"Xu","sequence":"first","affiliation":[{"name":"Department of Cardiology Huadu District People's Hospital of Guangzhou Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tingting","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine The First Dongguan Affiliated Hospital, Guangdong Medical University Dongguan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1309-198X","authenticated-orcid":false,"given":"Xin","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of ECG Huadu District People's Hospital of Guangzhou Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,9,13]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrendo.2017.116"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tcm.2015.05.005"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.118.036068"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2020.117559"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1161\/ATVBAHA.120.314150"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms19103242"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biocel.2017.01.009"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.2337\/db17-1434"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12967-019-1977-1"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.7150\/ijbs.15659"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.2337\/diabetes.54.9.2779"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1042\/CS20160998"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.118.035525"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.2174\/1573399812666151016101622"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1042\/BST20160325"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tibs.2017.01.002"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2007-04-085746"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.15252\/embr.201643300"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13578-019-0317-8"}],"container-title":["Journal of Biochemical and Molecular Toxicology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jbt.23539","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,8,13]],"date-time":"2025-08-13T07:48:46Z","timestamp":1755071326000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jbt.23539"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,9,13]]},"references-count":19,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2024,1]]}},"alternative-id":["10.1002\/jbt.23539"],"URL":"https:\/\/doi.org\/10.1002\/jbt.23539","archive":["Portico"],"relation":{},"ISSN":["1095-6670","1099-0461"],"issn-type":[{"value":"1095-6670","type":"print"},{"value":"1099-0461","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,9,13]]},"assertion":[{"value":"2023-01-01","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-09-01","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-09-13","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e23539"}}